Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae)

Crassulacean acid metabolism (CAM) photosynthesis is a modification of the core C3 photosynthetic pathway that improves the ability of plants to assimilate carbon in water-limited environments. CAM plants fix CO2 mostly at night, when transpiration rates are low. All of the CAM pathway genes exist i...

Full description

Saved in:
Bibliographic Details
Main Authors: Winter, Klaus, Leebens-Mack, Jim, Heyduk, Karolina, Hwang, Michelle, Albert, Victor, Silvera, Katia, Lan, Tianying, Farr, Kimberly, Chang, Tien-Hao, Chan, Ming-Tsair
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2019
Subjects:
Online Access:https://hdl.handle.net/10356/90254
http://hdl.handle.net/10220/48461
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-90254
record_format dspace
spelling sg-ntu-dr.10356-902542023-02-28T17:03:10Z Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae) Winter, Klaus Leebens-Mack, Jim Heyduk, Karolina Hwang, Michelle Albert, Victor Silvera, Katia Lan, Tianying Farr, Kimberly Chang, Tien-Hao Chan, Ming-Tsair School of Biological Sciences RNA-seq Transcriptomics DRNTU::Science::Biological sciences Crassulacean acid metabolism (CAM) photosynthesis is a modification of the core C3 photosynthetic pathway that improves the ability of plants to assimilate carbon in water-limited environments. CAM plants fix CO2 mostly at night, when transpiration rates are low. All of the CAM pathway genes exist in ancestral C3 species, but the timing and magnitude of expression are greatly altered between C3 and CAM species. Understanding these regulatory changes is key to elucidating the mechanism by which CAM evolved from C3. Here, we use two closely related species in the Orchidaceae, Erycina pusilla (CAM) and Erycina crista-galli (C3), to conduct comparative transcriptomic analyses across multiple time points. Clustering of genes with expression variation across the diel cycle revealed some canonical CAM pathway genes similarly expressed in both species, regardless of photosynthetic pathway. However, gene network construction indicated that 149 gene families had significant differences in network connectivity and were further explored for these functional enrichments. Genes involved in light sensing and ABA signaling were some of the most differently connected genes between the C3 and CAM Erycina species, in agreement with the contrasting diel patterns of stomatal conductance in C3 and CAM plants. Our results suggest changes to transcriptional cascades are important for the transition from C3 to CAM photosynthesis in Erycina. Published version 2019-05-29T08:35:55Z 2019-12-06T17:44:07Z 2019-05-29T08:35:55Z 2019-12-06T17:44:07Z 2019 Journal Article Heyduk, K., Hwang, M., Albert, V., Silvera, K., Lan, T., Farr, K., . . . Leebens-Mack, J. (2019). Altered Gene Regulatory Networks Are Associated With the Transition From C3 to Crassulacean Acid Metabolism in Erycina (Oncidiinae: Orchidaceae). Frontiers in Plant Science, 9, 2000-. doi:10.3389/fpls.2018.02000 https://hdl.handle.net/10356/90254 http://hdl.handle.net/10220/48461 10.3389/fpls.2018.02000 en Frontiers in Plant Science © 2019 Heyduk, Hwang, Albert, Silvera, Lan, Farr, Chang, Chan, Winter and Leebens-Mack. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 15 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic RNA-seq
Transcriptomics
DRNTU::Science::Biological sciences
spellingShingle RNA-seq
Transcriptomics
DRNTU::Science::Biological sciences
Winter, Klaus
Leebens-Mack, Jim
Heyduk, Karolina
Hwang, Michelle
Albert, Victor
Silvera, Katia
Lan, Tianying
Farr, Kimberly
Chang, Tien-Hao
Chan, Ming-Tsair
Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae)
description Crassulacean acid metabolism (CAM) photosynthesis is a modification of the core C3 photosynthetic pathway that improves the ability of plants to assimilate carbon in water-limited environments. CAM plants fix CO2 mostly at night, when transpiration rates are low. All of the CAM pathway genes exist in ancestral C3 species, but the timing and magnitude of expression are greatly altered between C3 and CAM species. Understanding these regulatory changes is key to elucidating the mechanism by which CAM evolved from C3. Here, we use two closely related species in the Orchidaceae, Erycina pusilla (CAM) and Erycina crista-galli (C3), to conduct comparative transcriptomic analyses across multiple time points. Clustering of genes with expression variation across the diel cycle revealed some canonical CAM pathway genes similarly expressed in both species, regardless of photosynthetic pathway. However, gene network construction indicated that 149 gene families had significant differences in network connectivity and were further explored for these functional enrichments. Genes involved in light sensing and ABA signaling were some of the most differently connected genes between the C3 and CAM Erycina species, in agreement with the contrasting diel patterns of stomatal conductance in C3 and CAM plants. Our results suggest changes to transcriptional cascades are important for the transition from C3 to CAM photosynthesis in Erycina.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Winter, Klaus
Leebens-Mack, Jim
Heyduk, Karolina
Hwang, Michelle
Albert, Victor
Silvera, Katia
Lan, Tianying
Farr, Kimberly
Chang, Tien-Hao
Chan, Ming-Tsair
format Article
author Winter, Klaus
Leebens-Mack, Jim
Heyduk, Karolina
Hwang, Michelle
Albert, Victor
Silvera, Katia
Lan, Tianying
Farr, Kimberly
Chang, Tien-Hao
Chan, Ming-Tsair
author_sort Winter, Klaus
title Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae)
title_short Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae)
title_full Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae)
title_fullStr Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae)
title_full_unstemmed Altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae)
title_sort altered gene regulatory networks are associated with the transition from c3 to crassulacean acid metabolism in erycina (oncidiinae: orchidaceae)
publishDate 2019
url https://hdl.handle.net/10356/90254
http://hdl.handle.net/10220/48461
_version_ 1759856451336011776