Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell
The bioelectrocatalysis in microbial fuel cells (MFCs) relies on both electrochemistry and metabolism of microbes. We discovered that under MFC microaerobic condition, an arcA knockout mutant Escherichia coli (arcA–) shows enhanced activation of the citric acid cycle (TCA cycle) for glycerol oxidati...
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sg-ntu-dr.10356-979552020-03-07T11:35:36Z Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell Liu, Jing Yong, Yang-Chun Song, Hao Li, Chang Ming School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology The bioelectrocatalysis in microbial fuel cells (MFCs) relies on both electrochemistry and metabolism of microbes. We discovered that under MFC microaerobic condition, an arcA knockout mutant Escherichia coli (arcA–) shows enhanced activation of the citric acid cycle (TCA cycle) for glycerol oxidation, as indicated by the increased key enzymes’ activity in the TCA cycle. Meanwhile, a diffusive electron mediator (hydroxyl quinone derivative) is excreted by the genetically engineered arcA–, resulting in a much higher power density than its parental strain toward glycerol oxidation. This work demonstrates that metabolic engineering is a feasible approach to construct efficient bioelectrocatalysts for high-performance MFCs. 2013-07-25T07:14:10Z 2019-12-06T19:48:44Z 2013-07-25T07:14:10Z 2019-12-06T19:48:44Z 2012 2012 Journal Article Liu, J., Yong, Y.-C., Song, H., & Li, C. M. (2012). Activation Enhancement of Citric Acid Cycle to Promote Bioelectrocatalytic Activity of arcA Knockout Escherichia coli Toward High-Performance Microbial Fuel Cell. ACS Catalysis, 2(8), 1749-1752. https://hdl.handle.net/10356/97955 http://hdl.handle.net/10220/12260 10.1021/cs3003808 179815 en ACS Catalysis © 2012 American chemical society. |
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DRNTU::Engineering::Chemical engineering::Biotechnology Liu, Jing Yong, Yang-Chun Song, Hao Li, Chang Ming Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell |
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The bioelectrocatalysis in microbial fuel cells (MFCs) relies on both electrochemistry and metabolism of microbes. We discovered that under MFC microaerobic condition, an arcA knockout mutant Escherichia coli (arcA–) shows enhanced activation of the citric acid cycle (TCA cycle) for glycerol oxidation, as indicated by the increased key enzymes’ activity in the TCA cycle. Meanwhile, a diffusive electron mediator (hydroxyl quinone derivative) is excreted by the genetically engineered arcA–, resulting in a much higher power density than its parental strain toward glycerol oxidation. This work demonstrates that metabolic engineering is a feasible approach to construct efficient bioelectrocatalysts for high-performance MFCs. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Liu, Jing Yong, Yang-Chun Song, Hao Li, Chang Ming |
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Article |
author |
Liu, Jing Yong, Yang-Chun Song, Hao Li, Chang Ming |
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Liu, Jing |
title |
Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell |
title_short |
Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell |
title_full |
Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell |
title_fullStr |
Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell |
title_full_unstemmed |
Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell |
title_sort |
activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arca knockout escherichia coli toward high-performance microbial fuel cell |
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2013 |
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https://hdl.handle.net/10356/97955 http://hdl.handle.net/10220/12260 |
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