Quantitative optical coherence microscopy for the in situ investigation of the biofilm

Quantitative optical coherence microscopy for the in situ investigation of the biofilm

This paper explores the potential of optical coherence microscopy (OCM) for the in situ monitoring of biofilm growth. The quantitative imaging of the early developmental biology of a representative biofilm, Klebsiella pneumonia (KP-1), was performed using a swept source-based Fourier domain OCM syst...

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Bibliographic Details
Main Authors: Meleppat, Ratheesh Kumar, Shearwood, Christopher, Keey, Seah Leong, Matham, Murukeshan Vadakke
Other Authors: School of Mechanical and Aerospace Engineering
Format: Article
Language:English
Published: 2018
Subjects:
Biofilms
Optical Coherence Tomography
DRNTU::Engineering::Mechanical engineering
Online Access:https://hdl.handle.net/10356/87912
http://hdl.handle.net/10220/46879
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Institution: Nanyang Technological University
Language: English
Description
Summary:This paper explores the potential of optical coherence microscopy (OCM) for the in situ monitoring of biofilm growth. The quantitative imaging of the early developmental biology of a representative biofilm, Klebsiella pneumonia (KP-1), was performed using a swept source-based Fourier domain OCM system. The growth dynamics of the KP-1 biofilms and their transient response under perturbation was investigated using the enface visualization of microcolonies and their spatial localization. Furthermore, the optical density (OD) and planar density of the biofilms are calculated using an OCM technique and compared with OD and colony forming units measured using standard procedures via the sampling of the flow-cell effluent.

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