Local electrical potential detection of DNA by nanowire–nanopore sensors

Nanopores could potentially be used to perform single-molecule DNA sequencing at low cost and with high throughput1, 2, 3, 4. Although single base resolution and differentiation have been demonstrated with nanopores using ionic current measurements5, 6, 7, direct sequencing has not been achieved bec...

Full description

Saved in:
Bibliographic Details
Main Authors: Lieber, Charles M., Xie, Ping, Xiong, Qihua, Fang, Ying, Qing, Quan
Other Authors: School of Electrical and Electronic Engineering
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/97516
http://hdl.handle.net/10220/10623
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-97516
record_format dspace
spelling sg-ntu-dr.10356-975162022-02-16T16:31:27Z Local electrical potential detection of DNA by nanowire–nanopore sensors Lieber, Charles M. Xie, Ping Xiong, Qihua Fang, Ying Qing, Quan School of Electrical and Electronic Engineering School of Physical and Mathematical Sciences Nanopores could potentially be used to perform single-molecule DNA sequencing at low cost and with high throughput1, 2, 3, 4. Although single base resolution and differentiation have been demonstrated with nanopores using ionic current measurements5, 6, 7, direct sequencing has not been achieved because of the difficulties in recording very small (~pA) ionic currents at a bandwidth consistent with fast translocation speeds1, 2, 3. Here, we show that solid-state nanopores can be combined with silicon nanowire field-effect transistors to create sensors in which detection is localized and self-aligned at the nanopore. Well-defined field-effect transistor signals associated with DNA translocation are recorded when an ionic strength gradient is imposed across the nanopores. Measurements and modelling show that field-effect transistor signals are generated by highly localized changes in the electrical potential during DNA translocation, and that nanowire–nanopore sensors could enable large-scale integration with a high intrinsic bandwidth. 2013-06-25T06:02:47Z 2019-12-06T19:43:29Z 2013-06-25T06:02:47Z 2019-12-06T19:43:29Z 2012 2012 Journal Article Xie, P., Xiong, Q., Fang, Y., Qing, Q., & Lieber, C. M. (2012). Local electrical potential detection of DNA by nanowire–nanopore sensors. Nature Nanotechnology, 7(2), 119-125. 1748-3387 https://hdl.handle.net/10356/97516 http://hdl.handle.net/10220/10623 10.1038/nnano.2011.217 22157724 en Nature nanotechnology © 2012 Macmillan Publishers Limited.
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
description Nanopores could potentially be used to perform single-molecule DNA sequencing at low cost and with high throughput1, 2, 3, 4. Although single base resolution and differentiation have been demonstrated with nanopores using ionic current measurements5, 6, 7, direct sequencing has not been achieved because of the difficulties in recording very small (~pA) ionic currents at a bandwidth consistent with fast translocation speeds1, 2, 3. Here, we show that solid-state nanopores can be combined with silicon nanowire field-effect transistors to create sensors in which detection is localized and self-aligned at the nanopore. Well-defined field-effect transistor signals associated with DNA translocation are recorded when an ionic strength gradient is imposed across the nanopores. Measurements and modelling show that field-effect transistor signals are generated by highly localized changes in the electrical potential during DNA translocation, and that nanowire–nanopore sensors could enable large-scale integration with a high intrinsic bandwidth.
author2 School of Electrical and Electronic Engineering
author_facet School of Electrical and Electronic Engineering
Lieber, Charles M.
Xie, Ping
Xiong, Qihua
Fang, Ying
Qing, Quan
format Article
author Lieber, Charles M.
Xie, Ping
Xiong, Qihua
Fang, Ying
Qing, Quan
spellingShingle Lieber, Charles M.
Xie, Ping
Xiong, Qihua
Fang, Ying
Qing, Quan
Local electrical potential detection of DNA by nanowire–nanopore sensors
author_sort Lieber, Charles M.
title Local electrical potential detection of DNA by nanowire–nanopore sensors
title_short Local electrical potential detection of DNA by nanowire–nanopore sensors
title_full Local electrical potential detection of DNA by nanowire–nanopore sensors
title_fullStr Local electrical potential detection of DNA by nanowire–nanopore sensors
title_full_unstemmed Local electrical potential detection of DNA by nanowire–nanopore sensors
title_sort local electrical potential detection of dna by nanowire–nanopore sensors
publishDate 2013
url https://hdl.handle.net/10356/97516
http://hdl.handle.net/10220/10623
_version_ 1725985581444366336