Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes

With the aim of discovering contribution of histone H1 to linking number changes of DNA, determination of linking number differences between histone H1-free circular polynucleosomes and histone H1-bound circular polynucleosomes was carried out during our investigations. Our results showed that on av...

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Main Authors: Zhang, Hao, Li, Tianhu
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2019
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Online Access:https://hdl.handle.net/10356/90151
http://hdl.handle.net/10220/48431
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-901512020-03-07T11:43:39Z Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes Zhang, Hao Li, Tianhu School of Physical and Mathematical Sciences Nucleosome Histone Octamer DRNTU::Science::Chemistry With the aim of discovering contribution of histone H1 to linking number changes of DNA, determination of linking number differences between histone H1-free circular polynucleosomes and histone H1-bound circular polynucleosomes was carried out during our investigations. Our results showed that on average, binding of ∼11.5 histone H1 molecules causes one linking number change in circular polynucleosomes in the presence of 1.5 mM spermidine. When concentrations of spermidine decreases or increases, these linking number differences decrease significantly. It is therefore evident that linking number changes caused by histone H1 are spermidine concentration-dependent. MOE (Min. of Education, S’pore) Accepted version 2019-05-29T02:04:24Z 2019-12-06T17:41:53Z 2019-05-29T02:04:24Z 2019-12-06T17:41:53Z 2018 Journal Article Zhang, H., & Li, T. (2018). Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes. Bioorganic & Medicinal Chemistry Letters, 28(3), 537-540. doi:10.1016/j.bmcl.2017.10.072 0960-894X https://hdl.handle.net/10356/90151 http://hdl.handle.net/10220/48431 10.1016/j.bmcl.2017.10.072 en Bioorganic & Medicinal Chemistry Letters © 2017 Elsevier Ltd.. All rights reserved. This paper was published in Bioorganic & Medicinal Chemistry Letters and is made available with permission of Elsevier Ltd. 5 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Nucleosome
Histone Octamer
DRNTU::Science::Chemistry
spellingShingle Nucleosome
Histone Octamer
DRNTU::Science::Chemistry
Zhang, Hao
Li, Tianhu
Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes
description With the aim of discovering contribution of histone H1 to linking number changes of DNA, determination of linking number differences between histone H1-free circular polynucleosomes and histone H1-bound circular polynucleosomes was carried out during our investigations. Our results showed that on average, binding of ∼11.5 histone H1 molecules causes one linking number change in circular polynucleosomes in the presence of 1.5 mM spermidine. When concentrations of spermidine decreases or increases, these linking number differences decrease significantly. It is therefore evident that linking number changes caused by histone H1 are spermidine concentration-dependent.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Zhang, Hao
Li, Tianhu
format Article
author Zhang, Hao
Li, Tianhu
author_sort Zhang, Hao
title Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes
title_short Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes
title_full Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes
title_fullStr Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes
title_full_unstemmed Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes
title_sort quantitative determination of linking number differences between circular polynucleosomes and histone h1-bound circular polynucleosomes
publishDate 2019
url https://hdl.handle.net/10356/90151
http://hdl.handle.net/10220/48431
_version_ 1681041012413169664