Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure
A unique delivery system to reversibly and controllably load and release proteins under physiological conditions is desirable for protein therapeutics. We fabricate an ultrafast exponentially growing nanoporous multilayer structure comprised of two weak polyelectrolytes, poly(ethyleneimine) and algi...
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sg-ntu-dr.10356-972522020-03-07T11:40:21Z Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure Yuan, Weiyong Lu, Zhisong Wang, Huili Li, Chang Ming School of Chemical and Biomedical Engineering School of Mechanical and Aerospace Engineering Centre for Advanced Bionanosystems A unique delivery system to reversibly and controllably load and release proteins under physiological conditions is desirable for protein therapeutics. We fabricate an ultrafast exponentially growing nanoporous multilayer structure comprised of two weak polyelectrolytes, poly(ethyleneimine) and alginate with thickness and chemical composition controlled by the assembly pH. For the first time, the assembled multilayered structure demonstrates stimuli-free reversible protein loading and release capability at physiological conditions by a synthetic material. The protein loading and release time can also be controlled by the assembled bilayer number. The highest loading capacity for the target protein and longest release time of proteins for layer-by-layer films reported to date have been achieved with a 15-bilayered film fabricated in this work. The prominent properties of the assembled film provide great potential for various biomedical applications, especially as a delivery system for protein therapeutics. 2013-06-17T04:07:51Z 2019-12-06T19:40:36Z 2013-06-17T04:07:51Z 2019-12-06T19:40:36Z 2012 2012 Journal Article Yuan, W., Lu, Z., Wang, H., & Li, C. M. (2012). Stimuli-Free Reversible and Controllable Loading and Release of Proteins under Physiological Conditions by Exponentially Growing Nanoporous Multilayered Structure. Advanced Functional Materials, 22(9), 1932-1939. 1616-3028 https://hdl.handle.net/10356/97252 http://hdl.handle.net/10220/10429 10.1002/adfm.201102308 en Advanced functional materials © 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
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A unique delivery system to reversibly and controllably load and release proteins under physiological conditions is desirable for protein therapeutics. We fabricate an ultrafast exponentially growing nanoporous multilayer structure comprised of two weak polyelectrolytes, poly(ethyleneimine) and alginate with thickness and chemical composition controlled by the assembly pH. For the first time, the assembled multilayered structure demonstrates stimuli-free reversible protein loading and release capability at physiological conditions by a synthetic material. The protein loading and release time can also be controlled by the assembled bilayer number. The highest loading capacity for the target protein and longest release time of proteins for layer-by-layer films reported to date have been achieved with a 15-bilayered film fabricated in this work. The prominent properties of the assembled film provide great potential for various biomedical applications, especially as a delivery system for protein therapeutics. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Yuan, Weiyong Lu, Zhisong Wang, Huili Li, Chang Ming |
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Yuan, Weiyong Lu, Zhisong Wang, Huili Li, Chang Ming |
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Yuan, Weiyong Lu, Zhisong Wang, Huili Li, Chang Ming Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure |
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Yuan, Weiyong |
title |
Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure |
title_short |
Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure |
title_full |
Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure |
title_fullStr |
Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure |
title_full_unstemmed |
Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure |
title_sort |
stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure |
publishDate |
2013 |
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https://hdl.handle.net/10356/97252 http://hdl.handle.net/10220/10429 |
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1681044534691102720 |