The DNA uptake ATPase PilF of Thermus thermophilus : a reexamination of the zinc content

• Main Authors: Nies, Dietrich H., Salzer, Ralf, Herzberg, Martin, Biuković, Goran, Grüber, Gerhard, Müller, Volker, Averhoff, Beate
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences::Microbiology
• Online Access: https://hdl.handle.net/10356/99636; http://hdl.handle.net/10220/17455
• Institution: Nanyang Technological University

The DNA-translocator ATPase PilF of Thermus thermophilus HB27 is a hexamer built by six identical subunits. Despite the presence of a conserved zinc-binding site in every subunit, only one zinc atom per hexamer was found. Re-examination of the zinc content of PilF purified from cells grown in comple...