Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass
Solid state fermentation of lignocellulosic biomass by filamentous microorganisms to induced enzyme production has been recognized as an attractive and cost effective technology. The secretion profile of lignocellulolytic enzymes by thermostable filamentous Thermobifida fusca (T. fusca) in solid sta...
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sg-ntu-dr.10356-985822020-03-07T12:18:06Z Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass Ravindran, Anita Dutta, Bamaprasad Sze, Siu Kwan Cheow, Esther Sok Hwee Adav, Sunil S. School of Biological Sciences DRNTU::Science::Biological sciences Solid state fermentation of lignocellulosic biomass by filamentous microorganisms to induced enzyme production has been recognized as an attractive and cost effective technology. The secretion profile of lignocellulolytic enzymes by thermostable filamentous Thermobifida fusca (T. fusca) in solid state fermentation of different lignocellulosic biomasses, such as corn stover, hay; saw dust; sugarcane bagasse; wood chips; and un-dried green plant were explored using label-free exponentially modified protein abundance index (emPAI) based quantitative proteomics. Comparative analyses of T. fusca secretion profiles between cellulose and the various lignocellulosic biomasses showed induced expression of cellulolytic proteins by cellulose, and expression of hemicellulose, pectin and lignin degrading enzymes were induced by lignocellulosic biomasses. The solid state fermentation by T. fusca on lignocellulosic biomasses also revealed increased expressions of various transport proteins and hypothetical proteins. The Bray–Curtis similarity indices, clustering, and multidimensional scaling plot explicated differential protein expressions by T. fusca on different lignocellulosic biomasses, indicating that protein secretion by T. fusca is reliant on substrate complexity. 2013-09-24T07:45:23Z 2019-12-06T19:57:05Z 2013-09-24T07:45:23Z 2019-12-06T19:57:05Z 2012 2012 Journal Article Adav, S. S., Cheow, E. S. H., Ravindran, A., Dutta, B., & Sze, S. K. (2012). Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass. Journal of Proteomics, 75(12), 3694–3706. https://hdl.handle.net/10356/98582 http://hdl.handle.net/10220/13667 10.1016/j.jprot.2012.04.031 en Journal of proteomics |
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DRNTU::Science::Biological sciences Ravindran, Anita Dutta, Bamaprasad Sze, Siu Kwan Cheow, Esther Sok Hwee Adav, Sunil S. Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass |
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Solid state fermentation of lignocellulosic biomass by filamentous microorganisms to induced enzyme production has been recognized as an attractive and cost effective technology. The secretion profile of lignocellulolytic enzymes by thermostable filamentous Thermobifida fusca (T. fusca) in solid state fermentation of different lignocellulosic biomasses, such as corn stover, hay; saw dust; sugarcane bagasse; wood chips; and un-dried green plant were explored using label-free exponentially modified protein abundance index (emPAI) based quantitative proteomics. Comparative analyses of T. fusca secretion profiles between cellulose and the various lignocellulosic biomasses showed induced expression of cellulolytic proteins by cellulose, and expression of hemicellulose, pectin and lignin degrading enzymes were induced by lignocellulosic biomasses. The solid state fermentation by T. fusca on lignocellulosic biomasses also revealed increased expressions of various transport proteins and hypothetical proteins. The Bray–Curtis similarity indices, clustering, and multidimensional scaling plot explicated differential protein expressions by T. fusca on different lignocellulosic biomasses, indicating that protein secretion by T. fusca is reliant on substrate complexity. |
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School of Biological Sciences |
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School of Biological Sciences Ravindran, Anita Dutta, Bamaprasad Sze, Siu Kwan Cheow, Esther Sok Hwee Adav, Sunil S. |
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Article |
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Ravindran, Anita Dutta, Bamaprasad Sze, Siu Kwan Cheow, Esther Sok Hwee Adav, Sunil S. |
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Ravindran, Anita |
title |
Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass |
title_short |
Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass |
title_full |
Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass |
title_fullStr |
Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass |
title_full_unstemmed |
Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass |
title_sort |
label free quantitative proteomic analysis of secretome by thermobifida fusca on different lignocellulosic biomass |
publishDate |
2013 |
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https://hdl.handle.net/10356/98582 http://hdl.handle.net/10220/13667 |
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