Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa
While antibiotic resistance in bacteria is rapidly increasing, the development of new antibiotics has decreased in recent years. Antivirulence drugs disarming rather than killing pathogens have been proposed to alleviate the problem of resistance inherent to existing biocidal antibiotics. Here, we r...
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sg-ntu-dr.10356-997662020-09-21T11:33:32Z Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa Mohanty, Anee Kathawala, Mustafa Hussain Zhang, Jianhua Yang, Liang Kjelleberg, Staffan Cao, Bin Chen, William Wei Ning Loo, Joachim Say Chye School of Civil and Environmental Engineering Singapore Centre for Environmental Life Sciences Engineering DRNTU::Engineering::Chemical engineering::Biotechnology While antibiotic resistance in bacteria is rapidly increasing, the development of new antibiotics has decreased in recent years. Antivirulence drugs disarming rather than killing pathogens have been proposed to alleviate the problem of resistance inherent to existing biocidal antibiotics. Here, we report a nontoxic biogenic nanomaterial as a novel antivirulence agent to combat bacterial infections caused by Pseudomonas aeruginosa. We synthesized, in an environmentally benign fashion, tellurium nanorods (TeNRs) using the metal-reducing bacterium Shewanella oneidensis, and found that the biogenic TeNRs could effectively inhibit the production of pyoverdine, one of the most important virulence factors in P. aeruginosa. Our results suggest that amyloids and extracellular polysaccharides Pel and Psl are not involved in the interactions between P. aeruginosa and the biogenic TeNRs, while flagellar movement plays an important role in the cell-TeNRs interaction. We further showed that the TeNRs (up to 100 µg/ml) did not exhibit cytotoxicity to human bronchial epithelial cells and murine macrophages. Thus, biogenic TeNRs hold promise as a novel antivirulence agent against P. aeruginosa. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) Accepted version 2013-11-25T02:56:17Z 2019-12-06T20:11:11Z 2013-11-25T02:56:17Z 2019-12-06T20:11:11Z 2013 2013 Journal Article Mohanty, A., Kathawala, M. H., Zhang, J., Chen, W. N. W., Loo, S. C. J., Kjelleberg, S., et al. (2013). Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa . Biotechnology and Bioengineering, 111(5), 858–865. 0006-3592 https://hdl.handle.net/10356/99766 http://hdl.handle.net/10220/17820 10.1002/bit.25147 en Biotechnology and bioengineering © 2013 Wiley Periodicals, Inc. This is the author created version of a work that has been peer reviewed and accepted for publication by Biotechnology and bioengineering, Wiley Periodicals, Inc. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/bit.25147]. 23 p. application/pdf |
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DRNTU::Engineering::Chemical engineering::Biotechnology Mohanty, Anee Kathawala, Mustafa Hussain Zhang, Jianhua Yang, Liang Kjelleberg, Staffan Cao, Bin Chen, William Wei Ning Loo, Joachim Say Chye Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa |
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While antibiotic resistance in bacteria is rapidly increasing, the development of new antibiotics has decreased in recent years. Antivirulence drugs disarming rather than killing pathogens have been proposed to alleviate the problem of resistance inherent to existing biocidal antibiotics. Here, we report a nontoxic biogenic nanomaterial as a novel antivirulence agent to combat bacterial infections caused by Pseudomonas aeruginosa. We synthesized, in an environmentally benign fashion, tellurium nanorods (TeNRs) using the metal-reducing bacterium Shewanella oneidensis, and found that the biogenic TeNRs could effectively inhibit the production of pyoverdine, one of the most important virulence factors in P. aeruginosa. Our results suggest that amyloids and extracellular polysaccharides Pel and Psl are not involved in the interactions between P. aeruginosa and the biogenic TeNRs, while flagellar movement plays an important role in the cell-TeNRs interaction. We further showed that the TeNRs (up to 100 µg/ml) did not exhibit cytotoxicity to human bronchial epithelial cells and murine macrophages. Thus, biogenic TeNRs hold promise as a novel antivirulence agent against P. aeruginosa. |
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School of Civil and Environmental Engineering |
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School of Civil and Environmental Engineering Mohanty, Anee Kathawala, Mustafa Hussain Zhang, Jianhua Yang, Liang Kjelleberg, Staffan Cao, Bin Chen, William Wei Ning Loo, Joachim Say Chye |
format |
Article |
author |
Mohanty, Anee Kathawala, Mustafa Hussain Zhang, Jianhua Yang, Liang Kjelleberg, Staffan Cao, Bin Chen, William Wei Ning Loo, Joachim Say Chye |
author_sort |
Mohanty, Anee |
title |
Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa |
title_short |
Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa |
title_full |
Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa |
title_fullStr |
Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa |
title_full_unstemmed |
Biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in Pseudomonas aeruginosa |
title_sort |
biogenic tellurium nanorods as a novel antivirulence agent inhibiting pyoverdine production in pseudomonas aeruginosa |
publishDate |
2013 |
url |
https://hdl.handle.net/10356/99766 http://hdl.handle.net/10220/17820 |
_version_ |
1681057532551888896 |