Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues
Three-dimensional, protein-based hydrogel scaffolds that successfully mimic in vivo extracellular matrix microenvironments are desirable for tissue engineering and regenerative medicine applications, and can provide highly capable in vitro tissue analogues. However, the fabrication of protein-based...
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sg-ntu-dr.10356-885422023-07-14T15:52:14Z Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues Lee, Bae Hoon Shirahama, Hitomi Kim, Myung Hee Lee, Jae Ho Cho, Nam-Joon Tan, Lay Poh School of Chemical and Biomedical Engineering School of Materials Science & Engineering Colloidal Templating Tissue Analogues DRNTU::Engineering::Materials Three-dimensional, protein-based hydrogel scaffolds that successfully mimic in vivo extracellular matrix microenvironments are desirable for tissue engineering and regenerative medicine applications, and can provide highly capable in vitro tissue analogues. However, the fabrication of protein-based scaffolds with uniform porosity, thin walls and durable mechanical properties remains a challenging prospect that might be overcome by integrating advances in microfabrication and protein functionalization. Towards this goal, herein, we report the successful fabrication of a highly ordered, gelatin-based inverted colloidal crystal (ICC) hydrogel platform that is robust and supports high levels of cell function. In particular, the utilization of colloidal templating microfabrication strategies together with highly substituted, photocrosslinkable gelatin methacryloyl (GelMA) allowed us to fabricate protein-based three-dimensional scaffolds with uniform pore interconnectivity, structural stability and tailorable degradation properties. The resulting GelMA ICC scaffolds provided cell attachment sites and promoted intercellular interaction of hepatocytes, which resulted in improved cell function compared to a flat 2D system. The results demonstrate the potential of GelMA ICC scaffolds to become an effective tissue engineering platform for drug screening and regenerative medicine. NRF (Natl Research Foundation, S’pore) Published version 2018-09-05T02:56:40Z 2019-12-06T17:05:39Z 2018-09-05T02:56:40Z 2019-12-06T17:05:39Z 2017 Journal Article Lee, B. H., Shirahama, H., Kim, M. H., Lee, J. H., Cho, N.-J., & Tan, L. P. (2017). Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues. NPG Asia Materials, 9(7), e412-. doi:10.1038/am.2017.126 1884-4049 https://hdl.handle.net/10356/88542 http://hdl.handle.net/10220/45821 10.1038/am.2017.126 en NPG Asia Materials © 2017 The Author(s) (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 11 p. application/pdf |
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Colloidal Templating Tissue Analogues DRNTU::Engineering::Materials Lee, Bae Hoon Shirahama, Hitomi Kim, Myung Hee Lee, Jae Ho Cho, Nam-Joon Tan, Lay Poh Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues |
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Three-dimensional, protein-based hydrogel scaffolds that successfully mimic in vivo extracellular matrix microenvironments are desirable for tissue engineering and regenerative medicine applications, and can provide highly capable in vitro tissue analogues. However, the fabrication of protein-based scaffolds with uniform porosity, thin walls and durable mechanical properties remains a challenging prospect that might be overcome by integrating advances in microfabrication and protein functionalization. Towards this goal, herein, we report the successful fabrication of a highly ordered, gelatin-based inverted colloidal crystal (ICC) hydrogel platform that is robust and supports high levels of cell function. In particular, the utilization of colloidal templating microfabrication strategies together with highly substituted, photocrosslinkable gelatin methacryloyl (GelMA) allowed us to fabricate protein-based three-dimensional scaffolds with uniform pore interconnectivity, structural stability and tailorable degradation properties. The resulting GelMA ICC scaffolds provided cell attachment sites and promoted intercellular interaction of hepatocytes, which resulted in improved cell function compared to a flat 2D system. The results demonstrate the potential of GelMA ICC scaffolds to become an effective tissue engineering platform for drug screening and regenerative medicine. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Lee, Bae Hoon Shirahama, Hitomi Kim, Myung Hee Lee, Jae Ho Cho, Nam-Joon Tan, Lay Poh |
format |
Article |
author |
Lee, Bae Hoon Shirahama, Hitomi Kim, Myung Hee Lee, Jae Ho Cho, Nam-Joon Tan, Lay Poh |
author_sort |
Lee, Bae Hoon |
title |
Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues |
title_short |
Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues |
title_full |
Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues |
title_fullStr |
Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues |
title_full_unstemmed |
Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues |
title_sort |
colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues |
publishDate |
2018 |
url |
https://hdl.handle.net/10356/88542 http://hdl.handle.net/10220/45821 |
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1772826511359868928 |