‘Next-base’ effect on PCR amplification

• Main Authors: Ben-Dov, Eitan, Kushmaro, Ariel, Shapiro, Orr H.
• Other Authors: School of Materials Science & Engineering
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Engineering::Materials
• Online Access: https://hdl.handle.net/10356/98687; http://hdl.handle.net/10220/16237
• Institution: Nanyang Technological University

The base adjacent to the 3′ end of universal PCR primers targeting the 16S rRNA gene is often variable and apparently biases the microbial community composition as represented by PCR-based surveys. To test this hypothesis, four templates of 44 bases each and two complementary primers (21 bases) were...