Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues

Three-dimensional, protein-based hydrogel scaffolds that successfully mimic in vivo extracellular matrix microenvironments are desirable for tissue engineering and regenerative medicine applications, and can provide highly capable in vitro tissue analogues. However, the fabrication of protein-based...

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Main Authors: Lee, Bae Hoon, Shirahama, Hitomi, Kim, Myung Hee, Lee, Jae Ho, Cho, Nam-Joon, Tan, Lay Poh
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2018
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Online Access:https://hdl.handle.net/10356/88542
http://hdl.handle.net/10220/45821
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-885422023-07-14T15:52:14Z Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues Lee, Bae Hoon Shirahama, Hitomi Kim, Myung Hee Lee, Jae Ho Cho, Nam-Joon Tan, Lay Poh School of Chemical and Biomedical Engineering School of Materials Science & Engineering Colloidal Templating Tissue Analogues DRNTU::Engineering::Materials Three-dimensional, protein-based hydrogel scaffolds that successfully mimic in vivo extracellular matrix microenvironments are desirable for tissue engineering and regenerative medicine applications, and can provide highly capable in vitro tissue analogues. However, the fabrication of protein-based scaffolds with uniform porosity, thin walls and durable mechanical properties remains a challenging prospect that might be overcome by integrating advances in microfabrication and protein functionalization. Towards this goal, herein, we report the successful fabrication of a highly ordered, gelatin-based inverted colloidal crystal (ICC) hydrogel platform that is robust and supports high levels of cell function. In particular, the utilization of colloidal templating microfabrication strategies together with highly substituted, photocrosslinkable gelatin methacryloyl (GelMA) allowed us to fabricate protein-based three-dimensional scaffolds with uniform pore interconnectivity, structural stability and tailorable degradation properties. The resulting GelMA ICC scaffolds provided cell attachment sites and promoted intercellular interaction of hepatocytes, which resulted in improved cell function compared to a flat 2D system. The results demonstrate the potential of GelMA ICC scaffolds to become an effective tissue engineering platform for drug screening and regenerative medicine. NRF (Natl Research Foundation, S’pore) Published version 2018-09-05T02:56:40Z 2019-12-06T17:05:39Z 2018-09-05T02:56:40Z 2019-12-06T17:05:39Z 2017 Journal Article Lee, B. H., Shirahama, H., Kim, M. H., Lee, J. H., Cho, N.-J., & Tan, L. P. (2017). Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues. NPG Asia Materials, 9(7), e412-. doi:10.1038/am.2017.126 1884-4049 https://hdl.handle.net/10356/88542 http://hdl.handle.net/10220/45821 10.1038/am.2017.126 en NPG Asia Materials © 2017 The Author(s) (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 11 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Colloidal Templating
Tissue Analogues
DRNTU::Engineering::Materials
spellingShingle Colloidal Templating
Tissue Analogues
DRNTU::Engineering::Materials
Lee, Bae Hoon
Shirahama, Hitomi
Kim, Myung Hee
Lee, Jae Ho
Cho, Nam-Joon
Tan, Lay Poh
Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues
description Three-dimensional, protein-based hydrogel scaffolds that successfully mimic in vivo extracellular matrix microenvironments are desirable for tissue engineering and regenerative medicine applications, and can provide highly capable in vitro tissue analogues. However, the fabrication of protein-based scaffolds with uniform porosity, thin walls and durable mechanical properties remains a challenging prospect that might be overcome by integrating advances in microfabrication and protein functionalization. Towards this goal, herein, we report the successful fabrication of a highly ordered, gelatin-based inverted colloidal crystal (ICC) hydrogel platform that is robust and supports high levels of cell function. In particular, the utilization of colloidal templating microfabrication strategies together with highly substituted, photocrosslinkable gelatin methacryloyl (GelMA) allowed us to fabricate protein-based three-dimensional scaffolds with uniform pore interconnectivity, structural stability and tailorable degradation properties. The resulting GelMA ICC scaffolds provided cell attachment sites and promoted intercellular interaction of hepatocytes, which resulted in improved cell function compared to a flat 2D system. The results demonstrate the potential of GelMA ICC scaffolds to become an effective tissue engineering platform for drug screening and regenerative medicine.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Lee, Bae Hoon
Shirahama, Hitomi
Kim, Myung Hee
Lee, Jae Ho
Cho, Nam-Joon
Tan, Lay Poh
format Article
author Lee, Bae Hoon
Shirahama, Hitomi
Kim, Myung Hee
Lee, Jae Ho
Cho, Nam-Joon
Tan, Lay Poh
author_sort Lee, Bae Hoon
title Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues
title_short Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues
title_full Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues
title_fullStr Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues
title_full_unstemmed Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues
title_sort colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues
publishDate 2018
url https://hdl.handle.net/10356/88542
http://hdl.handle.net/10220/45821
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