Thrombin aptasensing with inherently electroactive graphene oxide nanoplatelets as labels

Graphene and its associated materials are commonly used as the transducing platform in biosensing. We propose a different approach for the application of graphene in biosensing. Here, we utilized graphene oxide nanoplatelets as the inherently electroactive labels for the aptasensing of thrombin. The...

Full description

Saved in:
Bibliographic Details
Main Authors: Loo, Adeline Huiling, Bonanni, Alessandra, Pumera, Martin
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2013
Subjects:
Online Access:https://hdl.handle.net/10356/99155
http://hdl.handle.net/10220/17179
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-99155
record_format dspace
spelling sg-ntu-dr.10356-991552020-03-07T12:34:49Z Thrombin aptasensing with inherently electroactive graphene oxide nanoplatelets as labels Loo, Adeline Huiling Bonanni, Alessandra Pumera, Martin School of Physical and Mathematical Sciences DRNTU::Engineering::Nanotechnology Graphene and its associated materials are commonly used as the transducing platform in biosensing. We propose a different approach for the application of graphene in biosensing. Here, we utilized graphene oxide nanoplatelets as the inherently electroactive labels for the aptasensing of thrombin. The basis of detection lies in the ability of graphene oxide to be electrochemically reduced, thereby providing a well-defined reduction wave; one graphene oxide nanoplatelet of dimension 50 × 50 nm can provide a reduction signal by accepting ~22 000 electrons. We demonstrate that by using graphene oxide nanoplatelets as an inherently electroactive label, we can detect thrombin in the concentration range of 3 pM–0.3 μM, with good selectivity of the aptamer towards interferences by bovine serum albumin, immunoglobulin G and avidin. Therefore, the inherently electroactive graphene oxide nanoplatelets are a material which can serve as an electroactive label, in a manner similar to metallic nanoparticles. 2013-10-31T08:58:40Z 2019-12-06T20:03:57Z 2013-10-31T08:58:40Z 2019-12-06T20:03:57Z 2013 2013 Journal Article Loo, A. H., Bonanni, A., & Pumera, M. (2013). Thrombin aptasensing with inherently electroactive graphene oxide nanoplatelets as labels. Nanoscale, 5(11), 4758-4762. https://hdl.handle.net/10356/99155 http://hdl.handle.net/10220/17179 10.1039/c3nr00511a en Nanoscale
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Nanotechnology
spellingShingle DRNTU::Engineering::Nanotechnology
Loo, Adeline Huiling
Bonanni, Alessandra
Pumera, Martin
Thrombin aptasensing with inherently electroactive graphene oxide nanoplatelets as labels
description Graphene and its associated materials are commonly used as the transducing platform in biosensing. We propose a different approach for the application of graphene in biosensing. Here, we utilized graphene oxide nanoplatelets as the inherently electroactive labels for the aptasensing of thrombin. The basis of detection lies in the ability of graphene oxide to be electrochemically reduced, thereby providing a well-defined reduction wave; one graphene oxide nanoplatelet of dimension 50 × 50 nm can provide a reduction signal by accepting ~22 000 electrons. We demonstrate that by using graphene oxide nanoplatelets as an inherently electroactive label, we can detect thrombin in the concentration range of 3 pM–0.3 μM, with good selectivity of the aptamer towards interferences by bovine serum albumin, immunoglobulin G and avidin. Therefore, the inherently electroactive graphene oxide nanoplatelets are a material which can serve as an electroactive label, in a manner similar to metallic nanoparticles.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Loo, Adeline Huiling
Bonanni, Alessandra
Pumera, Martin
format Article
author Loo, Adeline Huiling
Bonanni, Alessandra
Pumera, Martin
author_sort Loo, Adeline Huiling
title Thrombin aptasensing with inherently electroactive graphene oxide nanoplatelets as labels
title_short Thrombin aptasensing with inherently electroactive graphene oxide nanoplatelets as labels
title_full Thrombin aptasensing with inherently electroactive graphene oxide nanoplatelets as labels
title_fullStr Thrombin aptasensing with inherently electroactive graphene oxide nanoplatelets as labels
title_full_unstemmed Thrombin aptasensing with inherently electroactive graphene oxide nanoplatelets as labels
title_sort thrombin aptasensing with inherently electroactive graphene oxide nanoplatelets as labels
publishDate 2013
url https://hdl.handle.net/10356/99155
http://hdl.handle.net/10220/17179
_version_ 1681037421523763200