A quantitative proteomics approach in the study of microRNA 181a in HepG2 cells

Hepatocellular carcinoma (HCC) is currently of great concern due to its poor patient outcome despite the various therapies available. The cause of HCC usually stems from liver cirrhosis, which mainly results from Hepatitis B virus (HBV) or Hepatitis C virus (HCV) infection or environmental and genet...

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Main Authors: Habib, Nagy A., Tan, Jane Yi Lin, Chen, William Wei Ning
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/96804
http://hdl.handle.net/10220/13199
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-968042020-03-07T11:35:28Z A quantitative proteomics approach in the study of microRNA 181a in HepG2 cells Habib, Nagy A. Tan, Jane Yi Lin Chen, William Wei Ning School of Chemical and Biomedical Engineering Hepatocellular carcinoma (HCC) is currently of great concern due to its poor patient outcome despite the various therapies available. The cause of HCC usually stems from liver cirrhosis, which mainly results from Hepatitis B virus (HBV) or Hepatitis C virus (HCV) infection or environmental and genetic risk factors like alcoholism. Despite the preventive measures available now, the incidence and mortality rates are still high due to chronic infection, making it the third leading cause of cancer -related deaths in the world today and claiming approximately 600,000 lives annually. Patients are usually also diagnosed with HCC later in the stages, leading to a very poor prognosis. It is therefore imperative that a more effective treatment method is developed such that patient survival rates may be improved from current statistics of less than 50%. The role of microRNAs (miRNAs) in the regulation of gene expression and cellular development makes it an important player in cancer initiation and progression. Mir-181a has been shown to be an important miRNA involved in HCC. In this study, we propose to investigate the potential effects of miR-181a in HepG2 cells and also to explore the mechanisms in which it works in controlling cell fate. As chemotherapy is widely used in liver cancer treatment, we also study the use of miR-181a along with chemotherapy (i.e. Cisplatin). Using iTRAQ-coupled 2D LC-MS/MS analysis, we report here the study of protein profile of HepG2 cells transfected with miR-181a mimic and inhibitor separately. Three main types of cellular proteins including metabolic enzymes, protein binding and stress proteins displayed changes. The changes in the level of proteins involved in important cancer processes like cell growth were further supported by a western blot analysis. MiR-181a was subsequently found to increase HepG2 cell viability while the inhibitor displayed the opposite effect. The inhibitor also sensitized HepG2 cells to cisplatin. Cell cycle analysis showed that the inhibitor retards cell cycle progression by decreasing the proportion of cells in S and G2/M phases. Our findings therefore provide molecular evidence on the mechanism of action of miR-181a inhibitor, including its beneficial effects in inhibiting the development of HCC. 2013-08-23T03:37:43Z 2019-12-06T19:35:17Z 2013-08-23T03:37:43Z 2019-12-06T19:35:17Z 2012 2012 Journal Article Lin Jane Tan, Y., A. Habib, N., & Chen, W. N. W. (2012). A quantitative proteomics approach in the study of microRNA 181a in HepG2 cells. Current proteomics, 9(4), 262-271. https://hdl.handle.net/10356/96804 http://hdl.handle.net/10220/13199 10.2174/157016412805219170 en Current proteomics
institution Nanyang Technological University
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description Hepatocellular carcinoma (HCC) is currently of great concern due to its poor patient outcome despite the various therapies available. The cause of HCC usually stems from liver cirrhosis, which mainly results from Hepatitis B virus (HBV) or Hepatitis C virus (HCV) infection or environmental and genetic risk factors like alcoholism. Despite the preventive measures available now, the incidence and mortality rates are still high due to chronic infection, making it the third leading cause of cancer -related deaths in the world today and claiming approximately 600,000 lives annually. Patients are usually also diagnosed with HCC later in the stages, leading to a very poor prognosis. It is therefore imperative that a more effective treatment method is developed such that patient survival rates may be improved from current statistics of less than 50%. The role of microRNAs (miRNAs) in the regulation of gene expression and cellular development makes it an important player in cancer initiation and progression. Mir-181a has been shown to be an important miRNA involved in HCC. In this study, we propose to investigate the potential effects of miR-181a in HepG2 cells and also to explore the mechanisms in which it works in controlling cell fate. As chemotherapy is widely used in liver cancer treatment, we also study the use of miR-181a along with chemotherapy (i.e. Cisplatin). Using iTRAQ-coupled 2D LC-MS/MS analysis, we report here the study of protein profile of HepG2 cells transfected with miR-181a mimic and inhibitor separately. Three main types of cellular proteins including metabolic enzymes, protein binding and stress proteins displayed changes. The changes in the level of proteins involved in important cancer processes like cell growth were further supported by a western blot analysis. MiR-181a was subsequently found to increase HepG2 cell viability while the inhibitor displayed the opposite effect. The inhibitor also sensitized HepG2 cells to cisplatin. Cell cycle analysis showed that the inhibitor retards cell cycle progression by decreasing the proportion of cells in S and G2/M phases. Our findings therefore provide molecular evidence on the mechanism of action of miR-181a inhibitor, including its beneficial effects in inhibiting the development of HCC.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Habib, Nagy A.
Tan, Jane Yi Lin
Chen, William Wei Ning
format Article
author Habib, Nagy A.
Tan, Jane Yi Lin
Chen, William Wei Ning
spellingShingle Habib, Nagy A.
Tan, Jane Yi Lin
Chen, William Wei Ning
A quantitative proteomics approach in the study of microRNA 181a in HepG2 cells
author_sort Habib, Nagy A.
title A quantitative proteomics approach in the study of microRNA 181a in HepG2 cells
title_short A quantitative proteomics approach in the study of microRNA 181a in HepG2 cells
title_full A quantitative proteomics approach in the study of microRNA 181a in HepG2 cells
title_fullStr A quantitative proteomics approach in the study of microRNA 181a in HepG2 cells
title_full_unstemmed A quantitative proteomics approach in the study of microRNA 181a in HepG2 cells
title_sort quantitative proteomics approach in the study of microrna 181a in hepg2 cells
publishDate 2013
url https://hdl.handle.net/10356/96804
http://hdl.handle.net/10220/13199
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