Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis
Development of molecular probes for the detection of reactive oxygen and nitrogen species (RONS) is important for the pathology and diagnosis of diseases. Although an abnormally high RONS level has been identified in keloids – a benign dermal tumour developed after lesion, the ability of employing R...
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sg-ntu-dr.10356-883832023-12-29T06:47:14Z Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis Cheng, Penghui Zhang, Jianjian Huang, Jiaguo Miao, Qingqing Xu, Chenjie Pu, Kanyi School of Chemical and Biomedical Engineering Cell Culture Diagnosis DRNTU::Engineering::Chemical engineering Development of molecular probes for the detection of reactive oxygen and nitrogen species (RONS) is important for the pathology and diagnosis of diseases. Although an abnormally high RONS level has been identified in keloids – a benign dermal tumour developed after lesion, the ability of employing RONS probes for keloid detection has not yet been exploited. Herein, we report two near-infrared (NIR) fluorescent probes (CyTF and CyBA) that can specifically distinguish keloid fibroblasts from normal dermal fibroblasts. Both CyTF and CyBA show a 15-fold NIR fluorescence enhancement at 717 nm upon reaction with RONS. However, because CyTF has higher specificity towards ONOO− than CyBA, CyTF can detect stimulated fibroblasts in a more sensitive way, showing 3.76 and 2.26-fold fluorescence increments in TGF-β1 stimulated dermal fibroblasts and keloid fibroblasts, respectively. Furthermore, CyTF permits specific detection of implanted keloid fibroblasts in a xenograft live mouse model. Our work thus developed a new optical imaging approach that has the potential for early diagnosis and drug screening of keloids. ASTAR (Agency for Sci., Tech. and Research, S’pore) MOE (Min. of Education, S’pore) Published version 2018-08-29T05:10:36Z 2019-12-06T17:02:03Z 2018-08-29T05:10:36Z 2019-12-06T17:02:03Z 2018 Journal Article Cheng, P., Zhang, J., Huang, J., Miao, Q., Xu, C., & Pu, K. (2018). Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis. Chemical Science, 9(30), 6340-6347. doi:10.1039/c8sc01865k 2041-6520 https://hdl.handle.net/10356/88383 http://hdl.handle.net/10220/45719 10.1039/C8SC01865K en Chemical Science © 2018 The Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. 8 p. application/pdf |
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Cell Culture Diagnosis DRNTU::Engineering::Chemical engineering Cheng, Penghui Zhang, Jianjian Huang, Jiaguo Miao, Qingqing Xu, Chenjie Pu, Kanyi Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis |
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Development of molecular probes for the detection of reactive oxygen and nitrogen species (RONS) is important for the pathology and diagnosis of diseases. Although an abnormally high RONS level has been identified in keloids – a benign dermal tumour developed after lesion, the ability of employing RONS probes for keloid detection has not yet been exploited. Herein, we report two near-infrared (NIR) fluorescent probes (CyTF and CyBA) that can specifically distinguish keloid fibroblasts from normal dermal fibroblasts. Both CyTF and CyBA show a 15-fold NIR fluorescence enhancement at 717 nm upon reaction with RONS. However, because CyTF has higher specificity towards ONOO− than CyBA, CyTF can detect stimulated fibroblasts in a more sensitive way, showing 3.76 and 2.26-fold fluorescence increments in TGF-β1 stimulated dermal fibroblasts and keloid fibroblasts, respectively. Furthermore, CyTF permits specific detection of implanted keloid fibroblasts in a xenograft live mouse model. Our work thus developed a new optical imaging approach that has the potential for early diagnosis and drug screening of keloids. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Cheng, Penghui Zhang, Jianjian Huang, Jiaguo Miao, Qingqing Xu, Chenjie Pu, Kanyi |
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Article |
author |
Cheng, Penghui Zhang, Jianjian Huang, Jiaguo Miao, Qingqing Xu, Chenjie Pu, Kanyi |
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Cheng, Penghui |
title |
Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis |
title_short |
Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis |
title_full |
Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis |
title_fullStr |
Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis |
title_full_unstemmed |
Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis |
title_sort |
near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis |
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2018 |
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https://hdl.handle.net/10356/88383 http://hdl.handle.net/10220/45719 |
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1787136518708527104 |