Bioconjugation of luminescent silicon quantum dots to gadolinium ions for bioimaging applications
Luminescent imaging agents and MRI contrast agents are desirable components in the rational design of multifunctional nanoconstructs for biological imaging applications. Luminescent biocompatible silicon quantum dots (SiQDs) and gadolinium chelates can be applied for fluorescence microscopy and MRI,...
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sg-ntu-dr.10356-974812020-03-07T14:02:47Z Bioconjugation of luminescent silicon quantum dots to gadolinium ions for bioimaging applications May, Jasmine L. Swihart, Mark T. Prasad, Paras N. Erogbogbo, Folarin Chang, Ching-Wen Liu, Liwei Kumar, Rajiv Law, Wing-Cheung Ding, Hong Yong, Ken-Tye Roy, Indrajit Sheshadri, Mukund School of Electrical and Electronic Engineering DRNTU::Engineering::Electrical and electronic engineering Luminescent imaging agents and MRI contrast agents are desirable components in the rational design of multifunctional nanoconstructs for biological imaging applications. Luminescent biocompatible silicon quantum dots (SiQDs) and gadolinium chelates can be applied for fluorescence microscopy and MRI, respectively. Here, we report the first synthesis of a nanocomplex incorporating SiQDs and gadolinium ions (Gd3+) for biological applications. The nanoconstruct is composed of a PEGylated micelle, with hydrophobic SiQDs in its core, covalently bound to DOTA-chelated Gd3+. Dynamic light scattering reveals a radius of 85 nm for these nanoconstructs, which is consistent with the electron microscopy results depicting radii ranging from 25 to 60 nm. Cellular uptake of the probes verified that they maintain their optical properties within the intracellular environment. The magnetic resonance relaxivity of the nanoconstruct was 2.4 mM−1 s−1 (in terms of Gd3+ concentration), calculated to be around 6000 mM−1 s−1 per nanoconstruct. These desirable optical and relaxivity properties of the newly developed probe open the door for use of SiQDs in future multimodal applications such as tumour imaging. 2013-06-26T07:24:21Z 2019-12-06T19:43:11Z 2013-06-26T07:24:21Z 2019-12-06T19:43:11Z 2012 2012 Journal Article Erogbogbo, F., Chang, C.-W., May, J. L., Liu, L., Kumar, R., Law, W.-C., et al. (2012). Bioconjugation of luminescent silicon quantum dots to gadolinium ions for bioimaging applications. Nanoscale, 4(17), 5483-5489. 2040-3364 https://hdl.handle.net/10356/97481 http://hdl.handle.net/10220/10724 10.1039/c2nr31002c en Nanoscale © 2012 The Royal Society of Chemistry. |
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DRNTU::Engineering::Electrical and electronic engineering May, Jasmine L. Swihart, Mark T. Prasad, Paras N. Erogbogbo, Folarin Chang, Ching-Wen Liu, Liwei Kumar, Rajiv Law, Wing-Cheung Ding, Hong Yong, Ken-Tye Roy, Indrajit Sheshadri, Mukund Bioconjugation of luminescent silicon quantum dots to gadolinium ions for bioimaging applications |
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Luminescent imaging agents and MRI contrast agents are desirable components in the rational design of multifunctional nanoconstructs for biological imaging applications. Luminescent biocompatible silicon quantum dots (SiQDs) and gadolinium chelates can be applied for fluorescence microscopy and MRI, respectively. Here, we report the first synthesis of a nanocomplex incorporating SiQDs and gadolinium ions (Gd3+) for biological applications. The nanoconstruct is composed of a PEGylated micelle, with hydrophobic SiQDs in its core, covalently bound to DOTA-chelated Gd3+. Dynamic light scattering reveals a radius of 85 nm for these nanoconstructs, which is consistent with the electron microscopy results depicting radii ranging from 25 to 60 nm. Cellular uptake of the probes verified that they maintain their optical properties within the intracellular environment. The magnetic resonance relaxivity of the nanoconstruct was 2.4 mM−1 s−1 (in terms of Gd3+ concentration), calculated to be around 6000 mM−1 s−1 per nanoconstruct. These desirable optical and relaxivity properties of the newly developed probe open the door for use of SiQDs in future multimodal applications such as tumour imaging. |
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School of Electrical and Electronic Engineering |
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School of Electrical and Electronic Engineering May, Jasmine L. Swihart, Mark T. Prasad, Paras N. Erogbogbo, Folarin Chang, Ching-Wen Liu, Liwei Kumar, Rajiv Law, Wing-Cheung Ding, Hong Yong, Ken-Tye Roy, Indrajit Sheshadri, Mukund |
format |
Article |
author |
May, Jasmine L. Swihart, Mark T. Prasad, Paras N. Erogbogbo, Folarin Chang, Ching-Wen Liu, Liwei Kumar, Rajiv Law, Wing-Cheung Ding, Hong Yong, Ken-Tye Roy, Indrajit Sheshadri, Mukund |
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May, Jasmine L. |
title |
Bioconjugation of luminescent silicon quantum dots to gadolinium ions for bioimaging applications |
title_short |
Bioconjugation of luminescent silicon quantum dots to gadolinium ions for bioimaging applications |
title_full |
Bioconjugation of luminescent silicon quantum dots to gadolinium ions for bioimaging applications |
title_fullStr |
Bioconjugation of luminescent silicon quantum dots to gadolinium ions for bioimaging applications |
title_full_unstemmed |
Bioconjugation of luminescent silicon quantum dots to gadolinium ions for bioimaging applications |
title_sort |
bioconjugation of luminescent silicon quantum dots to gadolinium ions for bioimaging applications |
publishDate |
2013 |
url |
https://hdl.handle.net/10356/97481 http://hdl.handle.net/10220/10724 |
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