Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp

An electrochemical nanoporous alumina membrane-based label free DNA biosensor is developed using 5′-aminated DNA probes immobilized into the nanochannels of alumina. Alumina nanoporous membrane-like structure is carved over platinum wire electrode of 76 μm diameter dimension by electrochemical anodi...

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Main Authors: Rai, Varun, Deng, Jiajia, Toh, Chee-Seng
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/95908
http://hdl.handle.net/10220/11880
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-959082020-03-07T12:37:22Z Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp Rai, Varun Deng, Jiajia Toh, Chee-Seng School of Physical and Mathematical Sciences An electrochemical nanoporous alumina membrane-based label free DNA biosensor is developed using 5′-aminated DNA probes immobilized into the nanochannels of alumina. Alumina nanoporous membrane-like structure is carved over platinum wire electrode of 76 μm diameter dimension by electrochemical anodization. The hybridization of complementary target DNA with probe DNA molecules attached inside the nanochannels influences the pore size and ionic conductivity. Electrochemical biosensing signal is derived from only redox species Fe(CN)64− across single wire Pt electrode. The biosensors sensing mechanism relies on the monitoring of electrode's Faradaic current response toward redox species, Fe(CN)64−, which is sensitive toward the hybridization of complementary target with probe DNA immobilized into the alumina nanochannels. The biosensor demonstrates wide linear range over 7 orders of magnitude with ultrasensitive detection limit 3.1×10−13 M for the quantification of ss 21mer DNA sequence and selectively differentiates the complementary sequence from target sequences with single base mismatch (MM1) and triple bases mismatch (MM3) of different strain of Legionella sp. Its applicability is also challenged against real time Legionella pneumophila genomic DNA sample derived from the asymmetric PCR method. 2013-07-18T06:41:43Z 2019-12-06T19:23:15Z 2013-07-18T06:41:43Z 2019-12-06T19:23:15Z 2012 2012 Journal Article Rai, V., Deng, J., & Toh, C.-S. (2012). Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp. Talanta, 98,112-117. 0039-9140 https://hdl.handle.net/10356/95908 http://hdl.handle.net/10220/11880 10.1016/j.talanta.2012.06.055 en Talanta © 2012 Elsevier B.V.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
description An electrochemical nanoporous alumina membrane-based label free DNA biosensor is developed using 5′-aminated DNA probes immobilized into the nanochannels of alumina. Alumina nanoporous membrane-like structure is carved over platinum wire electrode of 76 μm diameter dimension by electrochemical anodization. The hybridization of complementary target DNA with probe DNA molecules attached inside the nanochannels influences the pore size and ionic conductivity. Electrochemical biosensing signal is derived from only redox species Fe(CN)64− across single wire Pt electrode. The biosensors sensing mechanism relies on the monitoring of electrode's Faradaic current response toward redox species, Fe(CN)64−, which is sensitive toward the hybridization of complementary target with probe DNA immobilized into the alumina nanochannels. The biosensor demonstrates wide linear range over 7 orders of magnitude with ultrasensitive detection limit 3.1×10−13 M for the quantification of ss 21mer DNA sequence and selectively differentiates the complementary sequence from target sequences with single base mismatch (MM1) and triple bases mismatch (MM3) of different strain of Legionella sp. Its applicability is also challenged against real time Legionella pneumophila genomic DNA sample derived from the asymmetric PCR method.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Rai, Varun
Deng, Jiajia
Toh, Chee-Seng
format Article
author Rai, Varun
Deng, Jiajia
Toh, Chee-Seng
spellingShingle Rai, Varun
Deng, Jiajia
Toh, Chee-Seng
Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp
author_sort Rai, Varun
title Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp
title_short Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp
title_full Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp
title_fullStr Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp
title_full_unstemmed Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella sp
title_sort electrochemical nanoporous alumina membrane-based label-free dna biosensor for the detection of legionella sp
publishDate 2013
url https://hdl.handle.net/10356/95908
http://hdl.handle.net/10220/11880
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