Generation and characterization of a novel recombinant antibody against 15-ketocholestane isolated by phage-display

• Main Authors: Omedul Islam M., Lim Y.T., Chan C.E.Z., Cazenave-Gassiot A., Ludovic Croxford J., Wenk M.R., Macary P.A., Hanson B.J.
• Other Authors: BIOCHEMISTRY
• Format: Article
• Published: 2020
• Subjects: antigen; biological marker; immunoglobulin G antibody; monoclonal antibody; monoclonal antibody 2E9; oxysterol; oxysterol 15 ketocholestane; recombinant oxysterol 15 ketocholestane monoclonal antibody; unclassified drug; cholest 4 en 3 one; cholest-8(14)-en-3-ol-15-one; cholesterol; peptide library; allergic encephalomyelitis; animal model; antibody detection; antibody isolation; antibody production; antibody specificity; antigen binding; article; binding affinity; controlled study; disease marker; feasibility study; human; immunogenicity; mass spectrometry; mouse; nonhuman; phage display; analogs and derivatives; animal; blood; cell surface display; diagnostic use; Encephalomyelitis, Autoimmune, Experimental; immunology; multiple sclerosis; Animals; Antibodies, Monoclonal; Biological Markers; Cell Surface Display Techniques; Cholestenones; Cholesterol; Humans; Mice; Multiple Sclerosis; Peptide Library
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/174658
• Institution: National University of Singapore

10.3390/ijms13044937