Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure

A unique delivery system to reversibly and controllably load and release proteins under physiological conditions is desirable for protein therapeutics. We fabricate an ultrafast exponentially growing nanoporous multilayer structure comprised of two weak polyelectrolytes, poly(ethyleneimine) and algi...

Full description

Saved in:
Bibliographic Details
Main Authors: Yuan, Weiyong, Lu, Zhisong, Wang, Huili, Li, Chang Ming
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/97252
http://hdl.handle.net/10220/10429
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-97252
record_format dspace
spelling sg-ntu-dr.10356-972522020-03-07T11:40:21Z Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure Yuan, Weiyong Lu, Zhisong Wang, Huili Li, Chang Ming School of Chemical and Biomedical Engineering School of Mechanical and Aerospace Engineering Centre for Advanced Bionanosystems A unique delivery system to reversibly and controllably load and release proteins under physiological conditions is desirable for protein therapeutics. We fabricate an ultrafast exponentially growing nanoporous multilayer structure comprised of two weak polyelectrolytes, poly(ethyleneimine) and alginate with thickness and chemical composition controlled by the assembly pH. For the first time, the assembled multilayered structure demonstrates stimuli-free reversible protein loading and release capability at physiological conditions by a synthetic material. The protein loading and release time can also be controlled by the assembled bilayer number. The highest loading capacity for the target protein and longest release time of proteins for layer-by-layer films reported to date have been achieved with a 15-bilayered film fabricated in this work. The prominent properties of the assembled film provide great potential for various biomedical applications, especially as a delivery system for protein therapeutics. 2013-06-17T04:07:51Z 2019-12-06T19:40:36Z 2013-06-17T04:07:51Z 2019-12-06T19:40:36Z 2012 2012 Journal Article Yuan, W., Lu, Z., Wang, H., & Li, C. M. (2012). Stimuli-Free Reversible and Controllable Loading and Release of Proteins under Physiological Conditions by Exponentially Growing Nanoporous Multilayered Structure. Advanced Functional Materials, 22(9), 1932-1939. 1616-3028 https://hdl.handle.net/10356/97252 http://hdl.handle.net/10220/10429 10.1002/adfm.201102308 en Advanced functional materials © 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
description A unique delivery system to reversibly and controllably load and release proteins under physiological conditions is desirable for protein therapeutics. We fabricate an ultrafast exponentially growing nanoporous multilayer structure comprised of two weak polyelectrolytes, poly(ethyleneimine) and alginate with thickness and chemical composition controlled by the assembly pH. For the first time, the assembled multilayered structure demonstrates stimuli-free reversible protein loading and release capability at physiological conditions by a synthetic material. The protein loading and release time can also be controlled by the assembled bilayer number. The highest loading capacity for the target protein and longest release time of proteins for layer-by-layer films reported to date have been achieved with a 15-bilayered film fabricated in this work. The prominent properties of the assembled film provide great potential for various biomedical applications, especially as a delivery system for protein therapeutics.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Yuan, Weiyong
Lu, Zhisong
Wang, Huili
Li, Chang Ming
format Article
author Yuan, Weiyong
Lu, Zhisong
Wang, Huili
Li, Chang Ming
spellingShingle Yuan, Weiyong
Lu, Zhisong
Wang, Huili
Li, Chang Ming
Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure
author_sort Yuan, Weiyong
title Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure
title_short Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure
title_full Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure
title_fullStr Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure
title_full_unstemmed Stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure
title_sort stimuli-free reversible and controllable loading and release of proteins under physiological conditions by exponentially growing nanoporous multilayered structure
publishDate 2013
url https://hdl.handle.net/10356/97252
http://hdl.handle.net/10220/10429
_version_ 1681044534691102720