Ultrasensitive colorimetric DNA detection using a combination of rolling circle amplification and Nicking Endonuclease-Assisted Nanoparticle Amplification (NEANA)

A combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA) is used for the rapid, colorimetric detection of DNA. The integration of rolling circle amplification into the NEANA approach allows for detection of oligonucleotides with arbitrary seq...

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Bibliographic Details
Main Authors: Xu, Wei, Xie, Xiaoji, Li, Dawei, Yang, Zhaoqi, Li, Tianhu, Liu, Xiaogang
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/96612
http://hdl.handle.net/10220/10361
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Institution: Nanyang Technological University
Language: English
Description
Summary:A combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA) is used for the rapid, colorimetric detection of DNA. The integration of rolling circle amplification into the NEANA approach allows for detection of oligonucleotides with arbitrary sequences at ultralow concentrations.