Continuous cell separation using dielectrophoresis through asymmetric and periodic microelectrode array

The study presents a dielectrophoretic cell separation method via three-dimensional (3D) nonuniform electric fields generated by employing a periodic array of discrete but locally asymmetric triangular bottom microelectrodes and a continuous top electrode. Traversing through the microelectrodes, het...

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Main Authors: Chian, Kerm Sin (MAE), Ling, Siang Hooi, Lam, Yee Cheong
Other Authors: School of Mechanical and Aerospace Engineering
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/97203
http://hdl.handle.net/10220/10756
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-972032020-03-07T13:22:17Z Continuous cell separation using dielectrophoresis through asymmetric and periodic microelectrode array Chian, Kerm Sin (MAE) Ling, Siang Hooi Lam, Yee Cheong School of Mechanical and Aerospace Engineering The study presents a dielectrophoretic cell separation method via three-dimensional (3D) nonuniform electric fields generated by employing a periodic array of discrete but locally asymmetric triangular bottom microelectrodes and a continuous top electrode. Traversing through the microelectrodes, heterogeneous cells are electrically polarized to experience different strengths of positive dielectrophoretic forces, in response to the 3D nonuniform electric fields. The cells that experience stronger positive dielectrophoresis are streamed further in the perpendicular direction to the fluid flow, leaving the cells that experience weak positive dielectrophoresis, which continue to traverse the microelectrode array essentially along the laminar flow streamlines. The proposed method has achieved 87.3% pure live cells harvesting efficiency from a live/dead NIH-3T3 cells mixture, and separation of MG-63 cells from erythrocytes with a separation efficiency of 82.8%. The demonstrated cell separation shows promising applications of the DEP separator for cell separation in a continuous mode. 2013-06-27T02:04:26Z 2019-12-06T19:40:10Z 2013-06-27T02:04:26Z 2019-12-06T19:40:10Z 2012 2012 Journal Article Ling, S. H., Lam, Y. C., & Chian, K. S. (2012). Continuous Cell Separation Using Dielectrophoresis through Asymmetric and Periodic Microelectrode Array. Analytical Chemistry, 84(15), 6463-6470. 0003-2700 https://hdl.handle.net/10356/97203 http://hdl.handle.net/10220/10756 10.1021/ac300079q en Analytical chemistry © 2012 American Chemical Society.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
description The study presents a dielectrophoretic cell separation method via three-dimensional (3D) nonuniform electric fields generated by employing a periodic array of discrete but locally asymmetric triangular bottom microelectrodes and a continuous top electrode. Traversing through the microelectrodes, heterogeneous cells are electrically polarized to experience different strengths of positive dielectrophoretic forces, in response to the 3D nonuniform electric fields. The cells that experience stronger positive dielectrophoresis are streamed further in the perpendicular direction to the fluid flow, leaving the cells that experience weak positive dielectrophoresis, which continue to traverse the microelectrode array essentially along the laminar flow streamlines. The proposed method has achieved 87.3% pure live cells harvesting efficiency from a live/dead NIH-3T3 cells mixture, and separation of MG-63 cells from erythrocytes with a separation efficiency of 82.8%. The demonstrated cell separation shows promising applications of the DEP separator for cell separation in a continuous mode.
author2 School of Mechanical and Aerospace Engineering
author_facet School of Mechanical and Aerospace Engineering
Chian, Kerm Sin (MAE)
Ling, Siang Hooi
Lam, Yee Cheong
format Article
author Chian, Kerm Sin (MAE)
Ling, Siang Hooi
Lam, Yee Cheong
spellingShingle Chian, Kerm Sin (MAE)
Ling, Siang Hooi
Lam, Yee Cheong
Continuous cell separation using dielectrophoresis through asymmetric and periodic microelectrode array
author_sort Chian, Kerm Sin (MAE)
title Continuous cell separation using dielectrophoresis through asymmetric and periodic microelectrode array
title_short Continuous cell separation using dielectrophoresis through asymmetric and periodic microelectrode array
title_full Continuous cell separation using dielectrophoresis through asymmetric and periodic microelectrode array
title_fullStr Continuous cell separation using dielectrophoresis through asymmetric and periodic microelectrode array
title_full_unstemmed Continuous cell separation using dielectrophoresis through asymmetric and periodic microelectrode array
title_sort continuous cell separation using dielectrophoresis through asymmetric and periodic microelectrode array
publishDate 2013
url https://hdl.handle.net/10356/97203
http://hdl.handle.net/10220/10756
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