Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish

In zebrafish, as in many animals, maternal dorsal determinants are vegetally localized in the egg and are transported after fertilization in a microtubule-dependent manner. However, the organization of early microtubules, their dynamics and their contribution to axis formation are not fully understo...

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Main Authors: Shindo, A., Mimori-Kiyosue, Y., Mione, M., Hino, H., Quach, H., Lim, S., Tran, L. D., Ueno, N., Hibi, M., Sampath, Karuna., Winkler, Christoph.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/97790
http://hdl.handle.net/10220/13217
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-977902020-03-07T12:18:15Z Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish Shindo, A. Mimori-Kiyosue, Y. Mione, M. Hino, H. Quach, H. Lim, S. Tran, L. D. Ueno, N. Hibi, M. Sampath, Karuna. Winkler, Christoph. School of Biological Sciences DRNTU::Science::Biological sciences In zebrafish, as in many animals, maternal dorsal determinants are vegetally localized in the egg and are transported after fertilization in a microtubule-dependent manner. However, the organization of early microtubules, their dynamics and their contribution to axis formation are not fully understood. Using live imaging, we identified two populations of microtubules, perpendicular bundles and parallel arrays, which are directionally oriented and detected exclusively at the vegetal cortex before the first cell division. Perpendicular bundles emanate from the vegetal cortex, extend towards the blastoderm, and orient along the animal-vegetal axis. Parallel arrays become asymmetric on the vegetal cortex, and orient towards dorsal. We show that the orientation of microtubules at 20 minutes post-fertilization can predict where the embryonic dorsal structures in zebrafish will form. Furthermore, we find that parallel microtubule arrays colocalize with wnt8a RNA, the candidate maternal dorsal factor. Vegetal cytoplasmic granules are displaced with parallel arrays by ∼20°, providing in vivo evidence of a cortical rotation-like process in zebrafish. Cortical displacement requires parallel microtubule arrays, and probably contributes to asymmetric transport of maternal determinants. Formation of parallel arrays depends on Ca2+ signaling. Thus, microtubule polarity and organization predicts the zebrafish embryonic axis. In addition, our results suggest that cortical rotation-like processes might be more common in early development than previously thought. 2013-08-26T06:16:15Z 2019-12-06T19:46:46Z 2013-08-26T06:16:15Z 2019-12-06T19:46:46Z 2012 2012 Journal Article Tran, L. D., Hino, H., Quach, H., Lim, S., Shindo, A., Mimori-Kiyosue, Y., Mione, M., Ueno, N., Winkler, C., Hibi, M.,& Sampath, K. (2012). Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish. Development, 139(19), 3644-3652. https://hdl.handle.net/10356/97790 http://hdl.handle.net/10220/13217 10.1242/dev.082362 en Development
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Shindo, A.
Mimori-Kiyosue, Y.
Mione, M.
Hino, H.
Quach, H.
Lim, S.
Tran, L. D.
Ueno, N.
Hibi, M.
Sampath, Karuna.
Winkler, Christoph.
Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish
description In zebrafish, as in many animals, maternal dorsal determinants are vegetally localized in the egg and are transported after fertilization in a microtubule-dependent manner. However, the organization of early microtubules, their dynamics and their contribution to axis formation are not fully understood. Using live imaging, we identified two populations of microtubules, perpendicular bundles and parallel arrays, which are directionally oriented and detected exclusively at the vegetal cortex before the first cell division. Perpendicular bundles emanate from the vegetal cortex, extend towards the blastoderm, and orient along the animal-vegetal axis. Parallel arrays become asymmetric on the vegetal cortex, and orient towards dorsal. We show that the orientation of microtubules at 20 minutes post-fertilization can predict where the embryonic dorsal structures in zebrafish will form. Furthermore, we find that parallel microtubule arrays colocalize with wnt8a RNA, the candidate maternal dorsal factor. Vegetal cytoplasmic granules are displaced with parallel arrays by ∼20°, providing in vivo evidence of a cortical rotation-like process in zebrafish. Cortical displacement requires parallel microtubule arrays, and probably contributes to asymmetric transport of maternal determinants. Formation of parallel arrays depends on Ca2+ signaling. Thus, microtubule polarity and organization predicts the zebrafish embryonic axis. In addition, our results suggest that cortical rotation-like processes might be more common in early development than previously thought.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Shindo, A.
Mimori-Kiyosue, Y.
Mione, M.
Hino, H.
Quach, H.
Lim, S.
Tran, L. D.
Ueno, N.
Hibi, M.
Sampath, Karuna.
Winkler, Christoph.
format Article
author Shindo, A.
Mimori-Kiyosue, Y.
Mione, M.
Hino, H.
Quach, H.
Lim, S.
Tran, L. D.
Ueno, N.
Hibi, M.
Sampath, Karuna.
Winkler, Christoph.
author_sort Shindo, A.
title Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish
title_short Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish
title_full Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish
title_fullStr Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish
title_full_unstemmed Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish
title_sort dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish
publishDate 2013
url https://hdl.handle.net/10356/97790
http://hdl.handle.net/10220/13217
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