Four human Plasmodium species quantification using droplet digital PCR

• Main Authors: Srisutham S., Saralamba N., Malleret B., Rénia L., Dondorp A.M., Imwong M.
• Other Authors: MICROBIOLOGY AND IMMUNOLOGY
• Format: Article
• Published: Public Library of Science 2020
• Subjects: genomic DNA; RNA 18S; emulsion; protozoal DNA; adult; Article; blood sampling; blood volume; Cambodia; concentration (parameters); controlled study; cost benefit analysis; diagnostic accuracy; diagnostic equipment; diagnostic test accuracy study; differential diagnosis; droplet digital polymerase chain reaction; duplex droplet digital polymerase chain reaction; gene amplification; human; intermethod comparison; limit of detection; major clinical study; malaria; malaria falciparum; mixed infection; nonhuman; parasite identification; parasite load; Plasmodium falciparum; Plasmodium malariae; Plasmodium malariae infection; Plasmodium ovale; Plasmodium ovale malaria; Plasmodium vivax; Plasmodium vivax malaria; polymerase chain reaction; quantitative analysis; reverse transcription polymerase chain reaction; sensitivity and specificity; species identification; adolescent; female; genetics; isolation and purification; Malaria, Falciparum; Malaria, Vivax; male; middle aged; multiplex polymerase chain reaction; parasitemia; parasitology; procedures; real time polymerase chain reaction; Adolescent; Adult; Diagnosis, Differential; DNA, Protozoan; Emulsions; Female; Humans; Malaria; Male; Middle Aged; Multiplex Polymerase Chain Reaction; Parasitemia; Real-Time Polymerase Chain Reaction; RNA, Ribosomal, 18S; Sensitivity and Specificity
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/166012
• Institution: National University of Singapore

10.1371/journal.pone.0175771