Identification and evaluation of apoptotic compounds from Garcinia Oligantha

Four new compounds, oliganthins A–D (1–4), and one known caged xanthone gaudichaudione H (5) were isolated from the stems of Garcinia oligantha. The structures of the new compounds were elucidated by spectroscopic evidences. All of the five compounds were evaluated for their apoptosis-inducing effec...

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Main Authors: Han, Quan-Bin, Gao, Xue-Mei, Yu, Ting, Cui, Ming-Zhu, Pu, Jian-Xin, Du, Xue, Hu, Qiu-Fen, Liu, Tian-Cheng, Luo, Kathy Qian, Xu, Hong-Xi
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2013
Online Access:https://hdl.handle.net/10356/98986
http://hdl.handle.net/10220/12781
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-989862020-03-07T11:35:38Z Identification and evaluation of apoptotic compounds from Garcinia Oligantha Han, Quan-Bin Gao, Xue-Mei Yu, Ting Cui, Ming-Zhu Pu, Jian-Xin Du, Xue Hu, Qiu-Fen Liu, Tian-Cheng Luo, Kathy Qian Xu, Hong-Xi School of Chemical and Biomedical Engineering Four new compounds, oliganthins A–D (1–4), and one known caged xanthone gaudichaudione H (5) were isolated from the stems of Garcinia oligantha. The structures of the new compounds were elucidated by spectroscopic evidences. All of the five compounds were evaluated for their apoptosis-inducing effects using HeLa-C3 cells which have been genetically engineered to produce a fluorescent biosensor capable of detecting caspase-3 activation. All of them induced cell apoptosis at 10 μM or lower concentrations. The apoptotic activity of oliganthins A, B and gaudichaudione H were further confirmed by detecting the cleavage of PARP, which is the substrate of activated caspase-3, in these compounds-treated cells using the method of Western blot. Moreover, the values of IC50 were measured for all five compounds on HeLa cells using the MTT assay. Among them, gaudichaudione H had the lowest IC50 value of 0.90 μM, while the other four new compounds had IC50 values of 1.58, 1.52, 4.15, and 7.82 μM, respectively. These results show that gaudichaudione H has the strongest apoptosis-inducing effect and cell growth inhibition effect among these xanthones and it may have the potential to be developed into a new anticancer agent. 2013-08-01T04:25:07Z 2019-12-06T20:02:02Z 2013-08-01T04:25:07Z 2019-12-06T20:02:02Z 2012 2012 Journal Article Gao, X. M., Yu, T., Cui, M. z., Pu, J. X., Du, X., Han, Q. b., Hu, Q. f., Liu, T. C., Luo, K. Q.,& Xu, H. X. (2012). Identification and evaluation of apoptotic compounds from Garcinia oligantha. Bioorganic & Medicinal Chemistry Letters, 22(6), 2350-2353. 0960-894X https://hdl.handle.net/10356/98986 http://hdl.handle.net/10220/12781 10.1016/j.bmcl.2012.01.068 en Bioorganic & medicinal chemistry letters
institution Nanyang Technological University
building NTU Library
country Singapore
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description Four new compounds, oliganthins A–D (1–4), and one known caged xanthone gaudichaudione H (5) were isolated from the stems of Garcinia oligantha. The structures of the new compounds were elucidated by spectroscopic evidences. All of the five compounds were evaluated for their apoptosis-inducing effects using HeLa-C3 cells which have been genetically engineered to produce a fluorescent biosensor capable of detecting caspase-3 activation. All of them induced cell apoptosis at 10 μM or lower concentrations. The apoptotic activity of oliganthins A, B and gaudichaudione H were further confirmed by detecting the cleavage of PARP, which is the substrate of activated caspase-3, in these compounds-treated cells using the method of Western blot. Moreover, the values of IC50 were measured for all five compounds on HeLa cells using the MTT assay. Among them, gaudichaudione H had the lowest IC50 value of 0.90 μM, while the other four new compounds had IC50 values of 1.58, 1.52, 4.15, and 7.82 μM, respectively. These results show that gaudichaudione H has the strongest apoptosis-inducing effect and cell growth inhibition effect among these xanthones and it may have the potential to be developed into a new anticancer agent.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Han, Quan-Bin
Gao, Xue-Mei
Yu, Ting
Cui, Ming-Zhu
Pu, Jian-Xin
Du, Xue
Hu, Qiu-Fen
Liu, Tian-Cheng
Luo, Kathy Qian
Xu, Hong-Xi
format Article
author Han, Quan-Bin
Gao, Xue-Mei
Yu, Ting
Cui, Ming-Zhu
Pu, Jian-Xin
Du, Xue
Hu, Qiu-Fen
Liu, Tian-Cheng
Luo, Kathy Qian
Xu, Hong-Xi
spellingShingle Han, Quan-Bin
Gao, Xue-Mei
Yu, Ting
Cui, Ming-Zhu
Pu, Jian-Xin
Du, Xue
Hu, Qiu-Fen
Liu, Tian-Cheng
Luo, Kathy Qian
Xu, Hong-Xi
Identification and evaluation of apoptotic compounds from Garcinia Oligantha
author_sort Han, Quan-Bin
title Identification and evaluation of apoptotic compounds from Garcinia Oligantha
title_short Identification and evaluation of apoptotic compounds from Garcinia Oligantha
title_full Identification and evaluation of apoptotic compounds from Garcinia Oligantha
title_fullStr Identification and evaluation of apoptotic compounds from Garcinia Oligantha
title_full_unstemmed Identification and evaluation of apoptotic compounds from Garcinia Oligantha
title_sort identification and evaluation of apoptotic compounds from garcinia oligantha
publishDate 2013
url https://hdl.handle.net/10356/98986
http://hdl.handle.net/10220/12781
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