The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger

The slow and promiscuous properties of the CO2-fixing enzyme Rubisco constrain photosynthetic efficiency and have prompted the evolution of powerful CO2 concentrating mechanisms (CCMs). In eukaryotic microalgae a key strategy involves sequestration of the enzyme in the pyrenoid, a liquid non-membran...

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Main Authors: Wunder, Tobias, Cheng, Steven Le Hung, Lai, Soak-Kuan, Li, Hoi-Yeung, Mueller-Cajar, Oliver
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2019
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Online Access:https://hdl.handle.net/10356/89125
http://hdl.handle.net/10220/47672
https://doi.org/10.21979/N9/RM7TRL
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-891252023-02-28T17:03:24Z The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger Wunder, Tobias Cheng, Steven Le Hung Lai, Soak-Kuan Li, Hoi-Yeung Mueller-Cajar, Oliver School of Biological Sciences DRNTU::Science::Biological sciences Rubisco Essential Pyrenoid Component 1 The slow and promiscuous properties of the CO2-fixing enzyme Rubisco constrain photosynthetic efficiency and have prompted the evolution of powerful CO2 concentrating mechanisms (CCMs). In eukaryotic microalgae a key strategy involves sequestration of the enzyme in the pyrenoid, a liquid non-membranous compartment of the chloroplast stroma. Here we show using pure components that two proteins, Rubisco and the linker protein Essential Pyrenoid Component 1 (EPYC1), are both necessary and sufficient to phase separate and form liquid droplets. The phase-separated Rubisco is functional. Droplet composition is dynamic and components rapidly exchange with the bulk solution. Heterologous and chimeric Rubiscos exhibit variability in their tendency to demix with EPYC1. The ability to dissect aspects of pyrenoid biochemistry in vitro will permit us to inform and guide synthetic biology ambitions aiming to engineer microalgal CCMs into crop plants. MOE (Min. of Education, S’pore) Published version 2019-02-15T02:02:14Z 2019-12-06T17:18:26Z 2019-02-15T02:02:14Z 2019-12-06T17:18:26Z 2018 Journal Article Wunder, T., Cheng, S. L. H., Lai, S.-K., Li, H.-Y., & Mueller-Cajar, O. (2018). The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger. Nature Communications, 9(1), 5076-. doi:10.1038/s41467-018-07624-w https://hdl.handle.net/10356/89125 http://hdl.handle.net/10220/47672 10.1038/s41467-018-07624-w en Nature Communications https://doi.org/10.21979/N9/RM7TRL © 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 10 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
Rubisco
Essential Pyrenoid Component 1
spellingShingle DRNTU::Science::Biological sciences
Rubisco
Essential Pyrenoid Component 1
Wunder, Tobias
Cheng, Steven Le Hung
Lai, Soak-Kuan
Li, Hoi-Yeung
Mueller-Cajar, Oliver
The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger
description The slow and promiscuous properties of the CO2-fixing enzyme Rubisco constrain photosynthetic efficiency and have prompted the evolution of powerful CO2 concentrating mechanisms (CCMs). In eukaryotic microalgae a key strategy involves sequestration of the enzyme in the pyrenoid, a liquid non-membranous compartment of the chloroplast stroma. Here we show using pure components that two proteins, Rubisco and the linker protein Essential Pyrenoid Component 1 (EPYC1), are both necessary and sufficient to phase separate and form liquid droplets. The phase-separated Rubisco is functional. Droplet composition is dynamic and components rapidly exchange with the bulk solution. Heterologous and chimeric Rubiscos exhibit variability in their tendency to demix with EPYC1. The ability to dissect aspects of pyrenoid biochemistry in vitro will permit us to inform and guide synthetic biology ambitions aiming to engineer microalgal CCMs into crop plants.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Wunder, Tobias
Cheng, Steven Le Hung
Lai, Soak-Kuan
Li, Hoi-Yeung
Mueller-Cajar, Oliver
format Article
author Wunder, Tobias
Cheng, Steven Le Hung
Lai, Soak-Kuan
Li, Hoi-Yeung
Mueller-Cajar, Oliver
author_sort Wunder, Tobias
title The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger
title_short The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger
title_full The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger
title_fullStr The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger
title_full_unstemmed The phase separation underlying the pyrenoid-based microalgal Rubisco supercharger
title_sort phase separation underlying the pyrenoid-based microalgal rubisco supercharger
publishDate 2019
url https://hdl.handle.net/10356/89125
http://hdl.handle.net/10220/47672
https://doi.org/10.21979/N9/RM7TRL
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