Myo1e impairment results in actin reorganization, podocyte dysfunction, and proteinuria in zebrafish and cultured podocytes

• Main Authors: Mao J., Wang D., Mataleena P., He B., Niu D., Katayama K., Xu X., Ojala J.R.M., Wang W., Shu Q., Du L., Liu A., Pikkarainen T., Patrakka J., Tryggvason K.
• Other Authors: DUKE-NUS MEDICAL SCHOOL
• Format: Article
• Published: 2019
• Subjects: actin; antisense oligonucleotide; complementary DNA; messenger RNA; myosin; myosin1e; nephrin; podocin; short hairpin RNA; synaptopodin; unclassified drug; vitellogenin; fluorescein isothiocyanate; MYO1E protein, human; Myo1e protein, mouse; Myo1e protein, zebrafish; myosin I; transferrin; zebrafish protein; actin filament; animal cell; animal tissue; Article; cardiovascular disease; cell adhesion; cell culture; cell migration; cell motility; cell proliferation; cell shape; cell structure; congenital nephrotic syndrome; controlled study; cytoplasm; cytoskeleton; down regulation; embryo; endocytosis; enzyme linked immunosorbent assay; exon; fluorescence microscopy; gene sequence; glomerular filtration barrier; glomerulus basement membrane; histopathology; human; human cell; human tissue; immortalized cell line; immunofluorescence; incubation time; kidney cyst; kidney disease; kidney failure; larva; mass spectrometry; mouse; nonhuman; Northern blotting; pericarditis; phenotype; podocyte; protein analysis; protein expression; protein localization; proteinuria; reverse transcription polymerase chain reaction; risk factor; RNA splicing; supernatant; upregulation; zebra fish; animal; cell count; cell migration assay; fertilization; gene silencing; glomerulus; metabolism; pathology; Danio rerio; Actins; Animals; Cell Count; Cell Migration Assays; Cell Proliferation; Cell Shape; Cells, Cultured; Cytoskeleton; Down-Regulation; Endocytosis; Fertilization; Fluorescein-5-isothiocyanate; Gene Knockdown Techniques; Humans; Kidney Diseases; Kidney Glomerulus; Mice; Myosin Type I; Myosins; Podocytes; Proteinuria; Transferrin; Zebrafish; Zebrafish Proteins
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161275
• Institution: National University of Singapore

10.1371/journal.pone.0072750