Axicon lens-based cone shell configuration for depth-sensitive fluorescence measurements in turbid media
We have developed a novel noncontact setup to implement a cone shell illumination and detection configuration using axicon lenses for depth-sensitive fluorescence measurements. The setup was demonstrated experimentally to be capable of detecting fluorescence from a two-layered turbid agar phantom wi...
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| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/97776 http://hdl.handle.net/10220/13226 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | We have developed a novel noncontact setup to implement a cone shell illumination and detection configuration using axicon lenses for depth-sensitive fluorescence measurements. The setup was demonstrated experimentally to be capable of detecting fluorescence from a two-layered turbid agar phantom with a larger sensitivity to the deep layer and a larger range of sensitivity to either layer than a conventional cone configuration implemented by a microscope objective lens. Furthermore, the axicon lens-based setup eliminates the need of moving the objective lens up or down to achieve depth-sensitive measurements, which effectively improves the consistency of optical coupling and thus would be preferred in a clinical setting. |
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