Electrospinning pure protein solutions in core-shell fibers

Electrospinning of protein-loaded fibers faces many challenges, e.g. burst release owing to segregation of the protein on the fiber surface, loss of activity due to electrospinning conditions, limitation of loading capacity etc. Core–shell electrospinning provides an effective way to electrospin fib...

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Main Authors: Tiwari, Sandeep Kumar, Venkatraman, Subbu S.
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/97160
http://hdl.handle.net/10220/10547
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-971602020-06-01T10:01:57Z Electrospinning pure protein solutions in core-shell fibers Tiwari, Sandeep Kumar Venkatraman, Subbu S. School of Materials Science & Engineering Electrospinning of protein-loaded fibers faces many challenges, e.g. burst release owing to segregation of the protein on the fiber surface, loss of activity due to electrospinning conditions, limitation of loading capacity etc. Core–shell electrospinning provides an effective way to electrospin fibers wherein the core can be loaded with bioactive molecules in friendly conditions of a compatible polymer solution, thereby protecting the molecules from the electrostatic field and organic solvent of shell solutions. The shell polymer, after the electrospinning, acts as a barrier to control the release of the loaded molecules. However, the limitation of loading capacity still remains due the prerequisite of using an additional polymer as additive to achieve the minimum viscosity of the core solution required for viscous drag by the shell solution being drawn by the electrostatic force. The work reported here aims to alleviate the need of a polymer additive by using aqueous protein solutions of very high concentration. High concentrations of protein solutions were successfully electrospun as the core of the protein–poly(lactide-co-glycolic acid) core–shell fibers. A partitioning effect was seen in the controlled release of hydrophilic proteins as they were retained in the aqueous core for longer times. Using lysozyme as a model protein, it was shown that the activity is significantly retained after electrospinning, compared with electrospinning in monolithic fibers. Moreover, the lysozyme activity was also comparable with the lysozyme released from core–shell fibers spun using poly(vinyl acetate) as additive in the core. 2013-06-24T07:52:10Z 2019-12-06T19:39:32Z 2013-06-24T07:52:10Z 2019-12-06T19:39:32Z 2012 2012 Journal Article Tiwari, S. K., & Venkatraman, S. (2012). Electrospinning pure protein solutions in core-shell fibers. Polymer International, 61(10), 1549-1555. 0959-8103 https://hdl.handle.net/10356/97160 http://hdl.handle.net/10220/10547 10.1002/pi.4246 en Polymer International © 2012 Society of Chemical Industry.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
description Electrospinning of protein-loaded fibers faces many challenges, e.g. burst release owing to segregation of the protein on the fiber surface, loss of activity due to electrospinning conditions, limitation of loading capacity etc. Core–shell electrospinning provides an effective way to electrospin fibers wherein the core can be loaded with bioactive molecules in friendly conditions of a compatible polymer solution, thereby protecting the molecules from the electrostatic field and organic solvent of shell solutions. The shell polymer, after the electrospinning, acts as a barrier to control the release of the loaded molecules. However, the limitation of loading capacity still remains due the prerequisite of using an additional polymer as additive to achieve the minimum viscosity of the core solution required for viscous drag by the shell solution being drawn by the electrostatic force. The work reported here aims to alleviate the need of a polymer additive by using aqueous protein solutions of very high concentration. High concentrations of protein solutions were successfully electrospun as the core of the protein–poly(lactide-co-glycolic acid) core–shell fibers. A partitioning effect was seen in the controlled release of hydrophilic proteins as they were retained in the aqueous core for longer times. Using lysozyme as a model protein, it was shown that the activity is significantly retained after electrospinning, compared with electrospinning in monolithic fibers. Moreover, the lysozyme activity was also comparable with the lysozyme released from core–shell fibers spun using poly(vinyl acetate) as additive in the core.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Tiwari, Sandeep Kumar
Venkatraman, Subbu S.
format Article
author Tiwari, Sandeep Kumar
Venkatraman, Subbu S.
spellingShingle Tiwari, Sandeep Kumar
Venkatraman, Subbu S.
Electrospinning pure protein solutions in core-shell fibers
author_sort Tiwari, Sandeep Kumar
title Electrospinning pure protein solutions in core-shell fibers
title_short Electrospinning pure protein solutions in core-shell fibers
title_full Electrospinning pure protein solutions in core-shell fibers
title_fullStr Electrospinning pure protein solutions in core-shell fibers
title_full_unstemmed Electrospinning pure protein solutions in core-shell fibers
title_sort electrospinning pure protein solutions in core-shell fibers
publishDate 2013
url https://hdl.handle.net/10356/97160
http://hdl.handle.net/10220/10547
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