Cells alignment and accumulation using acoustic nozzle for 3D printing

Arrangement or patterning of microparticles/cells would enhance the efficiency, performance, and function of the printed construct. This could be utilized in various applications such as fibers reinforced polymer matrix, hydrogel scaffold, and 3D printed biological samples. Magnetic manipulation...

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Main Authors: Sriphutkiat, Yannapol, Kasetsirikul, Surasak, Ketpun, Dettachai, Zhou, Yufeng
Other Authors: School of Mechanical and Aerospace Engineering
Format: Conference or Workshop Item
Language:English
Published: 2018
Subjects:
Online Access:https://hdl.handle.net/10356/88563
http://hdl.handle.net/10220/45866
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-885632020-09-24T20:12:33Z Cells alignment and accumulation using acoustic nozzle for 3D printing Sriphutkiat, Yannapol Kasetsirikul, Surasak Ketpun, Dettachai Zhou, Yufeng School of Mechanical and Aerospace Engineering Proceedings of the 3rd International Conference on Progress in Additive Manufacturing (Pro-AM 2018) Singapore Centre for 3D Printing 3D Bioprinting DRNTU::Engineering::Mechanical engineering::Prototyping Particle/Cell Manipulation Arrangement or patterning of microparticles/cells would enhance the efficiency, performance, and function of the printed construct. This could be utilized in various applications such as fibers reinforced polymer matrix, hydrogel scaffold, and 3D printed biological samples. Magnetic manipulation and dielectrophoresis have some drawbacks, such as time-consuming and only valid for samples with specific physical properties. Here, acoustic manipulation of microparticles in the cylindrical glass nozzle is proposed to produce a structural vibration at the specific resonant frequency. With the acoustic excitation, microparticles were accumulated at the center of the nozzle and consequently printed construct at the fundamental frequency of 871 kHz. The distribution of microparticles fits well with a Gaussian distribution. In addition, C2C12 cells were also patterned by the acoustic waves inside the cylindrical glass tube and in the printed hydrogel construct. Overall, the proposed acoustic approach is able to accumulate the microparticles and biological cells in the printed construct at a low cost, easy configuration, low power, and high biocompatibility. MOE (Min. of Education, S’pore) Published version 2018-09-06T07:02:15Z 2019-12-06T17:06:11Z 2018-09-06T07:02:15Z 2019-12-06T17:06:11Z 2018 Conference Paper Sriphutkiat, Y., Kasetsirikul, S., Ketpun, D., & Zhou, Y. (2018). Cells alignment and accumulation using acoustic nozzle for 3D printing. Proceedings of the 3rd International Conference on Progress in Additive Manufacturing (Pro-AM 2018), 383-388. doi:10.25341/D46C73 https://hdl.handle.net/10356/88563 http://hdl.handle.net/10220/45866 10.25341/D46C73 en © 2018 Nanyang Technological University. Published by Nanyang Technological University, Singapore. 6 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic 3D Bioprinting
DRNTU::Engineering::Mechanical engineering::Prototyping
Particle/Cell Manipulation
spellingShingle 3D Bioprinting
DRNTU::Engineering::Mechanical engineering::Prototyping
Particle/Cell Manipulation
Sriphutkiat, Yannapol
Kasetsirikul, Surasak
Ketpun, Dettachai
Zhou, Yufeng
Cells alignment and accumulation using acoustic nozzle for 3D printing
description Arrangement or patterning of microparticles/cells would enhance the efficiency, performance, and function of the printed construct. This could be utilized in various applications such as fibers reinforced polymer matrix, hydrogel scaffold, and 3D printed biological samples. Magnetic manipulation and dielectrophoresis have some drawbacks, such as time-consuming and only valid for samples with specific physical properties. Here, acoustic manipulation of microparticles in the cylindrical glass nozzle is proposed to produce a structural vibration at the specific resonant frequency. With the acoustic excitation, microparticles were accumulated at the center of the nozzle and consequently printed construct at the fundamental frequency of 871 kHz. The distribution of microparticles fits well with a Gaussian distribution. In addition, C2C12 cells were also patterned by the acoustic waves inside the cylindrical glass tube and in the printed hydrogel construct. Overall, the proposed acoustic approach is able to accumulate the microparticles and biological cells in the printed construct at a low cost, easy configuration, low power, and high biocompatibility.
author2 School of Mechanical and Aerospace Engineering
author_facet School of Mechanical and Aerospace Engineering
Sriphutkiat, Yannapol
Kasetsirikul, Surasak
Ketpun, Dettachai
Zhou, Yufeng
format Conference or Workshop Item
author Sriphutkiat, Yannapol
Kasetsirikul, Surasak
Ketpun, Dettachai
Zhou, Yufeng
author_sort Sriphutkiat, Yannapol
title Cells alignment and accumulation using acoustic nozzle for 3D printing
title_short Cells alignment and accumulation using acoustic nozzle for 3D printing
title_full Cells alignment and accumulation using acoustic nozzle for 3D printing
title_fullStr Cells alignment and accumulation using acoustic nozzle for 3D printing
title_full_unstemmed Cells alignment and accumulation using acoustic nozzle for 3D printing
title_sort cells alignment and accumulation using acoustic nozzle for 3d printing
publishDate 2018
url https://hdl.handle.net/10356/88563
http://hdl.handle.net/10220/45866
_version_ 1681057882766835712