Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass

Solid state fermentation of lignocellulosic biomass by filamentous microorganisms to induced enzyme production has been recognized as an attractive and cost effective technology. The secretion profile of lignocellulolytic enzymes by thermostable filamentous Thermobifida fusca (T. fusca) in solid sta...

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Main Authors: Ravindran, Anita, Dutta, Bamaprasad, Sze, Siu Kwan, Cheow, Esther Sok Hwee, Adav, Sunil S.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/98582
http://hdl.handle.net/10220/13667
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-985822020-03-07T12:18:06Z Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass Ravindran, Anita Dutta, Bamaprasad Sze, Siu Kwan Cheow, Esther Sok Hwee Adav, Sunil S. School of Biological Sciences DRNTU::Science::Biological sciences Solid state fermentation of lignocellulosic biomass by filamentous microorganisms to induced enzyme production has been recognized as an attractive and cost effective technology. The secretion profile of lignocellulolytic enzymes by thermostable filamentous Thermobifida fusca (T. fusca) in solid state fermentation of different lignocellulosic biomasses, such as corn stover, hay; saw dust; sugarcane bagasse; wood chips; and un-dried green plant were explored using label-free exponentially modified protein abundance index (emPAI) based quantitative proteomics. Comparative analyses of T. fusca secretion profiles between cellulose and the various lignocellulosic biomasses showed induced expression of cellulolytic proteins by cellulose, and expression of hemicellulose, pectin and lignin degrading enzymes were induced by lignocellulosic biomasses. The solid state fermentation by T. fusca on lignocellulosic biomasses also revealed increased expressions of various transport proteins and hypothetical proteins. The Bray–Curtis similarity indices, clustering, and multidimensional scaling plot explicated differential protein expressions by T. fusca on different lignocellulosic biomasses, indicating that protein secretion by T. fusca is reliant on substrate complexity. 2013-09-24T07:45:23Z 2019-12-06T19:57:05Z 2013-09-24T07:45:23Z 2019-12-06T19:57:05Z 2012 2012 Journal Article Adav, S. S., Cheow, E. S. H., Ravindran, A., Dutta, B., & Sze, S. K. (2012). Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass. Journal of Proteomics, 75(12), 3694–3706. https://hdl.handle.net/10356/98582 http://hdl.handle.net/10220/13667 10.1016/j.jprot.2012.04.031 en Journal of proteomics
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Ravindran, Anita
Dutta, Bamaprasad
Sze, Siu Kwan
Cheow, Esther Sok Hwee
Adav, Sunil S.
Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass
description Solid state fermentation of lignocellulosic biomass by filamentous microorganisms to induced enzyme production has been recognized as an attractive and cost effective technology. The secretion profile of lignocellulolytic enzymes by thermostable filamentous Thermobifida fusca (T. fusca) in solid state fermentation of different lignocellulosic biomasses, such as corn stover, hay; saw dust; sugarcane bagasse; wood chips; and un-dried green plant were explored using label-free exponentially modified protein abundance index (emPAI) based quantitative proteomics. Comparative analyses of T. fusca secretion profiles between cellulose and the various lignocellulosic biomasses showed induced expression of cellulolytic proteins by cellulose, and expression of hemicellulose, pectin and lignin degrading enzymes were induced by lignocellulosic biomasses. The solid state fermentation by T. fusca on lignocellulosic biomasses also revealed increased expressions of various transport proteins and hypothetical proteins. The Bray–Curtis similarity indices, clustering, and multidimensional scaling plot explicated differential protein expressions by T. fusca on different lignocellulosic biomasses, indicating that protein secretion by T. fusca is reliant on substrate complexity.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Ravindran, Anita
Dutta, Bamaprasad
Sze, Siu Kwan
Cheow, Esther Sok Hwee
Adav, Sunil S.
format Article
author Ravindran, Anita
Dutta, Bamaprasad
Sze, Siu Kwan
Cheow, Esther Sok Hwee
Adav, Sunil S.
author_sort Ravindran, Anita
title Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass
title_short Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass
title_full Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass
title_fullStr Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass
title_full_unstemmed Label free quantitative proteomic analysis of secretome by Thermobifida fusca on different lignocellulosic biomass
title_sort label free quantitative proteomic analysis of secretome by thermobifida fusca on different lignocellulosic biomass
publishDate 2013
url https://hdl.handle.net/10356/98582
http://hdl.handle.net/10220/13667
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