Molecular and structural characterization of the domain 2 of Hepatitis C virus non-structural protein 5A

Hepatitis C virus (HCV) non-structural protein 5A protein (NS5A), which consists of three functional domains, is involved in regulating viral replication, interferon resistance, and apoptosis. Recently, the three-dimensional structure of the domain 1 was determined. However, currently the molecular...

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Main Authors: Liang, Yu, Kang, Cong Bao, Yoon, Ho Sup
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2012
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Online Access:https://hdl.handle.net/10356/95125
http://hdl.handle.net/10220/8823
http://www.molcells.org/home/journal/
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-951252023-02-28T17:03:53Z Molecular and structural characterization of the domain 2 of Hepatitis C virus non-structural protein 5A Liang, Yu Kang, Cong Bao Yoon, Ho Sup School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology Hepatitis C virus (HCV) non-structural protein 5A protein (NS5A), which consists of three functional domains, is involved in regulating viral replication, interferon resistance, and apoptosis. Recently, the three-dimensional structure of the domain 1 was determined. However, currently the molecular basis for the domains 2 and 3 of HCV NS5A is yet to be defined. Toward this end, we expressed, purified the domain 2 of the NS5A (NS5A-D2), and then performed biochemical and structural studies. The purified domain 2 was active and was able to bind NS5B and PKR, biological partners of NS5A. The results from gel filtration, CD analysis, 1D 1H NMR and 2D 1H-15N heteronuclear single quantum correlation (HSQC) spectroscopy indicate that the domain 2 of NS5A appears to be flexible and disordered. Accepted version 2012-10-30T00:48:48Z 2019-12-06T19:08:43Z 2012-10-30T00:48:48Z 2019-12-06T19:08:43Z 2006 2006 Journal Article Liang, Y., Kang, C. B., & Yoon, H. S. (2006). Molecular and structural characterization of the domain 2 of Hepatitis C virus non-structural protein 5A. Molecules and Cells, 22(1), 13-20. https://hdl.handle.net/10356/95125 http://hdl.handle.net/10220/8823 http://www.molcells.org/home/journal/ en Molecules and cells © 2006 KSMCB. This is the author created version of a work that has been peer reviewed and accepted for publication by Molecules and Cells, KSMCB. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://www.molcells.org/home/journal/]. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology
spellingShingle DRNTU::Science::Biological sciences::Microbiology
Liang, Yu
Kang, Cong Bao
Yoon, Ho Sup
Molecular and structural characterization of the domain 2 of Hepatitis C virus non-structural protein 5A
description Hepatitis C virus (HCV) non-structural protein 5A protein (NS5A), which consists of three functional domains, is involved in regulating viral replication, interferon resistance, and apoptosis. Recently, the three-dimensional structure of the domain 1 was determined. However, currently the molecular basis for the domains 2 and 3 of HCV NS5A is yet to be defined. Toward this end, we expressed, purified the domain 2 of the NS5A (NS5A-D2), and then performed biochemical and structural studies. The purified domain 2 was active and was able to bind NS5B and PKR, biological partners of NS5A. The results from gel filtration, CD analysis, 1D 1H NMR and 2D 1H-15N heteronuclear single quantum correlation (HSQC) spectroscopy indicate that the domain 2 of NS5A appears to be flexible and disordered.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Liang, Yu
Kang, Cong Bao
Yoon, Ho Sup
format Article
author Liang, Yu
Kang, Cong Bao
Yoon, Ho Sup
author_sort Liang, Yu
title Molecular and structural characterization of the domain 2 of Hepatitis C virus non-structural protein 5A
title_short Molecular and structural characterization of the domain 2 of Hepatitis C virus non-structural protein 5A
title_full Molecular and structural characterization of the domain 2 of Hepatitis C virus non-structural protein 5A
title_fullStr Molecular and structural characterization of the domain 2 of Hepatitis C virus non-structural protein 5A
title_full_unstemmed Molecular and structural characterization of the domain 2 of Hepatitis C virus non-structural protein 5A
title_sort molecular and structural characterization of the domain 2 of hepatitis c virus non-structural protein 5a
publishDate 2012
url https://hdl.handle.net/10356/95125
http://hdl.handle.net/10220/8823
http://www.molcells.org/home/journal/
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