Selective effects of miRNA effector protein Argonaute on miRNA processing
Since the discovery of miRNAs, studies have been focused on understanding the mechanisms of miRNA biogenesis. However, the molecular/mechanistic function of some miRNA biogenesis factors remain elusive. This project examines a novel role for Argonaute proteins as a factor in regulation of the pre-mi...
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Nanyang Technological University
2018
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sg-ntu-dr.10356-895472023-02-28T18:38:50Z Selective effects of miRNA effector protein Argonaute on miRNA processing Lee, Li Ming Katsutomo Okamura School of Biological Sciences Temasek Laboratories DRNTU::Science::Biological sciences::Molecular biology Since the discovery of miRNAs, studies have been focused on understanding the mechanisms of miRNA biogenesis. However, the molecular/mechanistic function of some miRNA biogenesis factors remain elusive. This project examines a novel role for Argonaute proteins as a factor in regulation of the pre-mir-124 hairpin. Pre-miR-124, but not other pre-miRNAs, was reduced in response to Ago overexpression. This phenomenon was reproducible in mouse and fly cells, suggesting that the mechanism is broadly conserved. Ago-dependent decrease of pre-miR-124 was shown not to be caused by secondary effects of miR-124 target regulation. Experiments using Dicer knockout cells revealed that Ago-induced reduction of pre-mir-124 is a Dicer-dependent phenomenon. Overexpression of Ago2 appeared to result in an increase in the average neurite length of N2a cells. These observations suggest previously unknown roles for the core miRNA biogenesis factors. Doctor of Philosophy 2018-10-15T02:33:42Z 2019-12-06T17:28:06Z 2018-10-15T02:33:42Z 2019-12-06T17:28:06Z 2018 Thesis-Doctor of Philosophy Lee, L. M. (2018). Selective effects of miRNA effector protein Argonaute on miRNA processing. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/89547 http://hdl.handle.net/10220/46311 10.32657/10220/46311 en 165 p. application/pdf Nanyang Technological University |
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DRNTU::Science::Biological sciences::Molecular biology Lee, Li Ming Selective effects of miRNA effector protein Argonaute on miRNA processing |
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Since the discovery of miRNAs, studies have been focused on understanding the mechanisms of miRNA biogenesis. However, the molecular/mechanistic function of some miRNA biogenesis factors remain elusive. This project examines a novel role for Argonaute proteins as a factor in regulation of the pre-mir-124 hairpin. Pre-miR-124, but not other pre-miRNAs, was reduced in response to Ago overexpression. This phenomenon was reproducible in mouse and fly cells, suggesting that the mechanism is broadly conserved. Ago-dependent decrease of pre-miR-124 was shown not to be caused by secondary effects of miR-124 target regulation. Experiments using Dicer knockout cells revealed that Ago-induced reduction of pre-mir-124 is a Dicer-dependent phenomenon. Overexpression of Ago2 appeared to result in an increase in the average neurite length of N2a cells. These observations suggest previously unknown roles for the core miRNA biogenesis factors. |
author2 |
Katsutomo Okamura |
author_facet |
Katsutomo Okamura Lee, Li Ming |
format |
Thesis-Doctor of Philosophy |
author |
Lee, Li Ming |
author_sort |
Lee, Li Ming |
title |
Selective effects of miRNA effector protein Argonaute on miRNA processing |
title_short |
Selective effects of miRNA effector protein Argonaute on miRNA processing |
title_full |
Selective effects of miRNA effector protein Argonaute on miRNA processing |
title_fullStr |
Selective effects of miRNA effector protein Argonaute on miRNA processing |
title_full_unstemmed |
Selective effects of miRNA effector protein Argonaute on miRNA processing |
title_sort |
selective effects of mirna effector protein argonaute on mirna processing |
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Nanyang Technological University |
publishDate |
2018 |
url |
https://hdl.handle.net/10356/89547 http://hdl.handle.net/10220/46311 |
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1759855042882437120 |