Dynamic modelling of cell death during biofilm development

Biofilms are currently recognised as the predominant bacterial life-style and it has been suggested that biofilm development is influenced by a number of different processes such as adhesion, detachment, mass transport, quorum sensing, cell death and active dispersal. One of the least understood pro...

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Main Authors: Fagerlind, Magnus G., Webb, Jeremy S., Barraud, Nicolas, McDougald, Diane, Jansson, Andreas, Nilsson, Patric, Harlén, Mikael, Kjelleberg, Staffan, Rice, Scott Alan
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/99230
http://hdl.handle.net/10220/17166
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-992302020-03-07T12:47:10Z Dynamic modelling of cell death during biofilm development Fagerlind, Magnus G. Webb, Jeremy S. Barraud, Nicolas McDougald, Diane Jansson, Andreas Nilsson, Patric Harlén, Mikael Kjelleberg, Staffan Rice, Scott Alan DRNTU::Science::Biological sciences Biofilms are currently recognised as the predominant bacterial life-style and it has been suggested that biofilm development is influenced by a number of different processes such as adhesion, detachment, mass transport, quorum sensing, cell death and active dispersal. One of the least understood processes and its effects on biofilm development is cell death. However, experimental studies suggest that bacterial death is an important process during biofilm development and many studies show a relationship between cell death and dispersal in microbial biofilms. We present a model of the process of cell death during biofilm development, with a particular focus on the spatial localisation of cell death or cell damage. Three rules governing cell death or cell damage were evaluated which compared the effects of starvation, damage accumulation, and viability during biofilm development and were also used to design laboratory based experiments to test the model. Results from model simulations show that actively growing biofilms develop steep nutrient gradients within the interior of the biofilm that affect neighbouring microcolonies resulting in cell death and detachment. Two of the rules indicated that high substrate concentrations lead to accelerated cell death, in contrast to the third rule, based on the accumulation of damage, which predicted earlier cell death for biofilms grown with low substrate concentrations. Comparison of the modelling results with experimental results suggests that cell death is favoured under low nutrient conditions and that the accumulation of damage may be the main cause of cell death during biofilm development. 2013-10-31T08:16:43Z 2019-12-06T20:04:53Z 2013-10-31T08:16:43Z 2019-12-06T20:04:53Z 2011 2011 Journal Article Fagerlind, M. G., Webb, J. S., Barraud, N., McDougald, D., Jansson, A., Nilsson, P., et al. (2011). Dynamic modelling of cell death during biofilm development. Journal of theoretical biology, 295, 23-36. 0022-5193 https://hdl.handle.net/10356/99230 http://hdl.handle.net/10220/17166 10.1016/j.jtbi.2011.10.007 en Journal of theoretical biology
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Fagerlind, Magnus G.
Webb, Jeremy S.
Barraud, Nicolas
McDougald, Diane
Jansson, Andreas
Nilsson, Patric
Harlén, Mikael
Kjelleberg, Staffan
Rice, Scott Alan
Dynamic modelling of cell death during biofilm development
description Biofilms are currently recognised as the predominant bacterial life-style and it has been suggested that biofilm development is influenced by a number of different processes such as adhesion, detachment, mass transport, quorum sensing, cell death and active dispersal. One of the least understood processes and its effects on biofilm development is cell death. However, experimental studies suggest that bacterial death is an important process during biofilm development and many studies show a relationship between cell death and dispersal in microbial biofilms. We present a model of the process of cell death during biofilm development, with a particular focus on the spatial localisation of cell death or cell damage. Three rules governing cell death or cell damage were evaluated which compared the effects of starvation, damage accumulation, and viability during biofilm development and were also used to design laboratory based experiments to test the model. Results from model simulations show that actively growing biofilms develop steep nutrient gradients within the interior of the biofilm that affect neighbouring microcolonies resulting in cell death and detachment. Two of the rules indicated that high substrate concentrations lead to accelerated cell death, in contrast to the third rule, based on the accumulation of damage, which predicted earlier cell death for biofilms grown with low substrate concentrations. Comparison of the modelling results with experimental results suggests that cell death is favoured under low nutrient conditions and that the accumulation of damage may be the main cause of cell death during biofilm development.
format Article
author Fagerlind, Magnus G.
Webb, Jeremy S.
Barraud, Nicolas
McDougald, Diane
Jansson, Andreas
Nilsson, Patric
Harlén, Mikael
Kjelleberg, Staffan
Rice, Scott Alan
author_facet Fagerlind, Magnus G.
Webb, Jeremy S.
Barraud, Nicolas
McDougald, Diane
Jansson, Andreas
Nilsson, Patric
Harlén, Mikael
Kjelleberg, Staffan
Rice, Scott Alan
author_sort Fagerlind, Magnus G.
title Dynamic modelling of cell death during biofilm development
title_short Dynamic modelling of cell death during biofilm development
title_full Dynamic modelling of cell death during biofilm development
title_fullStr Dynamic modelling of cell death during biofilm development
title_full_unstemmed Dynamic modelling of cell death during biofilm development
title_sort dynamic modelling of cell death during biofilm development
publishDate 2013
url https://hdl.handle.net/10356/99230
http://hdl.handle.net/10220/17166
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