Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell

The bioelectrocatalysis in microbial fuel cells (MFCs) relies on both electrochemistry and metabolism of microbes. We discovered that under MFC microaerobic condition, an arcA knockout mutant Escherichia coli (arcA–) shows enhanced activation of the citric acid cycle (TCA cycle) for glycerol oxidati...

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Main Authors: Liu, Jing, Yong, Yang-Chun, Song, Hao, Li, Chang Ming
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/97955
http://hdl.handle.net/10220/12260
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-979552020-03-07T11:35:36Z Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell Liu, Jing Yong, Yang-Chun Song, Hao Li, Chang Ming School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biotechnology The bioelectrocatalysis in microbial fuel cells (MFCs) relies on both electrochemistry and metabolism of microbes. We discovered that under MFC microaerobic condition, an arcA knockout mutant Escherichia coli (arcA–) shows enhanced activation of the citric acid cycle (TCA cycle) for glycerol oxidation, as indicated by the increased key enzymes’ activity in the TCA cycle. Meanwhile, a diffusive electron mediator (hydroxyl quinone derivative) is excreted by the genetically engineered arcA–, resulting in a much higher power density than its parental strain toward glycerol oxidation. This work demonstrates that metabolic engineering is a feasible approach to construct efficient bioelectrocatalysts for high-performance MFCs. 2013-07-25T07:14:10Z 2019-12-06T19:48:44Z 2013-07-25T07:14:10Z 2019-12-06T19:48:44Z 2012 2012 Journal Article Liu, J., Yong, Y.-C., Song, H., & Li, C. M. (2012). Activation Enhancement of Citric Acid Cycle to Promote Bioelectrocatalytic Activity of arcA Knockout Escherichia coli Toward High-Performance Microbial Fuel Cell. ACS Catalysis, 2(8), 1749-1752. https://hdl.handle.net/10356/97955 http://hdl.handle.net/10220/12260 10.1021/cs3003808 179815 en ACS Catalysis © 2012 American chemical society.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Chemical engineering::Biotechnology
spellingShingle DRNTU::Engineering::Chemical engineering::Biotechnology
Liu, Jing
Yong, Yang-Chun
Song, Hao
Li, Chang Ming
Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell
description The bioelectrocatalysis in microbial fuel cells (MFCs) relies on both electrochemistry and metabolism of microbes. We discovered that under MFC microaerobic condition, an arcA knockout mutant Escherichia coli (arcA–) shows enhanced activation of the citric acid cycle (TCA cycle) for glycerol oxidation, as indicated by the increased key enzymes’ activity in the TCA cycle. Meanwhile, a diffusive electron mediator (hydroxyl quinone derivative) is excreted by the genetically engineered arcA–, resulting in a much higher power density than its parental strain toward glycerol oxidation. This work demonstrates that metabolic engineering is a feasible approach to construct efficient bioelectrocatalysts for high-performance MFCs.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Liu, Jing
Yong, Yang-Chun
Song, Hao
Li, Chang Ming
format Article
author Liu, Jing
Yong, Yang-Chun
Song, Hao
Li, Chang Ming
author_sort Liu, Jing
title Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell
title_short Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell
title_full Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell
title_fullStr Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell
title_full_unstemmed Activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arcA knockout Escherichia coli toward high-performance microbial fuel cell
title_sort activation enhancement of citric acid cycle to promote bioelectrocatalytic activity of arca knockout escherichia coli toward high-performance microbial fuel cell
publishDate 2013
url https://hdl.handle.net/10356/97955
http://hdl.handle.net/10220/12260
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