Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa

While antibiotic resistance in bacteria is rapidly increasing, the development of new antibiotics has decreased in recent years. Antivirulence drugs disarming rather than killing pathogens have been proposed to alleviate the problem of resistance inherent to existing biocidal antibiotics. Here, we r...

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Main Authors: Mohanty, Anee, Kathawala, Mustafa Hussain, Zhang, Jianhua, Yang, Liang, Kjelleberg, Staffan, Cao, Bin, Chen, William Wei Ning, Loo, Joachim Say Chye
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/99766
http://hdl.handle.net/10220/17820
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-997662020-09-21T11:33:32Z Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa Mohanty, Anee Kathawala, Mustafa Hussain Zhang, Jianhua Yang, Liang Kjelleberg, Staffan Cao, Bin Chen, William Wei Ning Loo, Joachim Say Chye School of Civil and Environmental Engineering Singapore Centre for Environmental Life Sciences Engineering DRNTU::Engineering::Chemical engineering::Biotechnology While antibiotic resistance in bacteria is rapidly increasing, the development of new antibiotics has decreased in recent years. Antivirulence drugs disarming rather than killing pathogens have been proposed to alleviate the problem of resistance inherent to existing biocidal antibiotics. Here, we report a nontoxic biogenic nanomaterial as a novel antivirulence agent to combat bacterial infections caused by Pseudomonas aeruginosa. We synthesized, in an environmentally benign fashion, tellurium nanorods (TeNRs) using the metal-reducing bacterium Shewanella oneidensis, and found that the biogenic TeNRs could effectively inhibit the production of pyoverdine, one of the most important virulence factors in P. aeruginosa. Our results suggest that amyloids and extracellular polysaccharides Pel and Psl are not involved in the interactions between P. aeruginosa and the biogenic TeNRs, while flagellar movement plays an important role in the cell-TeNRs interaction. We further showed that the TeNRs (up to 100 µg/ml) did not exhibit cytotoxicity to human bronchial epithelial cells and murine macrophages. Thus, biogenic TeNRs hold promise as a novel antivirulence agent against P. aeruginosa. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) Accepted version 2013-11-25T02:56:17Z 2019-12-06T20:11:11Z 2013-11-25T02:56:17Z 2019-12-06T20:11:11Z 2013 2013 Journal Article Mohanty, A., Kathawala, M. H., Zhang, J., Chen, W. N. W., Loo, S. C. J., Kjelleberg, S., et al. (2013). Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa . Biotechnology and Bioengineering, 111(5), 858–865. 0006-3592 https://hdl.handle.net/10356/99766 http://hdl.handle.net/10220/17820 10.1002/bit.25147 en Biotechnology and bioengineering © 2013 Wiley Periodicals, Inc. This is the author created version of a work that has been peer reviewed and accepted for publication by Biotechnology and bioengineering, Wiley Periodicals, Inc. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/bit.25147]. 23 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Chemical engineering::Biotechnology
spellingShingle DRNTU::Engineering::Chemical engineering::Biotechnology
Mohanty, Anee
Kathawala, Mustafa Hussain
Zhang, Jianhua
Yang, Liang
Kjelleberg, Staffan
Cao, Bin
Chen, William Wei Ning
Loo, Joachim Say Chye
Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa
description While antibiotic resistance in bacteria is rapidly increasing, the development of new antibiotics has decreased in recent years. Antivirulence drugs disarming rather than killing pathogens have been proposed to alleviate the problem of resistance inherent to existing biocidal antibiotics. Here, we report a nontoxic biogenic nanomaterial as a novel antivirulence agent to combat bacterial infections caused by Pseudomonas aeruginosa. We synthesized, in an environmentally benign fashion, tellurium nanorods (TeNRs) using the metal-reducing bacterium Shewanella oneidensis, and found that the biogenic TeNRs could effectively inhibit the production of pyoverdine, one of the most important virulence factors in P. aeruginosa. Our results suggest that amyloids and extracellular polysaccharides Pel and Psl are not involved in the interactions between P. aeruginosa and the biogenic TeNRs, while flagellar movement plays an important role in the cell-TeNRs interaction. We further showed that the TeNRs (up to 100 µg/ml) did not exhibit cytotoxicity to human bronchial epithelial cells and murine macrophages. Thus, biogenic TeNRs hold promise as a novel antivirulence agent against P. aeruginosa.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Mohanty, Anee
Kathawala, Mustafa Hussain
Zhang, Jianhua
Yang, Liang
Kjelleberg, Staffan
Cao, Bin
Chen, William Wei Ning
Loo, Joachim Say Chye
format Article
author Mohanty, Anee
Kathawala, Mustafa Hussain
Zhang, Jianhua
Yang, Liang
Kjelleberg, Staffan
Cao, Bin
Chen, William Wei Ning
Loo, Joachim Say Chye
author_sort Mohanty, Anee
title Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa
title_short Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa
title_full Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa
title_fullStr Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa
title_full_unstemmed Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa
title_sort biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in pseudomonas aeruginosa
publishDate 2013
url https://hdl.handle.net/10356/99766
http://hdl.handle.net/10220/17820
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