A yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein–protein interactions identifies a novel putative Mdm2-binding site in p53

• Main Authors: Wong, Jin Huei, Alfatah, Mohammad, Sin, Mei Fang, Sim, Hong May, Verma, Chandra Shekhar, Lane, David P., Arumugam, Prakash
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2018
• Subjects: Yeast Two-hybrid Assay; Protein–protein Interaction Inhibitors
• Online Access: https://hdl.handle.net/10356/88055; http://hdl.handle.net/10220/44532
• Institution: Nanyang Technological University

Background: Protein–protein interactions (PPIs) are fundamental to the growth and survival of cells and serve as excellent targets to develop inhibitors of biological processes such as host-pathogen interactions and cancer cell proliferation. However, isolation of PPI inhibitors is extremely challen...