Quantum-dot-based biosensor for simultaneous detection of biomarker and therapeutic drug : first steps toward an assay for quantitative pharmacology

Quantitative pharmacology (QP) is often used to study the recommended dose of a drug. Using colorectal cancer as a model, the relationship between exposure (cetuximab, C225, therapeutic drug of colorectal cancer) and the biomarker response (carcinoembryonic antigen, CEA, biomarker of colorectal canc...

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Main Authors: Yang, Chi, Xu, Chunxiang, Wang, Xuemei, Hu, Xiao
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/97244
http://hdl.handle.net/10220/10750
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-972442020-06-01T10:21:22Z Quantum-dot-based biosensor for simultaneous detection of biomarker and therapeutic drug : first steps toward an assay for quantitative pharmacology Yang, Chi Xu, Chunxiang Wang, Xuemei Hu, Xiao School of Materials Science & Engineering Quantitative pharmacology (QP) is often used to study the recommended dose of a drug. Using colorectal cancer as a model, the relationship between exposure (cetuximab, C225, therapeutic drug of colorectal cancer) and the biomarker response (carcinoembryonic antigen, CEA, biomarker of colorectal cancer) is the core for the QP of C225. Thus, simultaneous detection of CEA and C225 is the first step towards an assay for the QP of C225. We developed a quantum dot (QD)-based multi-analyte biosensor for the simultaneous determination of CEA and the corresponding therapeutic drug C225. The assay is based on the competition between a quantum-dots-labeled glycans and target glycans using lectins as the recognition element. The dual-analytic biosensor detected CEA and C225 in the range from 1 ng mL−1 to 400 μg mL−1 by stripping voltammetry. The biosensor developed here for the simultaneous detection of the biomarkers and therapeutic drugs is expected to develop as a potential technique for quantitative pharmacology. 2013-06-27T01:41:11Z 2019-12-06T19:40:33Z 2013-06-27T01:41:11Z 2019-12-06T19:40:33Z 2012 2012 Journal Article Yang, C., Xu, C., Wang, X., & Hu, X. (2012). Quantum-dot-based biosensor for simultaneous detection of biomarker and therapeutic drug: first steps toward an assay for quantitative pharmacology. Analyst, 137(5), 1205-1209. 0003-2654 https://hdl.handle.net/10356/97244 http://hdl.handle.net/10220/10750 10.1039/c2an15894a en Analyst © 2012 The Royal Society of Chemistry.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
description Quantitative pharmacology (QP) is often used to study the recommended dose of a drug. Using colorectal cancer as a model, the relationship between exposure (cetuximab, C225, therapeutic drug of colorectal cancer) and the biomarker response (carcinoembryonic antigen, CEA, biomarker of colorectal cancer) is the core for the QP of C225. Thus, simultaneous detection of CEA and C225 is the first step towards an assay for the QP of C225. We developed a quantum dot (QD)-based multi-analyte biosensor for the simultaneous determination of CEA and the corresponding therapeutic drug C225. The assay is based on the competition between a quantum-dots-labeled glycans and target glycans using lectins as the recognition element. The dual-analytic biosensor detected CEA and C225 in the range from 1 ng mL−1 to 400 μg mL−1 by stripping voltammetry. The biosensor developed here for the simultaneous detection of the biomarkers and therapeutic drugs is expected to develop as a potential technique for quantitative pharmacology.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Yang, Chi
Xu, Chunxiang
Wang, Xuemei
Hu, Xiao
format Article
author Yang, Chi
Xu, Chunxiang
Wang, Xuemei
Hu, Xiao
spellingShingle Yang, Chi
Xu, Chunxiang
Wang, Xuemei
Hu, Xiao
Quantum-dot-based biosensor for simultaneous detection of biomarker and therapeutic drug : first steps toward an assay for quantitative pharmacology
author_sort Yang, Chi
title Quantum-dot-based biosensor for simultaneous detection of biomarker and therapeutic drug : first steps toward an assay for quantitative pharmacology
title_short Quantum-dot-based biosensor for simultaneous detection of biomarker and therapeutic drug : first steps toward an assay for quantitative pharmacology
title_full Quantum-dot-based biosensor for simultaneous detection of biomarker and therapeutic drug : first steps toward an assay for quantitative pharmacology
title_fullStr Quantum-dot-based biosensor for simultaneous detection of biomarker and therapeutic drug : first steps toward an assay for quantitative pharmacology
title_full_unstemmed Quantum-dot-based biosensor for simultaneous detection of biomarker and therapeutic drug : first steps toward an assay for quantitative pharmacology
title_sort quantum-dot-based biosensor for simultaneous detection of biomarker and therapeutic drug : first steps toward an assay for quantitative pharmacology
publishDate 2013
url https://hdl.handle.net/10356/97244
http://hdl.handle.net/10220/10750
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