Non-linear optical microscopy and histological analysis of collagen, elastin and lysyl oxidase expression in breast capsular contracture

Background: Capsular contracture is one of the most common complications in surgical interventions for aesthetic breast augmentation or post-mastectomy breast reconstruction involving the use of silicone prostheses. Although the precise cause of capsular contracture is yet unknown, the leading hypot...

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Main Authors: Poh, Patrina S. P., Schmauss, Verena, McGovern, Jacqui A., Schmauss, Daniel, Chhaya, Mohit P., Foehr, Peter, Seeger, Markus, Ntziachristos, Vasilis, Hutmacher, Dietmar W., van Griensven, Martijn, Schantz, Jan-Thorsten, Balmayor, Elizabeth R.
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2018
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Online Access:https://hdl.handle.net/10356/87762
http://hdl.handle.net/10220/45522
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-877622023-12-29T06:47:18Z Non-linear optical microscopy and histological analysis of collagen, elastin and lysyl oxidase expression in breast capsular contracture Poh, Patrina S. P. Schmauss, Verena McGovern, Jacqui A. Schmauss, Daniel Chhaya, Mohit P. Foehr, Peter Seeger, Markus Ntziachristos, Vasilis Hutmacher, Dietmar W. van Griensven, Martijn Schantz, Jan-Thorsten Balmayor, Elizabeth R. School of Chemical and Biomedical Engineering Silicone Breast Implant Collagen Background: Capsular contracture is one of the most common complications in surgical interventions for aesthetic breast augmentation or post-mastectomy breast reconstruction involving the use of silicone prostheses. Although the precise cause of capsular contracture is yet unknown, the leading hypothesis is that it is caused by long-term unresolved foreign body reaction towards the silicone breast implant. To authors’ best knowledge, this is the first study that elucidates the presence of lysyl oxidase (LOX)—an enzyme that is involved in collagen and elastin crosslinking within fibrous capsules harvested from patients with severe capsular contracture. It was hypothesized that over-expression of LOX plays a role in the irreversible crosslinking of collagen and elastin which, in turn, stabilizes the fibrous proteins and contributes to the progression of capsular contracture. Methods: Eight fibrous capsules were collected from patients undergoing capsulectomy procedure, biomechanical testing was performed for compressive Young’s moduli and evaluated for Type I and II collagen, elastin and LOX by means of non-linear optical microscopy and immunohistology techniques. Results: Observations revealed the heterogeneity of tissue structure within and among the collected fibrous capsules. Regardless of the tissue structure, it has been shown that LOX expression was intensified at the implant-to-tissue interface. Conclusion: Our results indicate the involvement of LOX in the initiation of fibrous capsule formation which ultimately contributes towards the progression of capsular contracture. Published version 2018-08-07T06:54:39Z 2019-12-06T16:48:55Z 2018-08-07T06:54:39Z 2019-12-06T16:48:55Z 2018 Journal Article Poh, P. S. P., Schmauss, V., McGovern, J. A., Schmauss, D., Chhaya, M. P., Foehr, P., et al. (2018). Non-linear optical microscopy and histological analysis of collagen, elastin and lysyl oxidase expression in breast capsular contracture. European Journal of Medical Research, 23(1), 30-. 0949-2321 https://hdl.handle.net/10356/87762 http://hdl.handle.net/10220/45522 10.1186/s40001-018-0322-0 en European Journal of Medical Research © 2018 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. 11 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Silicone Breast Implant
Collagen
spellingShingle Silicone Breast Implant
Collagen
Poh, Patrina S. P.
Schmauss, Verena
McGovern, Jacqui A.
Schmauss, Daniel
Chhaya, Mohit P.
Foehr, Peter
Seeger, Markus
Ntziachristos, Vasilis
Hutmacher, Dietmar W.
van Griensven, Martijn
Schantz, Jan-Thorsten
Balmayor, Elizabeth R.
Non-linear optical microscopy and histological analysis of collagen, elastin and lysyl oxidase expression in breast capsular contracture
description Background: Capsular contracture is one of the most common complications in surgical interventions for aesthetic breast augmentation or post-mastectomy breast reconstruction involving the use of silicone prostheses. Although the precise cause of capsular contracture is yet unknown, the leading hypothesis is that it is caused by long-term unresolved foreign body reaction towards the silicone breast implant. To authors’ best knowledge, this is the first study that elucidates the presence of lysyl oxidase (LOX)—an enzyme that is involved in collagen and elastin crosslinking within fibrous capsules harvested from patients with severe capsular contracture. It was hypothesized that over-expression of LOX plays a role in the irreversible crosslinking of collagen and elastin which, in turn, stabilizes the fibrous proteins and contributes to the progression of capsular contracture. Methods: Eight fibrous capsules were collected from patients undergoing capsulectomy procedure, biomechanical testing was performed for compressive Young’s moduli and evaluated for Type I and II collagen, elastin and LOX by means of non-linear optical microscopy and immunohistology techniques. Results: Observations revealed the heterogeneity of tissue structure within and among the collected fibrous capsules. Regardless of the tissue structure, it has been shown that LOX expression was intensified at the implant-to-tissue interface. Conclusion: Our results indicate the involvement of LOX in the initiation of fibrous capsule formation which ultimately contributes towards the progression of capsular contracture.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Poh, Patrina S. P.
Schmauss, Verena
McGovern, Jacqui A.
Schmauss, Daniel
Chhaya, Mohit P.
Foehr, Peter
Seeger, Markus
Ntziachristos, Vasilis
Hutmacher, Dietmar W.
van Griensven, Martijn
Schantz, Jan-Thorsten
Balmayor, Elizabeth R.
format Article
author Poh, Patrina S. P.
Schmauss, Verena
McGovern, Jacqui A.
Schmauss, Daniel
Chhaya, Mohit P.
Foehr, Peter
Seeger, Markus
Ntziachristos, Vasilis
Hutmacher, Dietmar W.
van Griensven, Martijn
Schantz, Jan-Thorsten
Balmayor, Elizabeth R.
author_sort Poh, Patrina S. P.
title Non-linear optical microscopy and histological analysis of collagen, elastin and lysyl oxidase expression in breast capsular contracture
title_short Non-linear optical microscopy and histological analysis of collagen, elastin and lysyl oxidase expression in breast capsular contracture
title_full Non-linear optical microscopy and histological analysis of collagen, elastin and lysyl oxidase expression in breast capsular contracture
title_fullStr Non-linear optical microscopy and histological analysis of collagen, elastin and lysyl oxidase expression in breast capsular contracture
title_full_unstemmed Non-linear optical microscopy and histological analysis of collagen, elastin and lysyl oxidase expression in breast capsular contracture
title_sort non-linear optical microscopy and histological analysis of collagen, elastin and lysyl oxidase expression in breast capsular contracture
publishDate 2018
url https://hdl.handle.net/10356/87762
http://hdl.handle.net/10220/45522
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