Characterization of extracellular lignocellulolytic enzymes of Coniochaeta sp. during corn stover bioconversion

• Main Authors: Ravindran, Anita, Sze, Siu Kwan, Adav, Sunil S.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/97943; http://hdl.handle.net/10220/12318
• Institution: Nanyang Technological University

Biorefinery of renewable lignocellulosic biomass to biochemical and biofuel is a promising technology to mitigate global warming and fuel shortage but hydrolysis of recalcitrant lignocellulose to its constitutive components is the bottleneck of the process. This work isolated and characterized a new lignocellulose degrading filamentous fungus from decomposing wood in mangrove area. The strain was identified as Coniochaeta sp. according to ITS rRNA sequences and its phylogenic analysis. The extracellular lignocellulolytic enzymes of this fungal strain, when grown on corn stover, were profiled by LC–MS/MS and exponentially modified protein abundance index (emPAI) based label-free quantitative proteomics approach. We identified 107 potential lignocellulolytic enzymes and their functional classification revealed unique extracellular enzyme system constituting multienzyme complexes of cellulases (29%), hemicellulases (17%), glycoside hydrolases (10%), proteases and peptidases (24%), lignin degrading enzymes (7%) and hypothetical proteins (13%). The growth behavior, biochemical assay and LC–MS/MS analysis of secretome by isolated fungal strain revealed its lignocellulose degradation potential when cultivated with corn stover as a major carbon source.