Argon-plasma-induced ultrathin thermal grafting of thermoresponsive pNIPAm coating for contractile patterned human SMC sheet engineering

A new method for ultrathin grafting of pNIPAm on PDMS surfaces is introduced that employs plasma activation of the surface followed by thermal polymerization. This method is optimized for human primary SMC attachment and subsequent intact cell sheet detachment by lowering the temperature. The contra...

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Main Authors: Rayatpisheh, Shahrzad, Li, Peng, Chan-Park, Mary B.
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/96377
http://hdl.handle.net/10220/17320
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-963772020-03-07T11:35:36Z Argon-plasma-induced ultrathin thermal grafting of thermoresponsive pNIPAm coating for contractile patterned human SMC sheet engineering Rayatpisheh, Shahrzad Li, Peng Chan-Park, Mary B. School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering A new method for ultrathin grafting of pNIPAm on PDMS surfaces is introduced that employs plasma activation of the surface followed by thermal polymerization. This method is optimized for human primary SMC attachment and subsequent intact cell sheet detachment by lowering the temperature. The contractile gene expression of the cells showed that the contractile phenotype of the SMCs which is induced by aligning the cells through micropatterning is more preserved after thermoresponsive cell sheet detachment in contrast with enzymatic detachment. Given its simplicity and low cost, this thermoresponsive grafting method can be utilized for engineering patterned cell sheets for future bottom-up tissue engineering techniques. 2013-11-05T08:13:56Z 2019-12-06T19:29:39Z 2013-11-05T08:13:56Z 2019-12-06T19:29:39Z 2012 2012 Journal Article Rayatpisheh, S., Li, P., & Chan-Park, M. B. (2012). Argon-plasma-induced ultrathin thermal grafting of thermoresponsive pNIPAm coating for contractile patterned human SMC sheet engineering. Macromolecular bioscience, 12(7), 937-945. 1616-5195 https://hdl.handle.net/10356/96377 http://hdl.handle.net/10220/17320 10.1002/mabi.201100477 en Macromolecular bioscience
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Bioengineering
spellingShingle DRNTU::Engineering::Bioengineering
Rayatpisheh, Shahrzad
Li, Peng
Chan-Park, Mary B.
Argon-plasma-induced ultrathin thermal grafting of thermoresponsive pNIPAm coating for contractile patterned human SMC sheet engineering
description A new method for ultrathin grafting of pNIPAm on PDMS surfaces is introduced that employs plasma activation of the surface followed by thermal polymerization. This method is optimized for human primary SMC attachment and subsequent intact cell sheet detachment by lowering the temperature. The contractile gene expression of the cells showed that the contractile phenotype of the SMCs which is induced by aligning the cells through micropatterning is more preserved after thermoresponsive cell sheet detachment in contrast with enzymatic detachment. Given its simplicity and low cost, this thermoresponsive grafting method can be utilized for engineering patterned cell sheets for future bottom-up tissue engineering techniques.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Rayatpisheh, Shahrzad
Li, Peng
Chan-Park, Mary B.
format Article
author Rayatpisheh, Shahrzad
Li, Peng
Chan-Park, Mary B.
author_sort Rayatpisheh, Shahrzad
title Argon-plasma-induced ultrathin thermal grafting of thermoresponsive pNIPAm coating for contractile patterned human SMC sheet engineering
title_short Argon-plasma-induced ultrathin thermal grafting of thermoresponsive pNIPAm coating for contractile patterned human SMC sheet engineering
title_full Argon-plasma-induced ultrathin thermal grafting of thermoresponsive pNIPAm coating for contractile patterned human SMC sheet engineering
title_fullStr Argon-plasma-induced ultrathin thermal grafting of thermoresponsive pNIPAm coating for contractile patterned human SMC sheet engineering
title_full_unstemmed Argon-plasma-induced ultrathin thermal grafting of thermoresponsive pNIPAm coating for contractile patterned human SMC sheet engineering
title_sort argon-plasma-induced ultrathin thermal grafting of thermoresponsive pnipam coating for contractile patterned human smc sheet engineering
publishDate 2013
url https://hdl.handle.net/10356/96377
http://hdl.handle.net/10220/17320
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