Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility

We have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjus...

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Main Authors: Qian, Chen, Wong, Chui Ching, Swarup, Sanjay, Chiam, Keng-Hwee
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/99308
http://hdl.handle.net/10220/17396
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-993082022-02-16T16:28:44Z Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility Qian, Chen Wong, Chui Ching Swarup, Sanjay Chiam, Keng-Hwee DRNTU::Science::Biological sciences::Microbiology We have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of the cell's rotational trajectories. The second applies piecewise linear approximation to the accumulated rotation curve to reduce noise and separate the motion of bacteria into phases. Thus, it can separate counterclockwise (CCW) and clockwise (CW) rotations distinctly and measure rotational speed accurately. Using this program, we analyzed the properties of tethered Pseudomonas aeruginosa and Pseudomonas putida cells for the first time. We found that the Pseudomonas flagellar motor spends equal time in both CCW and CW phases and that it rotates with the same speed in both phases. In addition, we discovered that the cell body can remain stationary for short periods of time, leading to the existence of a third phase of the flagellar motor which we call “pause.” In addition, P. aeruginosa cells adopt longer run lengths, fewer pause frequencies, and shorter pause durations as part of their chemotactic response. We propose that one purpose of the pause phase is to allow the cells to turn at a large angle, where we show that pause durations in free-swimming cells positively correlate with turn angle sizes. Taken together, our results suggest a new “run-reverse-turn” paradigm for polar-flagellated Pseudomonas motility that is different from the “run-and-tumble” paradigm established for peritrichous Escherichia coli. 2013-11-07T07:28:43Z 2019-12-06T20:05:37Z 2013-11-07T07:28:43Z 2019-12-06T20:05:37Z 2013 2013 Journal Article Qian, C., Wong, C. C., Swarup, S., & Chiam, K. H. (2013). Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility. Applied and environmental microbiology, 79(15), 4734-4743. 0099-2240 https://hdl.handle.net/10356/99308 http://hdl.handle.net/10220/17396 10.1128/AEM.01027-13 23728820 en Applied and environmental microbiology
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology
spellingShingle DRNTU::Science::Biological sciences::Microbiology
Qian, Chen
Wong, Chui Ching
Swarup, Sanjay
Chiam, Keng-Hwee
Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility
description We have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of the cell's rotational trajectories. The second applies piecewise linear approximation to the accumulated rotation curve to reduce noise and separate the motion of bacteria into phases. Thus, it can separate counterclockwise (CCW) and clockwise (CW) rotations distinctly and measure rotational speed accurately. Using this program, we analyzed the properties of tethered Pseudomonas aeruginosa and Pseudomonas putida cells for the first time. We found that the Pseudomonas flagellar motor spends equal time in both CCW and CW phases and that it rotates with the same speed in both phases. In addition, we discovered that the cell body can remain stationary for short periods of time, leading to the existence of a third phase of the flagellar motor which we call “pause.” In addition, P. aeruginosa cells adopt longer run lengths, fewer pause frequencies, and shorter pause durations as part of their chemotactic response. We propose that one purpose of the pause phase is to allow the cells to turn at a large angle, where we show that pause durations in free-swimming cells positively correlate with turn angle sizes. Taken together, our results suggest a new “run-reverse-turn” paradigm for polar-flagellated Pseudomonas motility that is different from the “run-and-tumble” paradigm established for peritrichous Escherichia coli.
format Article
author Qian, Chen
Wong, Chui Ching
Swarup, Sanjay
Chiam, Keng-Hwee
author_facet Qian, Chen
Wong, Chui Ching
Swarup, Sanjay
Chiam, Keng-Hwee
author_sort Qian, Chen
title Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility
title_short Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility
title_full Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility
title_fullStr Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility
title_full_unstemmed Bacterial tethering analysis reveals a "run-reverse-turn" mechanism for Pseudomonas species motility
title_sort bacterial tethering analysis reveals a "run-reverse-turn" mechanism for pseudomonas species motility
publishDate 2013
url https://hdl.handle.net/10356/99308
http://hdl.handle.net/10220/17396
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