Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor

A nanoporous alumina membrane-based ultrasensitive DNA biosensor is constructed using 5′-aminated DNA probes immobilized onto the alumina channel walls. Alumina nanoporous membrane-like structure is carved over platinum wire electrode of 76 µm diameter dimension by electrochemical anodization. The h...

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Main Authors: Rai, Varun, Hapuarachchi, Hapuarachchige C., Ng, Lee Ching, Soh, Siew Hwa, Leo, Yee Sin, Toh, Chee-Seng
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/95452
http://hdl.handle.net/10220/9193
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-954522023-02-28T19:38:07Z Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor Rai, Varun Hapuarachchi, Hapuarachchige C. Ng, Lee Ching Soh, Siew Hwa Leo, Yee Sin Toh, Chee-Seng School of Physical and Mathematical Sciences A nanoporous alumina membrane-based ultrasensitive DNA biosensor is constructed using 5′-aminated DNA probes immobilized onto the alumina channel walls. Alumina nanoporous membrane-like structure is carved over platinum wire electrode of 76 µm diameter dimension by electrochemical anodization. The hybridization of complementary target DNA with probe DNA molecules attached inside the pores influences the pore size and ionic conductivity. The biosensor demonstrates linear range over 6 order of magnitude with ultrasensitive detection limit of 9.55×10−12 M for the quantification of ss-31 mer DNA sequence. Its applicability is challenged against real time cDNA PCR sample of dengue virus serotype1 derived from asymmetric PCR. Excellent specificity down to one nucleotide mismatch in target DNA sample of DENV3 is also demonstrated. Published version 2013-02-20T05:59:58Z 2019-12-06T19:15:11Z 2013-02-20T05:59:58Z 2019-12-06T19:15:11Z 2012 2012 Journal Article Rai, V., Hapuarachchi, H. C., Ng, L. C., Soh, S. H., Leo, Y. S., & Toh, C. S. (2012). Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor. PLoS ONE, 7(8). 1932-6203 https://hdl.handle.net/10356/95452 http://hdl.handle.net/10220/9193 10.1371/journal.pone.0042346 22927927 en PLoS ONE © 2012 The Authors. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
description A nanoporous alumina membrane-based ultrasensitive DNA biosensor is constructed using 5′-aminated DNA probes immobilized onto the alumina channel walls. Alumina nanoporous membrane-like structure is carved over platinum wire electrode of 76 µm diameter dimension by electrochemical anodization. The hybridization of complementary target DNA with probe DNA molecules attached inside the pores influences the pore size and ionic conductivity. The biosensor demonstrates linear range over 6 order of magnitude with ultrasensitive detection limit of 9.55×10−12 M for the quantification of ss-31 mer DNA sequence. Its applicability is challenged against real time cDNA PCR sample of dengue virus serotype1 derived from asymmetric PCR. Excellent specificity down to one nucleotide mismatch in target DNA sample of DENV3 is also demonstrated.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Rai, Varun
Hapuarachchi, Hapuarachchige C.
Ng, Lee Ching
Soh, Siew Hwa
Leo, Yee Sin
Toh, Chee-Seng
format Article
author Rai, Varun
Hapuarachchi, Hapuarachchige C.
Ng, Lee Ching
Soh, Siew Hwa
Leo, Yee Sin
Toh, Chee-Seng
spellingShingle Rai, Varun
Hapuarachchi, Hapuarachchige C.
Ng, Lee Ching
Soh, Siew Hwa
Leo, Yee Sin
Toh, Chee-Seng
Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor
author_sort Rai, Varun
title Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor
title_short Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor
title_full Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor
title_fullStr Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor
title_full_unstemmed Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor
title_sort ultrasensitive cdna detection of dengue virus rna using electrochemical nanoporous membrane-based biosensor
publishDate 2013
url https://hdl.handle.net/10356/95452
http://hdl.handle.net/10220/9193
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