Bacterial pathogen surface plasmon resonance biosensor advanced by long range surface plasmons and magnetic nanoparticle assays
A new approach to surface plasmon resonance (SPR) biosensors for rapid and highly sensitive detection of bacterial pathogens is reported. It is based on the spectroscopy of grating-coupled long-range surface plasmons (LRSPs) combined with magnetic nanoparticle (MNP) assay. The interrogation of LRSPs...
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sg-ntu-dr.10356-972072020-06-01T10:01:35Z Bacterial pathogen surface plasmon resonance biosensor advanced by long range surface plasmons and magnetic nanoparticle assays Wang, Yi Knoll, Wolfgang Dostálek, Jakub School of Materials Science & Engineering A new approach to surface plasmon resonance (SPR) biosensors for rapid and highly sensitive detection of bacterial pathogens is reported. It is based on the spectroscopy of grating-coupled long-range surface plasmons (LRSPs) combined with magnetic nanoparticle (MNP) assay. The interrogation of LRSPs allows increasing the biosensor figure of merit (FOM), and the employment of MNPs further enhances the sensor response by a fast delivery of the analyte to the sensor surface and through the amplified refractive index changes associated with the capture of target analyte. This amplification strategy is particularly attractive for detection of large analytes that diffuse slowly from the analyzed sample to the sensor surface. The potential of the presented approach is demonstrated in a model experiment in which Escherichia coli O157:H7 was detected at concentrations as low as 50 cfu mL–1, 4 orders of magnitude better than the limit of detection achieved by regular grating-coupled SPR with direct detection format. 2013-06-27T02:01:20Z 2019-12-06T19:40:12Z 2013-06-27T02:01:20Z 2019-12-06T19:40:12Z 2012 2012 Journal Article Wang, Y., Knoll, W., & Dostalek, J. (2012). Bacterial pathogen surface plasmon resonance biosensor advanced by long range surface plasmons and magnetic nanoparticle assays. Analytical chemistry, 84(19), 8345-8350. 0003-2700 https://hdl.handle.net/10356/97207 http://hdl.handle.net/10220/10755 10.1021/ac301904x en Analytical chemistry © 2012 American Chemical Society. |
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A new approach to surface plasmon resonance (SPR) biosensors for rapid and highly sensitive detection of bacterial pathogens is reported. It is based on the spectroscopy of grating-coupled long-range surface plasmons (LRSPs) combined with magnetic nanoparticle (MNP) assay. The interrogation of LRSPs allows increasing the biosensor figure of merit (FOM), and the employment of MNPs further enhances the sensor response by a fast delivery of the analyte to the sensor surface and through the amplified refractive index changes associated with the capture of target analyte. This amplification strategy is particularly attractive for detection of large analytes that diffuse slowly from the analyzed sample to the sensor surface. The potential of the presented approach is demonstrated in a model experiment in which Escherichia coli O157:H7 was detected at concentrations as low as 50 cfu mL–1, 4 orders of magnitude better than the limit of detection achieved by regular grating-coupled SPR with direct detection format. |
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School of Materials Science & Engineering |
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School of Materials Science & Engineering Wang, Yi Knoll, Wolfgang Dostálek, Jakub |
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Wang, Yi Knoll, Wolfgang Dostálek, Jakub |
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Wang, Yi Knoll, Wolfgang Dostálek, Jakub Bacterial pathogen surface plasmon resonance biosensor advanced by long range surface plasmons and magnetic nanoparticle assays |
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Wang, Yi |
title |
Bacterial pathogen surface plasmon resonance biosensor advanced by long range surface plasmons and magnetic nanoparticle assays |
title_short |
Bacterial pathogen surface plasmon resonance biosensor advanced by long range surface plasmons and magnetic nanoparticle assays |
title_full |
Bacterial pathogen surface plasmon resonance biosensor advanced by long range surface plasmons and magnetic nanoparticle assays |
title_fullStr |
Bacterial pathogen surface plasmon resonance biosensor advanced by long range surface plasmons and magnetic nanoparticle assays |
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Bacterial pathogen surface plasmon resonance biosensor advanced by long range surface plasmons and magnetic nanoparticle assays |
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bacterial pathogen surface plasmon resonance biosensor advanced by long range surface plasmons and magnetic nanoparticle assays |
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2013 |
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https://hdl.handle.net/10356/97207 http://hdl.handle.net/10220/10755 |
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