Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition
We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
2011
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/93770 http://hdl.handle.net/10220/6883 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Nanyang Technological University |
| Language: | English |
| id |
sg-ntu-dr.10356-93770 |
|---|---|
| record_format |
dspace |
| spelling |
sg-ntu-dr.10356-937702023-07-14T15:50:16Z Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition Lei, Y. Cai, X. P. Li, Chang Ming Dong, Zhili Huang, W. Wang, J. X. Wei, A. Sun, Xiaowei School of Materials Science & Engineering DRNTU::Engineering::Materials::Biomaterials We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction. At an applied potential of +0.8 V versus Ag/AgCl reference electrode, ZnO nanorods based biosensor presented a high and reproducible sensitivity of 23.1 μA cm−2 mM−1 with a response time of less than 5 s. The biosensor shows a linear range from 0.01 to 3.45 mM and an experiment limit of detection of 0.01 mM. An apparent Michaelis-Menten constant of 2.9 mM shows a high affinity between glucose and GOx immobilized on ZnO nanorods. Published version 2011-07-12T01:00:29Z 2019-12-06T18:45:14Z 2011-07-12T01:00:29Z 2019-12-06T18:45:14Z 2006 2006 Journal Article Wei, A., Sun, X., Wang, J. X., Lei, Y., Cai, X. P., Li, C. M., et al. (2006). Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition. Applied Physics Letters, 89. https://hdl.handle.net/10356/93770 http://hdl.handle.net/10220/6883 10.1063/1.2356307 en Applied physics letters © 2006 American Institute of Physics. This paper was published in Applied Physics Letters and is made available as an electronic reprint (preprint) with permission of American Institute of Physics. The paper can be found at: [DOI: http://dx.doi.org/10.1063/1.2356307]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 3 p. application/pdf |
| institution |
Nanyang Technological University |
| building |
NTU Library |
| continent |
Asia |
| country |
Singapore Singapore |
| content_provider |
NTU Library |
| collection |
DR-NTU |
| language |
English |
| topic |
DRNTU::Engineering::Materials::Biomaterials |
| spellingShingle |
DRNTU::Engineering::Materials::Biomaterials Lei, Y. Cai, X. P. Li, Chang Ming Dong, Zhili Huang, W. Wang, J. X. Wei, A. Sun, Xiaowei Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition |
| description |
We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction. At an applied potential of +0.8 V versus Ag/AgCl reference electrode, ZnO nanorods based biosensor presented a high and reproducible sensitivity of 23.1 μA cm−2 mM−1 with a response time of less than 5 s. The biosensor shows a linear range from 0.01 to 3.45 mM and an experiment limit of detection of 0.01 mM. An apparent Michaelis-Menten constant of 2.9 mM shows a high affinity between glucose and GOx immobilized on ZnO nanorods. |
| author2 |
School of Materials Science & Engineering |
| author_facet |
School of Materials Science & Engineering Lei, Y. Cai, X. P. Li, Chang Ming Dong, Zhili Huang, W. Wang, J. X. Wei, A. Sun, Xiaowei |
| format |
Article |
| author |
Lei, Y. Cai, X. P. Li, Chang Ming Dong, Zhili Huang, W. Wang, J. X. Wei, A. Sun, Xiaowei |
| author_sort |
Lei, Y. |
| title |
Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition |
| title_short |
Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition |
| title_full |
Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition |
| title_fullStr |
Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition |
| title_full_unstemmed |
Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition |
| title_sort |
enzymatic glucose biosensor based on zno nanorod array grown by hydrothermal decomposition |
| publishDate |
2011 |
| url |
https://hdl.handle.net/10356/93770 http://hdl.handle.net/10220/6883 |
| _version_ |
1772827027843317760 |