The reproductive toxicity of CdSe/ZnS quantum dots on the in vivo ovarian function and in vitro fertilization
Despite the usefulness of quantum dots (QDs) in biomedicine and optoelectronics, their toxicity risks remain a major obstacle for clinical usages. Hence, we studied the reproductive toxicity of CdSe/ZnS QDs on two aspects, (i) in vivo ovarian functions and (ii) in vitro fertilization process. The bo...
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sg-ntu-dr.10356-876272020-03-07T13:57:31Z The reproductive toxicity of CdSe/ZnS quantum dots on the in vivo ovarian function and in vitro fertilization Xu, Gaixia Lin, Guimiao Lin, Suxia Wu, Na Deng, Yueyue Feng, Gang Chen, Qiang Qu, Junle Chen, Danni Chen, Siping Niu, Hanben Mei, Shujiang Yong, Ken-Tye Wang, Xiaomei School of Electrical and Electronic Engineering DRNTU::Engineering::Electrical and electronic engineering Quantum Dots Fertilization in Vitro Despite the usefulness of quantum dots (QDs) in biomedicine and optoelectronics, their toxicity risks remain a major obstacle for clinical usages. Hence, we studied the reproductive toxicity of CdSe/ZnS QDs on two aspects, (i) in vivo ovarian functions and (ii) in vitro fertilization process. The body weight, estrous cycles, biodistribution of QDs, and oocyte maturation are evaluated on female mice treated with QDs. The mRNA level of the follicle-stimulating hormone receptor (FSHr) and luteinizing hormone receptor (LHr) in ovaries are assayed. Then, the matured cumulus-oocyte-complexes are harvested to co-culture with in vitro capacitated sperms, and the in vitro fertilization is performed. The result revealed that QDs are found in the ovaries, but no changes are detected on the behavior and estrous cycle on the female mice. The mRNA downregulations of FSHr and LHr are observed and the number of matured oocytes has shown a significant decrease when the QDs dosage was above 1.0 pmol/day. Additionally, we found the presence of QDs has reduced the in vitro fertilization success rate. This study highly suggests that the exposure of CdSe/ZnS QDs to female mice can cause adverse effects to the ovary functions and such QDs may have limited applications in clinical usage. Published version 2018-11-30T08:10:13Z 2019-12-06T16:45:58Z 2018-11-30T08:10:13Z 2019-12-06T16:45:58Z 2016 Journal Article Xu, G., Lin, G., Lin, S., Wu, N., Deng, Y., Feng, G., . . . Wang, X. (2016). The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization. Scientific Reports, 6, 37677-. doi:10.1038/srep37677 https://hdl.handle.net/10356/87627 http://hdl.handle.net/10220/46759 10.1038/srep37677 en Scientific Reports © 2016 The Authors (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 11 p. application/pdf |
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DRNTU::Engineering::Electrical and electronic engineering Quantum Dots Fertilization in Vitro Xu, Gaixia Lin, Guimiao Lin, Suxia Wu, Na Deng, Yueyue Feng, Gang Chen, Qiang Qu, Junle Chen, Danni Chen, Siping Niu, Hanben Mei, Shujiang Yong, Ken-Tye Wang, Xiaomei The reproductive toxicity of CdSe/ZnS quantum dots on the in vivo ovarian function and in vitro fertilization |
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Despite the usefulness of quantum dots (QDs) in biomedicine and optoelectronics, their toxicity risks remain a major obstacle for clinical usages. Hence, we studied the reproductive toxicity of CdSe/ZnS QDs on two aspects, (i) in vivo ovarian functions and (ii) in vitro fertilization process. The body weight, estrous cycles, biodistribution of QDs, and oocyte maturation are evaluated on female mice treated with QDs. The mRNA level of the follicle-stimulating hormone receptor (FSHr) and luteinizing hormone receptor (LHr) in ovaries are assayed. Then, the matured cumulus-oocyte-complexes are harvested to co-culture with in vitro capacitated sperms, and the in vitro fertilization is performed. The result revealed that QDs are found in the ovaries, but no changes are detected on the behavior and estrous cycle on the female mice. The mRNA downregulations of FSHr and LHr are observed and the number of matured oocytes has shown a significant decrease when the QDs dosage was above 1.0 pmol/day. Additionally, we found the presence of QDs has reduced the in vitro fertilization success rate. This study highly suggests that the exposure of CdSe/ZnS QDs to female mice can cause adverse effects to the ovary functions and such QDs may have limited applications in clinical usage. |
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School of Electrical and Electronic Engineering |
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School of Electrical and Electronic Engineering Xu, Gaixia Lin, Guimiao Lin, Suxia Wu, Na Deng, Yueyue Feng, Gang Chen, Qiang Qu, Junle Chen, Danni Chen, Siping Niu, Hanben Mei, Shujiang Yong, Ken-Tye Wang, Xiaomei |
format |
Article |
author |
Xu, Gaixia Lin, Guimiao Lin, Suxia Wu, Na Deng, Yueyue Feng, Gang Chen, Qiang Qu, Junle Chen, Danni Chen, Siping Niu, Hanben Mei, Shujiang Yong, Ken-Tye Wang, Xiaomei |
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Xu, Gaixia |
title |
The reproductive toxicity of CdSe/ZnS quantum dots on the in vivo ovarian function and in vitro fertilization |
title_short |
The reproductive toxicity of CdSe/ZnS quantum dots on the in vivo ovarian function and in vitro fertilization |
title_full |
The reproductive toxicity of CdSe/ZnS quantum dots on the in vivo ovarian function and in vitro fertilization |
title_fullStr |
The reproductive toxicity of CdSe/ZnS quantum dots on the in vivo ovarian function and in vitro fertilization |
title_full_unstemmed |
The reproductive toxicity of CdSe/ZnS quantum dots on the in vivo ovarian function and in vitro fertilization |
title_sort |
reproductive toxicity of cdse/zns quantum dots on the in vivo ovarian function and in vitro fertilization |
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2018 |
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https://hdl.handle.net/10356/87627 http://hdl.handle.net/10220/46759 |
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1681035417357385728 |