Serious overestimation in quantitative pcr by circular (supercoiled) plasmid standard: Microalgal pcnaas the model gene

• Main Authors: Hou Y., Zhang H., Miranda L., Lin S.
• Other Authors: TROPICAL MARINE SCIENCE INSTITUTE
• Published: 2019
• Subjects: circular DNA; complementary DNA; cycline; amplicon; article; cell proliferation; controlled study; diatom; DNA supercoiling; DNA template; microalga; nonhuman; nucleotide sequence; plasmid; quantitative analysis; Thalassiosira pseudonana; gene library; genetic procedures; genetics; genome; metabolism; methodology; molecular cloning; polymerase chain reaction; standard; Bacillariophyta; Eukaryota; Cloning, Molecular; DNA, Complementary; Gene Library; Genetic Techniques; Genome; Microalgae; Plasmids; Polymerase Chain Reaction; Proliferating Cell Nuclear Antigen
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161815
• Institution: National University of Singapore

10.1371/journal.pone.0009545