Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application

Two-photon fluorescence (TPE) and second harmonic generation (SHG) can been used to extract biological information from tissues at the molecular level, which is blind to traditional microscopes. Through these two image contrast mechanisms, a nonlinear laser scanning endoscope (NLSE) is able to image...

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Main Authors: Lim, Ken Choong, Li, Hao, Liu, Jiayun, Seck, Hon Luen, Yu, Xia, Kok, Shaw Wei, Zhang, Ying
Other Authors: Suter, Melissa J.
Format: Conference or Workshop Item
Language:English
Published: 2015
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Online Access:https://hdl.handle.net/10356/93529
http://hdl.handle.net/10220/25591
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-935292020-09-26T22:09:50Z Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application Lim, Ken Choong Li, Hao Liu, Jiayun Seck, Hon Luen Yu, Xia Kok, Shaw Wei Zhang, Ying Suter, Melissa J. Lam, Stephen Brenner, Matthew Tearney, Guillermo J. Wang, Thomas D. Endoscopic Microscopy X; and Optical Techniques in Pulmonary Medicine II A*STAR SIMTech DRNTU::Science::Biological sciences::Microbiology Two-photon fluorescence (TPE) and second harmonic generation (SHG) can been used to extract biological information from tissues at the molecular level, which is blind to traditional microscopes. Through these two image contrast mechanisms, a nonlinear laser scanning endoscope (NLSE) is able to image tissue cells and the extra cellular matrix (ECM) through a special fiber and miniaturized scanner without the requirement of poisonous chemical staining. Therefore, NLSE reserves high potential for in-vivo pathological study and disease diagnosis. However, the high cost and bulky size of a NLSE system has become one of the major issues preventing this technology from practical clinical operation. In this paper, we report a fiber laser based multi-modality NLSE system with compact size and low cost, ideal for in-vivo applications in clinical environments. The demonstration of the developed NLSE nonlinear imaging capability on different bio-structures in liver, retina and skin are also presented. Published version 2015-05-18T08:48:26Z 2019-12-06T18:40:56Z 2015-05-18T08:48:26Z 2019-12-06T18:40:56Z 2015 2015 Conference Paper Liu, J., Lim, K. C., Li, H., Seck, H. L., Yu, X., Kok, S. W., et al. (2015). Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 9304, 93041K-. https://hdl.handle.net/10356/93529 http://hdl.handle.net/10220/25591 10.1117/12.2078809 en © 2015 Society of Photo-optical Instrumentation Engineers. This paper was published in Progress in Biomedical Optics and Imaging - Proceedings of SPIE and is made available as an electronic reprint (preprint) with permission of Society of Photo-optical Instrumentation Engineers. The paper can be found at the following official DOI: [http://dx.doi.org/10.1117/12.2078809]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 6 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology
spellingShingle DRNTU::Science::Biological sciences::Microbiology
Lim, Ken Choong
Li, Hao
Liu, Jiayun
Seck, Hon Luen
Yu, Xia
Kok, Shaw Wei
Zhang, Ying
Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application
description Two-photon fluorescence (TPE) and second harmonic generation (SHG) can been used to extract biological information from tissues at the molecular level, which is blind to traditional microscopes. Through these two image contrast mechanisms, a nonlinear laser scanning endoscope (NLSE) is able to image tissue cells and the extra cellular matrix (ECM) through a special fiber and miniaturized scanner without the requirement of poisonous chemical staining. Therefore, NLSE reserves high potential for in-vivo pathological study and disease diagnosis. However, the high cost and bulky size of a NLSE system has become one of the major issues preventing this technology from practical clinical operation. In this paper, we report a fiber laser based multi-modality NLSE system with compact size and low cost, ideal for in-vivo applications in clinical environments. The demonstration of the developed NLSE nonlinear imaging capability on different bio-structures in liver, retina and skin are also presented.
author2 Suter, Melissa J.
author_facet Suter, Melissa J.
Lim, Ken Choong
Li, Hao
Liu, Jiayun
Seck, Hon Luen
Yu, Xia
Kok, Shaw Wei
Zhang, Ying
format Conference or Workshop Item
author Lim, Ken Choong
Li, Hao
Liu, Jiayun
Seck, Hon Luen
Yu, Xia
Kok, Shaw Wei
Zhang, Ying
author_sort Lim, Ken Choong
title Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application
title_short Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application
title_full Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application
title_fullStr Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application
title_full_unstemmed Low cost and compact nonlinear (SHG/TPE) laser scanning endoscope for bio-medical application
title_sort low cost and compact nonlinear (shg/tpe) laser scanning endoscope for bio-medical application
publishDate 2015
url https://hdl.handle.net/10356/93529
http://hdl.handle.net/10220/25591
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