Surface functionalization of nanoparticles to control cell interactions and drug release

Nanoparticles made from poly(dl-lactide-co-glycolide) (PLGA) are used to deliver a wide range of bioactive molecules, due to their biocompatibility and biodegradability. This study investigates the surface modification of PLGA nanoparticles via the layer-by-layer (LbL) deposition of polyelectrolytes...

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Main Authors: Luo, Rongcong, Neu, Björn, Venkatraman, Subbu S.
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/96740
http://hdl.handle.net/10220/10390
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-967402020-06-01T10:26:33Z Surface functionalization of nanoparticles to control cell interactions and drug release Luo, Rongcong Neu, Björn Venkatraman, Subbu S. School of Chemical and Biomedical Engineering School of Materials Science & Engineering Nanoparticles made from poly(dl-lactide-co-glycolide) (PLGA) are used to deliver a wide range of bioactive molecules, due to their biocompatibility and biodegradability. This study investigates the surface modification of PLGA nanoparticles via the layer-by-layer (LbL) deposition of polyelectrolytes, and the effects of these coatings on the release behavior, cytotoxicity, hemolytic activity, and cellular uptake efficiency. PLGA nanoparticles are modified via LbL adsorption of two polyelectrolyte pairs: 1) poly(allylamine hydrochloride) (PAH) and poly(styrene sulfonate) (PSS) and 2) poly(L-lysine hydrobromide) (PLL) and dextran sulfate (DES). It is demonstrated that both PAH/PSS and PLL/DES coatings suppress the burst release usually observed for unmodified PLGA nanoparticles and that the release behavior can be adjusted by changing the layer numbers, layer materials, or by crosslinking the layer constituents. Neither bare nor polyelectrolyte-modified PLGA nanoparticles show any signs of cytotoxicity. However, nanoparticles with a positively charged polyelectrolyte as the outermost layer induce hemolysis, whereas uncoated particles or particles with a negatively charged polyelectrolyte as the outermost layer show no hemolytic activity. Furthermore, particles with either PAH or PLL as the outermost layer also demonstrate a higher uptake efficiency by L929 fibroblast cells, due to a higher cell–particle affinity. This study suggests that LbL coating of PLGA nanoparticles can control the release behavior of bioactive molecules as well as the surface activity, therefore providing a promising strategy to enhance the efficiency of nanoparticulate drug-delivery systems. 2013-06-14T02:56:21Z 2019-12-06T19:34:26Z 2013-06-14T02:56:21Z 2019-12-06T19:34:26Z 2012 2012 Journal Article Luo, R., Neu, B., & Venkatraman, S. S. (2012). Surface Functionalization of Nanoparticles to Control Cell Interactions and Drug Release. Small, 8(16), 2585-2594. 1613-6810 https://hdl.handle.net/10356/96740 http://hdl.handle.net/10220/10390 10.1002/smll.201200398 en Small © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
institution Nanyang Technological University
building NTU Library
country Singapore
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language English
description Nanoparticles made from poly(dl-lactide-co-glycolide) (PLGA) are used to deliver a wide range of bioactive molecules, due to their biocompatibility and biodegradability. This study investigates the surface modification of PLGA nanoparticles via the layer-by-layer (LbL) deposition of polyelectrolytes, and the effects of these coatings on the release behavior, cytotoxicity, hemolytic activity, and cellular uptake efficiency. PLGA nanoparticles are modified via LbL adsorption of two polyelectrolyte pairs: 1) poly(allylamine hydrochloride) (PAH) and poly(styrene sulfonate) (PSS) and 2) poly(L-lysine hydrobromide) (PLL) and dextran sulfate (DES). It is demonstrated that both PAH/PSS and PLL/DES coatings suppress the burst release usually observed for unmodified PLGA nanoparticles and that the release behavior can be adjusted by changing the layer numbers, layer materials, or by crosslinking the layer constituents. Neither bare nor polyelectrolyte-modified PLGA nanoparticles show any signs of cytotoxicity. However, nanoparticles with a positively charged polyelectrolyte as the outermost layer induce hemolysis, whereas uncoated particles or particles with a negatively charged polyelectrolyte as the outermost layer show no hemolytic activity. Furthermore, particles with either PAH or PLL as the outermost layer also demonstrate a higher uptake efficiency by L929 fibroblast cells, due to a higher cell–particle affinity. This study suggests that LbL coating of PLGA nanoparticles can control the release behavior of bioactive molecules as well as the surface activity, therefore providing a promising strategy to enhance the efficiency of nanoparticulate drug-delivery systems.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Luo, Rongcong
Neu, Björn
Venkatraman, Subbu S.
format Article
author Luo, Rongcong
Neu, Björn
Venkatraman, Subbu S.
spellingShingle Luo, Rongcong
Neu, Björn
Venkatraman, Subbu S.
Surface functionalization of nanoparticles to control cell interactions and drug release
author_sort Luo, Rongcong
title Surface functionalization of nanoparticles to control cell interactions and drug release
title_short Surface functionalization of nanoparticles to control cell interactions and drug release
title_full Surface functionalization of nanoparticles to control cell interactions and drug release
title_fullStr Surface functionalization of nanoparticles to control cell interactions and drug release
title_full_unstemmed Surface functionalization of nanoparticles to control cell interactions and drug release
title_sort surface functionalization of nanoparticles to control cell interactions and drug release
publishDate 2013
url https://hdl.handle.net/10356/96740
http://hdl.handle.net/10220/10390
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