Cellular resolution corneal imaging with extended imaging range

Current optical coherence tomography (OCT) technology, which is used for imaging the eye’s anterior segment, has been established as a clinical gold standard for the diagnosis of corneal diseases. However, the cellular resolution level information that is critical for many clinical applications is s...

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Main Authors: Wang, Lulu, Xiong, Qiaozhou, Ge, Xin, Bo, En, Xie, Jun, Liu, Xinyu, Yu, Xiaojun, Wang, Xianghong, Wang, Nanshuo, Chen, Si, Wu, Xuan, Liu, Linbo
Other Authors: School of Electrical and Electronic Engineering
Format: Article
Language:English
Published: 2019
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Online Access:https://hdl.handle.net/10356/89907
http://hdl.handle.net/10220/47750
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-899072020-03-07T14:02:37Z Cellular resolution corneal imaging with extended imaging range Wang, Lulu Xiong, Qiaozhou Ge, Xin Bo, En Xie, Jun Liu, Xinyu Yu, Xiaojun Wang, Xianghong Wang, Nanshuo Chen, Si Wu, Xuan Liu, Linbo School of Electrical and Electronic Engineering Digital Adaptive Optics Optical Coherence Tomography DRNTU::Engineering::Electrical and electronic engineering Current optical coherence tomography (OCT) technology, which is used for imaging the eye’s anterior segment, has been established as a clinical gold standard for the diagnosis of corneal diseases. However, the cellular resolution level information that is critical for many clinical applications is still not available. The major technical challenges toward cellular resolution OCT imaging are the limited ranging depth and depth of focus (DOF). In this work, we present a novel ultrahigh resolution OCT system that achieves an isotropic spatial resolution of <2 µm in tissue. The proposed system could approximately double the ranging depth and extend the DOF using the dual-spectrometer design and the forward-model based digital refocusing method, respectively. We demonstrate that the novel system is capable of visualizing the full thickness of the pig cornea over the ranging depth of 3.5 mm and the border of the corneal endothelial cells 8 times Rayleigh range away from the focal plane. This technology has the potential to realize cellular resolution corneal imaging in vivo. NRF (Natl Research Foundation, S’pore) ASTAR (Agency for Sci., Tech. and Research, S’pore) MOE (Min. of Education, S’pore) Published version 2019-03-01T08:31:42Z 2019-12-06T17:36:20Z 2019-03-01T08:31:42Z 2019-12-06T17:36:20Z 2019 Journal Article Wang, L., Xiong, Q., Ge, X., Bo, E., Xie, J., Liu, X., . . . Liu, L. (2019). Cellular resolution corneal imaging with extended imaging range. Optics Express, 27(2), 1298-. doi:10.1364/OE.27.001298 https://hdl.handle.net/10356/89907 http://hdl.handle.net/10220/47750 10.1364/OE.27.001298 en Optics Express © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. 12 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Digital Adaptive Optics
Optical Coherence Tomography
DRNTU::Engineering::Electrical and electronic engineering
spellingShingle Digital Adaptive Optics
Optical Coherence Tomography
DRNTU::Engineering::Electrical and electronic engineering
Wang, Lulu
Xiong, Qiaozhou
Ge, Xin
Bo, En
Xie, Jun
Liu, Xinyu
Yu, Xiaojun
Wang, Xianghong
Wang, Nanshuo
Chen, Si
Wu, Xuan
Liu, Linbo
Cellular resolution corneal imaging with extended imaging range
description Current optical coherence tomography (OCT) technology, which is used for imaging the eye’s anterior segment, has been established as a clinical gold standard for the diagnosis of corneal diseases. However, the cellular resolution level information that is critical for many clinical applications is still not available. The major technical challenges toward cellular resolution OCT imaging are the limited ranging depth and depth of focus (DOF). In this work, we present a novel ultrahigh resolution OCT system that achieves an isotropic spatial resolution of <2 µm in tissue. The proposed system could approximately double the ranging depth and extend the DOF using the dual-spectrometer design and the forward-model based digital refocusing method, respectively. We demonstrate that the novel system is capable of visualizing the full thickness of the pig cornea over the ranging depth of 3.5 mm and the border of the corneal endothelial cells 8 times Rayleigh range away from the focal plane. This technology has the potential to realize cellular resolution corneal imaging in vivo.
author2 School of Electrical and Electronic Engineering
author_facet School of Electrical and Electronic Engineering
Wang, Lulu
Xiong, Qiaozhou
Ge, Xin
Bo, En
Xie, Jun
Liu, Xinyu
Yu, Xiaojun
Wang, Xianghong
Wang, Nanshuo
Chen, Si
Wu, Xuan
Liu, Linbo
format Article
author Wang, Lulu
Xiong, Qiaozhou
Ge, Xin
Bo, En
Xie, Jun
Liu, Xinyu
Yu, Xiaojun
Wang, Xianghong
Wang, Nanshuo
Chen, Si
Wu, Xuan
Liu, Linbo
author_sort Wang, Lulu
title Cellular resolution corneal imaging with extended imaging range
title_short Cellular resolution corneal imaging with extended imaging range
title_full Cellular resolution corneal imaging with extended imaging range
title_fullStr Cellular resolution corneal imaging with extended imaging range
title_full_unstemmed Cellular resolution corneal imaging with extended imaging range
title_sort cellular resolution corneal imaging with extended imaging range
publishDate 2019
url https://hdl.handle.net/10356/89907
http://hdl.handle.net/10220/47750
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