Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane

We previously reported that mitotic endoplasmic reticulum (ER) membrane cisternae or sheets directly assemble mammalian nuclear envelope (NE) at the end of mitosis. In this study, we investigated the dynamics of the high curvature regions of partially assembled nuclear envelope membrane using reti...

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Main Authors: Lu, Lei, Kirchhausen, Tomas.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/96720
http://hdl.handle.net/10220/13053
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291306/
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-967202023-02-28T16:59:13Z Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane Lu, Lei Kirchhausen, Tomas. School of Biological Sciences DRNTU::Science::Biological sciences We previously reported that mitotic endoplasmic reticulum (ER) membrane cisternae or sheets directly assemble mammalian nuclear envelope (NE) at the end of mitosis. In this study, we investigated the dynamics of the high curvature regions of partially assembled nuclear envelope membrane using reticulon4a as a probe. We found that, after sorting out reticulon4a from the nascent NE membrane sheets, reticulon4a is specifically localized to the leading edges. Our 3D time lapse images suggested that ER tubules could be incompetent in assembling the NE membrane. Our findings suggest a possible role of reticulons at the leading edges during the NE re-assembly and provide further evidences that the mitotic assembly of NE is by ER cisternae rather than tubules. Accepted version 2013-08-06T04:45:51Z 2019-12-06T19:34:15Z 2013-08-06T04:45:51Z 2019-12-06T19:34:15Z 2011 2011 Journal Article https://hdl.handle.net/10356/96720 http://hdl.handle.net/10220/13053 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291306/ en Communicative & integrative biology © 2012 Landes Bioscience. This is the author created version of a work that has been peer reviewed and accepted for publication by Communicative & integrative biology, Landes Bioscience. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291306/]. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Lu, Lei
Kirchhausen, Tomas.
Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane
description We previously reported that mitotic endoplasmic reticulum (ER) membrane cisternae or sheets directly assemble mammalian nuclear envelope (NE) at the end of mitosis. In this study, we investigated the dynamics of the high curvature regions of partially assembled nuclear envelope membrane using reticulon4a as a probe. We found that, after sorting out reticulon4a from the nascent NE membrane sheets, reticulon4a is specifically localized to the leading edges. Our 3D time lapse images suggested that ER tubules could be incompetent in assembling the NE membrane. Our findings suggest a possible role of reticulons at the leading edges during the NE re-assembly and provide further evidences that the mitotic assembly of NE is by ER cisternae rather than tubules.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Lu, Lei
Kirchhausen, Tomas.
format Article
author Lu, Lei
Kirchhausen, Tomas.
author_sort Lu, Lei
title Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane
title_short Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane
title_full Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane
title_fullStr Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane
title_full_unstemmed Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane
title_sort visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane
publishDate 2013
url https://hdl.handle.net/10356/96720
http://hdl.handle.net/10220/13053
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291306/
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