The metagenomics analysis of air, dust and surfaces of tropical buildings

• Main Authors: Lau, Kenny Jia Xu, Uchida, Akira, Luhung, Irvan, Lim, Serene Boon Yuean, Schuster, Stephan Christoph
• Other Authors: Interdisciplinary Graduate School (IGS)
• Format: Conference or Workshop Item
• Language: English
• Published: 2019
• Subjects: Surface Swabbing; Low Biomass Metagenomics; Science::Chemistry
• Online Access: https://hdl.handle.net/10356/89002; http://hdl.handle.net/10220/49610; http://www.indoorair2018.org/
• Institution: Nanyang Technological University

This study focuses on systematic comparisons between 3 types of samples (dust, air, surface) commonly analyzed in environmental quality studies for better understanding on the source-sink relationships among microbial communities found in different samples. Dust, air and surface swab samples were collected from an indoor and its nearby outdoor locations. DNA was then extracted from the samples and direct (shotgun) metagenomic sequencing approach was applied for microbial community profiling of the samples. The current results indicate a moderate to high correlation between the surface and the settling dust samples.