Oxidation of DNA bases influenced by the presence of other bases

Electrochemical detection of DNA is a highly important topic. Here we show that the electrochemical responses of one DNA base (guanine, adenine, cytosine or thymine), in terms of oxidation potential, current intensity, peak width and resolution can be highly influenced by the presence of other DNA...

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Main Authors: Shuhua Goh, Madeline, Pumera, Martin
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/98173
http://hdl.handle.net/10220/13258
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-981732020-03-07T12:34:44Z Oxidation of DNA bases influenced by the presence of other bases Shuhua Goh, Madeline Pumera, Martin School of Physical and Mathematical Sciences Electrochemical detection of DNA is a highly important topic. Here we show that the electrochemical responses of one DNA base (guanine, adenine, cytosine or thymine), in terms of oxidation potential, current intensity, peak width and resolution can be highly influenced by the presence of other DNA bases at electrochemically reduced graphene oxide (ER-GO) as well as standard glassy carbon electrode. We have observed that the effects were more significant for adenine base on ER-GO and cytosine base on glassy carbon (GC) electrode. Differences in responses were generally low in a mixture of four different DNA bases but interestingly, deviations become significantly larger when only one or two other bases were present. Our findings are of paramount importance for future developments in DNA detection and analysis since individual DNA bases are not present in isolation in nature or in typical biosensing systems. 2013-08-29T07:23:51Z 2019-12-06T19:51:46Z 2013-08-29T07:23:51Z 2019-12-06T19:51:46Z 2012 2012 Journal Article Shuhua Goh, M.,& Pumera, M. (2012). Oxidation of DNA Bases Influenced by the Presence of Other Bases. Electroanalysis, 24(5), 1147-1152. 1040-0397 https://hdl.handle.net/10356/98173 http://hdl.handle.net/10220/13258 10.1002/elan.201200036 en Electroanalysis
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
description Electrochemical detection of DNA is a highly important topic. Here we show that the electrochemical responses of one DNA base (guanine, adenine, cytosine or thymine), in terms of oxidation potential, current intensity, peak width and resolution can be highly influenced by the presence of other DNA bases at electrochemically reduced graphene oxide (ER-GO) as well as standard glassy carbon electrode. We have observed that the effects were more significant for adenine base on ER-GO and cytosine base on glassy carbon (GC) electrode. Differences in responses were generally low in a mixture of four different DNA bases but interestingly, deviations become significantly larger when only one or two other bases were present. Our findings are of paramount importance for future developments in DNA detection and analysis since individual DNA bases are not present in isolation in nature or in typical biosensing systems.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Shuhua Goh, Madeline
Pumera, Martin
format Article
author Shuhua Goh, Madeline
Pumera, Martin
spellingShingle Shuhua Goh, Madeline
Pumera, Martin
Oxidation of DNA bases influenced by the presence of other bases
author_sort Shuhua Goh, Madeline
title Oxidation of DNA bases influenced by the presence of other bases
title_short Oxidation of DNA bases influenced by the presence of other bases
title_full Oxidation of DNA bases influenced by the presence of other bases
title_fullStr Oxidation of DNA bases influenced by the presence of other bases
title_full_unstemmed Oxidation of DNA bases influenced by the presence of other bases
title_sort oxidation of dna bases influenced by the presence of other bases
publishDate 2013
url https://hdl.handle.net/10356/98173
http://hdl.handle.net/10220/13258
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