Identification and evaluation of apoptotic compounds from Garcinia Oligantha
Four new compounds, oliganthins A–D (1–4), and one known caged xanthone gaudichaudione H (5) were isolated from the stems of Garcinia oligantha. The structures of the new compounds were elucidated by spectroscopic evidences. All of the five compounds were evaluated for their apoptosis-inducing effec...
Saved in:
Main Authors: | , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2013
|
Online Access: | https://hdl.handle.net/10356/98986 http://hdl.handle.net/10220/12781 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-98986 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-989862020-03-07T11:35:38Z Identification and evaluation of apoptotic compounds from Garcinia Oligantha Han, Quan-Bin Gao, Xue-Mei Yu, Ting Cui, Ming-Zhu Pu, Jian-Xin Du, Xue Hu, Qiu-Fen Liu, Tian-Cheng Luo, Kathy Qian Xu, Hong-Xi School of Chemical and Biomedical Engineering Four new compounds, oliganthins A–D (1–4), and one known caged xanthone gaudichaudione H (5) were isolated from the stems of Garcinia oligantha. The structures of the new compounds were elucidated by spectroscopic evidences. All of the five compounds were evaluated for their apoptosis-inducing effects using HeLa-C3 cells which have been genetically engineered to produce a fluorescent biosensor capable of detecting caspase-3 activation. All of them induced cell apoptosis at 10 μM or lower concentrations. The apoptotic activity of oliganthins A, B and gaudichaudione H were further confirmed by detecting the cleavage of PARP, which is the substrate of activated caspase-3, in these compounds-treated cells using the method of Western blot. Moreover, the values of IC50 were measured for all five compounds on HeLa cells using the MTT assay. Among them, gaudichaudione H had the lowest IC50 value of 0.90 μM, while the other four new compounds had IC50 values of 1.58, 1.52, 4.15, and 7.82 μM, respectively. These results show that gaudichaudione H has the strongest apoptosis-inducing effect and cell growth inhibition effect among these xanthones and it may have the potential to be developed into a new anticancer agent. 2013-08-01T04:25:07Z 2019-12-06T20:02:02Z 2013-08-01T04:25:07Z 2019-12-06T20:02:02Z 2012 2012 Journal Article Gao, X. M., Yu, T., Cui, M. z., Pu, J. X., Du, X., Han, Q. b., Hu, Q. f., Liu, T. C., Luo, K. Q.,& Xu, H. X. (2012). Identification and evaluation of apoptotic compounds from Garcinia oligantha. Bioorganic & Medicinal Chemistry Letters, 22(6), 2350-2353. 0960-894X https://hdl.handle.net/10356/98986 http://hdl.handle.net/10220/12781 10.1016/j.bmcl.2012.01.068 en Bioorganic & medicinal chemistry letters |
institution |
Nanyang Technological University |
building |
NTU Library |
country |
Singapore |
collection |
DR-NTU |
language |
English |
description |
Four new compounds, oliganthins A–D (1–4), and one known caged xanthone gaudichaudione H (5) were isolated from the stems of Garcinia oligantha. The structures of the new compounds were elucidated by spectroscopic evidences. All of the five compounds were evaluated for their apoptosis-inducing effects using HeLa-C3 cells which have been genetically engineered to produce a fluorescent biosensor capable of detecting caspase-3 activation. All of them induced cell apoptosis at 10 μM or lower concentrations. The apoptotic activity of oliganthins A, B and gaudichaudione H were further confirmed by detecting the cleavage of PARP, which is the substrate of activated caspase-3, in these compounds-treated cells using the method of Western blot. Moreover, the values of IC50 were measured for all five compounds on HeLa cells using the MTT assay. Among them, gaudichaudione H had the lowest IC50 value of 0.90 μM, while the other four new compounds had IC50 values of 1.58, 1.52, 4.15, and 7.82 μM, respectively. These results show that gaudichaudione H has the strongest apoptosis-inducing effect and cell growth inhibition effect among these xanthones and it may have the potential to be developed into a new anticancer agent. |
author2 |
School of Chemical and Biomedical Engineering |
author_facet |
School of Chemical and Biomedical Engineering Han, Quan-Bin Gao, Xue-Mei Yu, Ting Cui, Ming-Zhu Pu, Jian-Xin Du, Xue Hu, Qiu-Fen Liu, Tian-Cheng Luo, Kathy Qian Xu, Hong-Xi |
format |
Article |
author |
Han, Quan-Bin Gao, Xue-Mei Yu, Ting Cui, Ming-Zhu Pu, Jian-Xin Du, Xue Hu, Qiu-Fen Liu, Tian-Cheng Luo, Kathy Qian Xu, Hong-Xi |
spellingShingle |
Han, Quan-Bin Gao, Xue-Mei Yu, Ting Cui, Ming-Zhu Pu, Jian-Xin Du, Xue Hu, Qiu-Fen Liu, Tian-Cheng Luo, Kathy Qian Xu, Hong-Xi Identification and evaluation of apoptotic compounds from Garcinia Oligantha |
author_sort |
Han, Quan-Bin |
title |
Identification and evaluation of apoptotic compounds from Garcinia Oligantha |
title_short |
Identification and evaluation of apoptotic compounds from Garcinia Oligantha |
title_full |
Identification and evaluation of apoptotic compounds from Garcinia Oligantha |
title_fullStr |
Identification and evaluation of apoptotic compounds from Garcinia Oligantha |
title_full_unstemmed |
Identification and evaluation of apoptotic compounds from Garcinia Oligantha |
title_sort |
identification and evaluation of apoptotic compounds from garcinia oligantha |
publishDate |
2013 |
url |
https://hdl.handle.net/10356/98986 http://hdl.handle.net/10220/12781 |
_version_ |
1681045681486168064 |