Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB

Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm‐control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c‐di‐GMP) is a positive regulator of biofilm f...

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Main Authors: Fazli, Mustafa, Rybtke, Morten, Steiner, Elisabeth, Weidel, Elisabeth, Berthelsen, Jens, Groizeleau, Julie, Bin, Wu, Zhi, Boo Zhao, Yaming, Zhang, Kaever, Volkhard, Givskov, Michael, Hartmann, Rolf W., Eberl, Leo, Tolker-Nielsen, Tim
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2018
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Online Access:https://hdl.handle.net/10356/88805
http://hdl.handle.net/10220/45972
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-888052023-02-28T17:02:49Z Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB Fazli, Mustafa Rybtke, Morten Steiner, Elisabeth Weidel, Elisabeth Berthelsen, Jens Groizeleau, Julie Bin, Wu Zhi, Boo Zhao Yaming, Zhang Kaever, Volkhard Givskov, Michael Hartmann, Rolf W. Eberl, Leo Tolker-Nielsen, Tim School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering C-di-GMP Biofilm DRNTU::Science::Biological sciences Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm‐control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c‐di‐GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c‐di‐GMP effectors is needed. We provide evidence that c‐di‐GMP, the alternative sigma factor RpoN (σ54), and the enhancer‐binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm‐stabilizing exopolysaccharide. Our findings suggest that BerB binds c‐di‐GMP, and activates RpoN‐dependent transcription of the berA gene coding for a c‐di‐GMP‐responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm‐stabilizing exopolysaccharide in response to high c‐di‐GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c‐di‐GMP. This type of regulation may allow tight control of the expenditure of cellular resources. Published version 2018-09-12T08:44:38Z 2019-12-06T17:11:13Z 2018-09-12T08:44:38Z 2019-12-06T17:11:13Z 2017 Journal Article Fazli, M., Rybtke, M., Steiner, E., Weidel, E., Berthelsen, J., Groizeleau, J., . . . Tolker-Nielsen, T. (2017). Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB. MicrobiologyOpen, 6(4), e00480-. doi:10.1002/mbo3.480 https://hdl.handle.net/10356/88805 http://hdl.handle.net/10220/45972 10.1002/mbo3.480 en MicrobiologyOpen © 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. 13 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic C-di-GMP
Biofilm
DRNTU::Science::Biological sciences
spellingShingle C-di-GMP
Biofilm
DRNTU::Science::Biological sciences
Fazli, Mustafa
Rybtke, Morten
Steiner, Elisabeth
Weidel, Elisabeth
Berthelsen, Jens
Groizeleau, Julie
Bin, Wu
Zhi, Boo Zhao
Yaming, Zhang
Kaever, Volkhard
Givskov, Michael
Hartmann, Rolf W.
Eberl, Leo
Tolker-Nielsen, Tim
Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB
description Knowledge about the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm‐control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c‐di‐GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c‐di‐GMP effectors is needed. We provide evidence that c‐di‐GMP, the alternative sigma factor RpoN (σ54), and the enhancer‐binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm‐stabilizing exopolysaccharide. Our findings suggest that BerB binds c‐di‐GMP, and activates RpoN‐dependent transcription of the berA gene coding for a c‐di‐GMP‐responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm‐stabilizing exopolysaccharide in response to high c‐di‐GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c‐di‐GMP. This type of regulation may allow tight control of the expenditure of cellular resources.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Fazli, Mustafa
Rybtke, Morten
Steiner, Elisabeth
Weidel, Elisabeth
Berthelsen, Jens
Groizeleau, Julie
Bin, Wu
Zhi, Boo Zhao
Yaming, Zhang
Kaever, Volkhard
Givskov, Michael
Hartmann, Rolf W.
Eberl, Leo
Tolker-Nielsen, Tim
format Article
author Fazli, Mustafa
Rybtke, Morten
Steiner, Elisabeth
Weidel, Elisabeth
Berthelsen, Jens
Groizeleau, Julie
Bin, Wu
Zhi, Boo Zhao
Yaming, Zhang
Kaever, Volkhard
Givskov, Michael
Hartmann, Rolf W.
Eberl, Leo
Tolker-Nielsen, Tim
author_sort Fazli, Mustafa
title Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB
title_short Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB
title_full Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB
title_fullStr Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB
title_full_unstemmed Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB
title_sort regulation of burkholderia cenocepacia biofilm formation by rpon and the c-di-gmp effector berb
publishDate 2018
url https://hdl.handle.net/10356/88805
http://hdl.handle.net/10220/45972
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