Progress in exosome isolation techniques

Exosomes are one type of membrane vesicles secreted into extracellular space by most types of cells. In addition to performing many biological functions particularly in cell-cell communication, cumulative evidence has suggested that several biological entities in exosomes like proteins and microRNAs...

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Main Authors: Li, Pin, Kaslan, Melisa, Lee, Sze Han, Yao, Justin, Gao, Zhiqiang
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2018
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Online Access:https://hdl.handle.net/10356/87965
http://hdl.handle.net/10220/45573
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-879652020-11-01T05:20:41Z Progress in exosome isolation techniques Li, Pin Kaslan, Melisa Lee, Sze Han Yao, Justin Gao, Zhiqiang Lee Kong Chian School of Medicine (LKCMedicine) Isolation Exosome Exosomes are one type of membrane vesicles secreted into extracellular space by most types of cells. In addition to performing many biological functions particularly in cell-cell communication, cumulative evidence has suggested that several biological entities in exosomes like proteins and microRNAs are closely associated with the pathogenesis of most human malignancies and they may serve as invaluable biomarkers for disease diagnosis, prognosis, and therapy. This provides a commanding impetus and growing demands for simple, efficient, and affordable techniques to isolate exosomes. Capitalizing on the physicochemical and biochemical properties of exosomes, a number of techniques have been developed for the isolation of exosomes. This article summarizes the advances in exosome isolation techniques with an emphasis on their isolation mechanism, performance, challenges, and prospects. We hope that this article will provide an overview of exosome isolation techniques, opening up new perspectives towards the development more innovative strategies and devices for more time saving, cost effective, and efficient isolations of exosomes from a wide range of biological matrices. MOE (Min. of Education, S’pore) Published version 2018-08-16T06:23:29Z 2019-12-06T16:53:06Z 2018-08-16T06:23:29Z 2019-12-06T16:53:06Z 2017 Journal Article Li, P., Kaslan, M., Lee, S. H., Yao, J., & Gao, Z. (2017). Progress in exosome isolation techniques. Theranostics, 7(3), 789-804. https://hdl.handle.net/10356/87965 http://hdl.handle.net/10220/45573 10.7150/thno.18133 en Theranostics © Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. 16 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Isolation
Exosome
spellingShingle Isolation
Exosome
Li, Pin
Kaslan, Melisa
Lee, Sze Han
Yao, Justin
Gao, Zhiqiang
Progress in exosome isolation techniques
description Exosomes are one type of membrane vesicles secreted into extracellular space by most types of cells. In addition to performing many biological functions particularly in cell-cell communication, cumulative evidence has suggested that several biological entities in exosomes like proteins and microRNAs are closely associated with the pathogenesis of most human malignancies and they may serve as invaluable biomarkers for disease diagnosis, prognosis, and therapy. This provides a commanding impetus and growing demands for simple, efficient, and affordable techniques to isolate exosomes. Capitalizing on the physicochemical and biochemical properties of exosomes, a number of techniques have been developed for the isolation of exosomes. This article summarizes the advances in exosome isolation techniques with an emphasis on their isolation mechanism, performance, challenges, and prospects. We hope that this article will provide an overview of exosome isolation techniques, opening up new perspectives towards the development more innovative strategies and devices for more time saving, cost effective, and efficient isolations of exosomes from a wide range of biological matrices.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Li, Pin
Kaslan, Melisa
Lee, Sze Han
Yao, Justin
Gao, Zhiqiang
format Article
author Li, Pin
Kaslan, Melisa
Lee, Sze Han
Yao, Justin
Gao, Zhiqiang
author_sort Li, Pin
title Progress in exosome isolation techniques
title_short Progress in exosome isolation techniques
title_full Progress in exosome isolation techniques
title_fullStr Progress in exosome isolation techniques
title_full_unstemmed Progress in exosome isolation techniques
title_sort progress in exosome isolation techniques
publishDate 2018
url https://hdl.handle.net/10356/87965
http://hdl.handle.net/10220/45573
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