Epithelial-mesenchymal transition of cancer cells using bioengineered hybrid scaffold composed of hydrogel/3D-fibrous framework

Cancer cells undergoing epithelial-mesenchymal transition (EMT) acquire stem cell-like phenotype associated with malignant behaviour, chemoresistance, and relapse. Current two-dimensional (2D) in-vitro culture models of tumorigenesis are inadequate to replicate the complexity of in-vivo microenviron...

Full description

Saved in:
Bibliographic Details
Main Authors: Pal, Mintu, Chen, Huizhi, Lee, Bae Hoon, Lee, Justin Yin Hao, Yip, Yun Sheng, Tan, Nguan Soon, Tan, Lay Poh
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2019
Subjects:
Online Access:https://hdl.handle.net/10356/89858
http://hdl.handle.net/10220/49325
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-89858
record_format dspace
spelling sg-ntu-dr.10356-898582023-07-14T15:45:58Z Epithelial-mesenchymal transition of cancer cells using bioengineered hybrid scaffold composed of hydrogel/3D-fibrous framework Pal, Mintu Chen, Huizhi Lee, Bae Hoon Lee, Justin Yin Hao Yip, Yun Sheng Tan, Nguan Soon Tan, Lay Poh School of Materials Science & Engineering School of Biological Sciences Lee Kong Chian School of Medicine (LKCMedicine) Biomaterials–cells Science::Biological sciences Cancer Models Cancer cells undergoing epithelial-mesenchymal transition (EMT) acquire stem cell-like phenotype associated with malignant behaviour, chemoresistance, and relapse. Current two-dimensional (2D) in-vitro culture models of tumorigenesis are inadequate to replicate the complexity of in-vivo microenvironment. Therefore, the generation of functional three-dimensional (3D) constructs is a fundamental prerequisite to form multi-cellular tumour spheroids for studying basic pathological mechanisms. In this study, we focused on two major points (i) designing and fabrication of 3D hybrid scaffolds comprising electrospun fibers with cancer cells embedded within hydrogels, and (ii) determining the potential roles of 3D hybrid scaffolds associated with EMT in cancer progression and metastasis. Our findings revealed that 3D hybrid scaffold enhances cell proliferation and induces cancer cells to undergo EMT, as demonstrated by significant up-regulation of EMT associated transcriptional factors including Snail1, Zeb1, and Twist2; and mesenchymal markers whereas epithelial marker, E-Cadherin was downregulated. Remarkably, this induction is independent of cancer cell-type as similar results were obtained for breast cancer cells, MDA-MB-231 and gastric cancer cells, MKN74. Moreover, the hybrid scaffolds enrich aggressive cancer cells with stem cell properties. We showed that our 3D scaffolds could trigger EMT of cancer cells which could provide a useful model for studying anticancer therapeutics against metastasis. MOE (Min. of Education, S’pore) Published version 2019-07-12T04:43:44Z 2019-12-06T17:35:12Z 2019-07-12T04:43:44Z 2019-12-06T17:35:12Z 2019 Journal Article Pal, M., Chen, H., Lee, B. H., Lee, J. Y. H., Yip, Y. S., Tan, N. S., & Tan, L. P. (2019). Epithelial-mesenchymal transition of cancer cells using bioengineered hybrid scaffold composed of hydrogel/3D-fibrous framework. Scientific Reports, 9(1), 8997-. doi:10.1038/s41598-019-45384-9 https://hdl.handle.net/10356/89858 http://hdl.handle.net/10220/49325 10.1038/s41598-019-45384-9 en Scientific Reports © 2019 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 11 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Biomaterials–cells
Science::Biological sciences
Cancer Models
spellingShingle Biomaterials–cells
Science::Biological sciences
Cancer Models
Pal, Mintu
Chen, Huizhi
Lee, Bae Hoon
Lee, Justin Yin Hao
Yip, Yun Sheng
Tan, Nguan Soon
Tan, Lay Poh
Epithelial-mesenchymal transition of cancer cells using bioengineered hybrid scaffold composed of hydrogel/3D-fibrous framework
description Cancer cells undergoing epithelial-mesenchymal transition (EMT) acquire stem cell-like phenotype associated with malignant behaviour, chemoresistance, and relapse. Current two-dimensional (2D) in-vitro culture models of tumorigenesis are inadequate to replicate the complexity of in-vivo microenvironment. Therefore, the generation of functional three-dimensional (3D) constructs is a fundamental prerequisite to form multi-cellular tumour spheroids for studying basic pathological mechanisms. In this study, we focused on two major points (i) designing and fabrication of 3D hybrid scaffolds comprising electrospun fibers with cancer cells embedded within hydrogels, and (ii) determining the potential roles of 3D hybrid scaffolds associated with EMT in cancer progression and metastasis. Our findings revealed that 3D hybrid scaffold enhances cell proliferation and induces cancer cells to undergo EMT, as demonstrated by significant up-regulation of EMT associated transcriptional factors including Snail1, Zeb1, and Twist2; and mesenchymal markers whereas epithelial marker, E-Cadherin was downregulated. Remarkably, this induction is independent of cancer cell-type as similar results were obtained for breast cancer cells, MDA-MB-231 and gastric cancer cells, MKN74. Moreover, the hybrid scaffolds enrich aggressive cancer cells with stem cell properties. We showed that our 3D scaffolds could trigger EMT of cancer cells which could provide a useful model for studying anticancer therapeutics against metastasis.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Pal, Mintu
Chen, Huizhi
Lee, Bae Hoon
Lee, Justin Yin Hao
Yip, Yun Sheng
Tan, Nguan Soon
Tan, Lay Poh
format Article
author Pal, Mintu
Chen, Huizhi
Lee, Bae Hoon
Lee, Justin Yin Hao
Yip, Yun Sheng
Tan, Nguan Soon
Tan, Lay Poh
author_sort Pal, Mintu
title Epithelial-mesenchymal transition of cancer cells using bioengineered hybrid scaffold composed of hydrogel/3D-fibrous framework
title_short Epithelial-mesenchymal transition of cancer cells using bioengineered hybrid scaffold composed of hydrogel/3D-fibrous framework
title_full Epithelial-mesenchymal transition of cancer cells using bioengineered hybrid scaffold composed of hydrogel/3D-fibrous framework
title_fullStr Epithelial-mesenchymal transition of cancer cells using bioengineered hybrid scaffold composed of hydrogel/3D-fibrous framework
title_full_unstemmed Epithelial-mesenchymal transition of cancer cells using bioengineered hybrid scaffold composed of hydrogel/3D-fibrous framework
title_sort epithelial-mesenchymal transition of cancer cells using bioengineered hybrid scaffold composed of hydrogel/3d-fibrous framework
publishDate 2019
url https://hdl.handle.net/10356/89858
http://hdl.handle.net/10220/49325
_version_ 1772828546766471168